Results 21 to 30 of about 505,963 (341)

A method for fixing and paraffin embedding tissue to retain the natural fluorescence of reporter proteins

open access: yesBioTechniques, 2015
Green fluorescent protein (GFP) and its derivatives are routinely employed as surrogate markers for gene expression and lineage tracing in genetically engineered mice.
Akifumi Nakagawa   +5 more
doaj   +1 more source

Two-color in-resin CLEM of Epon-embedded cells using osmium resistant green and red fluorescent proteins

open access: yesScientific Reports, 2020
In-resin CLEM of Epon embedded samples can greatly simplify the correlation of fluorescent images with electron micrographs. The usefulness of this technique is limited at present by the low number of fluorescent proteins that resist CLEM processing ...
Isei Tanida   +5 more
doaj   +1 more source

Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum

open access: yesBioTechniques, 2006
O6-alkylguanine-DNA alkyltransferase (AGT) fusion proteins can be specifically and covalently labeled with fluorescent O6-benzylguanine (O6-BG) derivatives for multicolor live cell imaging approaches.
Antje Keppler   +3 more
doaj   +1 more source

Fluorescent proteins as tools to aid protein production

open access: yesMicrobial Cell Factories, 2005
Fluorescent proteins are genetically encoded, highly versatile reporters useful for monitoring various aspects of recombinant protein production. In addition to the widely popular green fluorescent protein (GFP) from Aequorea victoria, a variety of other
Su Wei
doaj   +1 more source

R26R-GR: a Cre-activable dual fluorescent protein reporter mouse. [PDF]

open access: yesPLoS ONE, 2012
Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level.
You-Tzung Chen   +21 more
doaj   +1 more source

Direction dependent mechanical unfolding and Green Fluorescent Protein as a force sensor [PDF]

open access: yesPhysical Review E 84, 021918 (2011), 2011
An Ising--like model of proteins is used to investigate the mechanical unfolding of the Green Fluorescent Protein along different directions. When the protein is pulled from its ends, we recover the major and minor unfolding pathways observed in experiments.
arxiv   +1 more source

Ultralow-threshold green fluorescent protein laser based on high Q microbubble resonators [PDF]

open access: yes, 2022
Biological lasers have attracted vast attention because of their potential medical application prospects, especially the low threshold biological laser, which can be used for ultrasensitive biological detection while ensuring that its luminous gain medium is not damaged by the high-energy pump light.
arxiv   +1 more source

Green fluorescent protein: A perspective [PDF]

open access: yesProtein Science, 2011
AbstractA brief personal perspective is provided for green fluorescent protein (GFP), covering the period 1994–2011. The topics discussed are primarily those in which my research group has made a contribution and include structure and function of the GFP polypeptide, the mechanism of fluorescence emission, excited state protein transfer, the design of ...
openaire   +3 more sources

Getting to grips with ammonium

open access: yeseLife, 2013
A fluorescent sensor that can monitor levels of extracellular ammonium has been made by using a fused green fluorescent protein to detect conformational changes in ammonium transport proteins.
Yi Wang, Jonathan A Javitch
doaj   +1 more source

Green Fluorescent Protein: discover, structure, purification, applications, and future prospects

open access: yesJournal of Scientific Research in Science, 2023
Green fluorescent protein (GFP) Firstly discovered from the jellyfish Aequorea victoriaand it was widely used in biological science as a universal fluorescent tag.
Hend A. Anter   +2 more
doaj   +1 more source

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