Results 11 to 20 of about 491,462 (265)
Red fluorescent proteins engineered from green fluorescent proteins. [PDF]
Proc Natl Acad Sci U S A, 2023 Fluorescent proteins (FPs) form a fluorophore through autocatalysis from three consecutive amino acid residues within a polypeptide chain. The two major groups, green FPs (GFPs) and red FPs (RFPs), have distinct fluorophore structures; RFPs have an extended π-conjugation system with an additional double bond.
Imamura H +4 more
europepmc +3 more sources
Generation of photonic entanglement in green fluorescent proteins [PDF]
Nature Communications, 2017 Quantum-enhanced applications such as quantum spectroscopy of biological samples could take advantage from in situ generation of quantum states of light.
Siyuan Shi, Prem Kumar, Kim Fook Lee
doaj +2 more sources
Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. [PDF]
PLoS ONE, 2013 Fluorescent protein fusions are a powerful tool to monitor the localization and trafficking of proteins. Such studies are particularly easy to carry out in the budding yeast Saccharomyces cerevisiae due to the ease with which tags can be introduced into ...
Sidae Lee, Wendell A Lim, Kurt S Thorn
doaj +1 more source
Engineering an efficient and bright split Corynactis californica green fluorescent protein
Scientific Reports, 2021 Split green fluorescent protein (GFP) has been used in a panoply of cellular biology applications to study protein translocation, monitor protein solubility and aggregation, detect protein–protein interactions, enhance protein crystallization, and even ...
Hau B. Nguyen +2 more
doaj +1 more source
Fluorescence polarization of green fluorescence protein [PDF]
Proceedings of the National Academy of Sciences, 2002 We report here the striking anisotropy of fluorescence exhibited by crystals of native green fluorescence protein (GFP). The crystals were generated by water dialysis of highly purified GFP obtained from the jellyfish Aequorea .
Shinya, Inoué +4 more
openaire +3 more sources
Very bright green fluorescent proteins from the Pontellid copepod Pontella mimocerami. [PDF]
PLoS ONE, 2010 Fluorescent proteins (FP) homologous to the green fluorescent protein (GFP) from the jellyfish Aequorea victoria have revolutionized biomedical research due to their usefulness as genetically encoded fluorescent labels. Fluorescent proteins from copepods
Marguerite E Hunt +3 more
doaj +1 more source
BioTechniques, 2023 Fluorescent proteins, such as green fluorescent proteins, are invaluable tools for detecting and quantifying gene expression in high-throughput reporter gene assays. However, they introduce significant inaccuracies in studies involving microaerobiosis or
Lucas Pedraz, Eduard Torrents
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eLife, 2022 Using a neural network to predict how green fluorescent proteins respond to genetic mutations illuminates properties that could help design new proteins.
Grzegorz Kudla, Marcin Plech
doaj +1 more source
Rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins
Communications Biology, 2021 Zhang, Redington & Gong develop FRET-based ratiometric calcium sensors with pairs of green and red fluorescent proteins. Their best performers, Twitch-GR and Twitch-NR inherit the superior photophysical properties of their constituent fluorescent ...
Diming Zhang +2 more
doaj +1 more source
Frontiers in Marine Science, 2021 Since the initial discovery of Aqueoria victoria’s green fluorescence off the coast of Washington’s Puget Sound, biofluorescent marine organisms have been found across the globe.
Andrew M. Guarnaccia +10 more
doaj +1 more source