Results 11 to 20 of about 505,963 (341)

The coherent dynamics of photoexcited green fluorescent proteins [PDF]

Physical Review Letters, 2001
The coherent dynamics of vibronic wave packets in the green fluorescent protein is reported. At room temperature the non-stationary dynamics following impulsive photoexcitation displays an oscillating optical transmissivity pattern with components at 67 fs (497 cm-1) and 59 fs (593 cm-1).
R. Cinelli, V. Tozzini, V. Pellegrini, F. Beltram, CERULLO, GIULIO NICOLA, ZAVELANI ROSSI, MARGHERITA, DE SILVESTRI, SANDRO, M. Tyagi, M. Giacca   +8 more
arxiv   +7 more sources

Red fluorescent proteins engineered from green fluorescent proteins. [PDF]

Proc Natl Acad Sci U S A, 2023
Fluorescent proteins (FPs) form a fluorophore through autocatalysis from three consecutive amino acid residues within a polypeptide chain. The two major groups, green FPs (GFPs) and red FPs (RFPs), have distinct fluorophore structures; RFPs have an extended π-conjugation system with an additional double bond.
Imamura H, Otsubo S, Nishida M, Takekawa N, Imada K.   +4 more
europepmc   +3 more sources

Fluorescence polarization of green fluorescence protein [PDF]

Proceedings of the National Academy of Sciences, 2002
We report here the striking anisotropy of fluorescence exhibited by crystals of native green fluorescence protein (GFP). The crystals were generated by water dialysis of highly purified GFP obtained from the jellyfish Aequorea .
Shinya Inoué, Mykhailo Shribak, Phong T. Tran, Osamu Shimomura, Makoto Goda   +4 more
openaire   +4 more sources

Photoactivation of green fluorescent protein [PDF]

Current Biology, 1997
Considerable insight into many cell biological processes can be obtained by following the turnover of individual protein species in time and space. In living cells, this has been achieved by following fluorescence recovery after local microbeam photobleaching of microinjected fluorescently labelled protein (see, for example, [1xSpindle microtubule ...
Paul Nurse, Kenneth E. Sawin
openaire   +3 more sources

Origin of the Intrinsic Fluorescence of the Green Fluorescent Protein [PDF]

Journal of the American Chemical Society, 2017
Green fluorescent protein, GFP, has revolutionized biology, due to its use in bioimaging. It is widely accepted that the protein environment makes its chromophore fluoresce, whereas the fluorescence is completely lost when the native chromophore is taken out of GFP.
Annette Svendsen, Hjalte V. Kiefer, Henrik B. Pedersen, Anastasia V. Bochenkova, Lars H. Andersen   +4 more
openaire   +4 more sources

Imaging the Environment of Green Fluorescent Protein [PDF]

Biophysical Journal, 2002
An emerging theme in cell biology is that cell surface receptors need to be considered as part of supramolecular complexes of proteins and lipids facilitating specific receptor conformations and distinct distributions, e.g., at the immunological synapse. Thus, a new goal is to develop bioimaging that not only locates proteins in live cells but can also
Suhling, K, Siegel, J, Phillips, D, Davis, D M, LevequeFort, S, Webb, S E D, French, P M W   +6 more
openaire   +5 more sources

Photochromicity and Fluorescence Lifetimes of Green Fluorescent Protein [PDF]

The Journal of Physical Chemistry B, 1999
The green fluorescent protein (GFP) of the bioluminescent jellyfish Aequorea and its mutants have gained widespread usage as an indicator of structure and function within cells. Proton transfer has been implicated in the complex photophysics of the wild-type molecule, exhibiting a protonated A species excited at 400 nm, and two deprotonated excited ...
Striker, G., Subramaniam, V., Seidel, C., Volkmer, A.   +3 more
openaire   +3 more sources

Variants of Green Fluorescent Protein GFPxm [PDF]

Marine Biotechnology, 2006
As research progresses, fluorescent proteins useful for optical marking will evolve toward brighter, monomeric forms that are more diverse in color. We previously reported a new fluorescent protein from Aequorea macrodactyla, GFPxm, that exhibited many characteristics similar to wild-type green fluorescent protein (GFP).
Luo, W. X., Cheng, T., Guan, B. Q., Li, S. W., Miao, J., Zhang, J., Xia, N. S.   +6 more
openaire   +3 more sources

Using photoactivatable fluorescent protein Dendra2 to track protein movement

BioTechniques, 2007
Photoactivatable fluorescent proteins are capable of dramatic changes in fluorescent properties in response to specific light irradiation. For example, they can be converted from cyan to green, or from green to red, or from nonfluorescent to a brightly ...
Dmitriy M. Chudakov, Sergey Lukyanov, Konstantin A. Lukyanov   +2 more
doaj   +1 more source

Evolution of a fluorinated green fluorescent protein [PDF]

Proceedings of the National Academy of Sciences, 2007
The fluorescence of bacterial cells expressing a variant (GFPm) of the green fluorescent protein (GFP) was reduced to background levels by global replacement of the leucine residues of GFPm by 5,5,5-trifluoroleucine. Eleven rounds of random mutagenesis and screening via fluorescence-activated cell sorting yielded a GFP mutant containing 20
Yoo, Tae-Hyeon, Link, A. James, Tirrell, David A.   +2 more
openaire   +5 more sources