Fluorescence color diversity of great barrier reef corals [PDF]
Journal of Innovative Optical Health Sciences, 2015 A group of variously colored proteins belonging to the green fluorescent protein (GFP) family are responsible for coloring coral tissues. Corals of the Great Barrier Reef were studied with the custom-built fiber laser fluorescence spectrometers. Spectral
Grigory Lapshin +8 more
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Ultraviolet optical horn antennas for label-free detection of single proteins [PDF]
Nature Communications, Nature Publishing Group, 2022, 13, pp.1842, 2022 Single-molecule fluorescence techniques have revolutionized our ability to study proteins. However, the presence of a fluorescent label can alter the protein structure and/or modify its reaction with other species. To avoid the need for a fluorescent label, the intrinsic autofluorescence of proteins in the ultraviolet offers the benefits of ...
arxiv +1 more source
Combining single-molecule super-resolved localization microscopy with fluorescence polarization imaging to study cellular processes [PDF]
2021 J. Phys. Photonics 3 034010, 2020 Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled important insights into cellular processes through identifying orientational changes of biological molecules ...
arxiv +1 more source
Fluorescent proteins reveal what trypanosomes get up to inside the tsetse fly
Parasites & Vectors, 2019 The discovery and development of fluorescent proteins for the investigation of living cells and whole organisms has been a major advance in biomedical research.
Wendy Gibson, Lori Peacock
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Dynamical Decoherence and Memory Effects in Green Fluorescent Proteins by Dielectric Relaxation [PDF]
arXiv, 2022 In this article, we explore the dynamical decoherence of the chromophores within a green fluorescent protein when coupled to a finite-temperature dielectric environment. Such systems are of significant interest due to their anomalously long coherence lifetimes compared to other biomolecules.
arxiv
Sensitive detection of p65 homodimers using red-shifted and fluorescent protein-based FRET couples. [PDF]
PLoS ONE, 2007 BACKGROUND: Fluorescence Resonance Energy Transfer (FRET) between the green fluorescent protein (GFP) variants CFP and YFP is widely used for the detection of protein-protein interactions.
Joachim Goedhart +4 more
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Proton Pathways in Green Fluorescence Protein [PDF]
Biophysical Journal, 2005 Proton pathways in green fluorescent protein (GFP) are more extended than previously reported. In the x-ray data of wild-type GFP, a two-step exit pathway exists from the active site to the protein surface, controlled by a threonine switch. A proton entry pathway begins at a glutamate-lysine cluster around Glu-5, and extends all the way to the buried ...
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Pressure and temperature dependence of fluorescence anisotropy of Green Fluorescent Protein [PDF]
arXiv, 2022 We have studied the effect of high hydrostatic pressure and temperature on the steady state fluorescence anisotropy of Green Fluorescent Protein (GFP). We find that the fluorescence anisotropy of GFP at a constant temperature decreases with increasing pressure.
arxiv
Nonequilibrium dynamics of an exactly solvable Ising-like model and protein translocation [PDF]
Europhysics Letters 102, 10001 (2013), 2013 Using an Ising-like model of protein mechanical unfolding, we introduce a diffusive dynamics on its exactly known free energy profile, reducing the nonequilibrium dynamics of the model to a biased random walk. As an illustration, the model is then applied to the protein translocation phenomenon, taking inspiration from a recent experiment on the green ...
arxiv +1 more source
Green Fluorescent Protein Glows Gold [PDF]
Cell, 2008 The awarding of this year's Nobel Prize in Chemistry to Osamu Shimomura, Martin Chalfie, and Roger Tsien for their discovery and development of green fluorescent protein earns this humble jellyfish protein a place of honor in the biology research hall of fame.
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