Results 41 to 50 of about 505,963 (341)
Research in Pharmaceutical Sciences, 2019 Green fluorescent protein (GFP) has played an important role in biochemistry and cell biology as a reporter gene. It has been used to assess the potency of promoters for recombinant protein production. This investigation reveals evidences suggesting that
Zahra Mohammadi +4 more
doaj +1 more source
The molecular structure of green fluorescent protein [PDF]
Nature Biotechnology, 1996 The crystal structure of recombinant wild-type green fluorescent protein (GFP) has been solved to a resolution of 1.9 A by multiwavelength anomalous dispersion phasing methods. The protein is in the shape of a cylinder, comprising 11 strands of beta-sheet with an alpha-helix inside and short helical segments on the ends of the cylinder.
Larry G. Moss +2 more
openaire +2 more sources
BioTechniques, 2017 Protocol Summary Here we present an ethanol fixation protocol that allows for the direct visualization of the natural fluorescence of reporter proteins while maintaining excellent tissue histology.
Akifumi Nakagawa +5 more
doaj +1 more source
Photooxidation-guided ultrastructural identification and analysis of cells in neuronal tissue labeled with green fluorescent protein. [PDF]
PLoS ONE, 2013 The ultrastructural characterization of neuronal compartments in intact tissue labeled with green fluorescent protein (GFP) remains a frequently encountered challenge, despite work establishing photooxidation of GFP in cultured cells.
Heinz Horstmann +2 more
doaj +1 more source
Applications of Green Fluorescent Protein in Plants
BioTechniques, 1997 Green fluorescent protein (GFP) is increasingly being used in plant biology from the cellular level to whole plant level. At the cellular level, GFP is being used as an in vivo reporter to assess frequency of transient and stable transformation. GFP has also proven to be an invaluable tool in monitoring trafficking and subcellular localization of ...
C. N. Stewart, S. M. Leffel, S. A. Mabon
openaire +3 more sources
A modular toolset of phiC31-based fluorescent protein tagging vectors for Drosophila
Fly, 2019 The Drosophila transgenic technology and fluorescent protein fusions are powerful tools to analyze protein expression patterns, subcellular localization and protein dynamics.
Jun Luo, Pingping Shen, Jiong Chen
doaj +1 more source
Infinite average lifetime of an unstable bright state in the green fluorescent protein [PDF]
, 2005 The time evolution of the fluorescence intensity emitted by well-defined ensembles of Green Fluorescent Proteins has been studied by using a standard confocal microscope. In contrast with previous results obtained in single molecule experiments, the photo-bleaching of the ensemble is well described by a model based on Levy statistics.
arxiv +1 more source
A Diabatic Three-State Representation of Photoisomerization in the Green Fluorescent Protein Chromophore [PDF]
S. Olsen and R.H. McKenzie, The Journal of Chemical Physics, 130,
184302 (2009), 2009 We give a quantum chemical description of bridge photoisomerization reaction of green fluorescent protein (GFP) chromophores using a representation over three diabatic states. Bridge photoisomerization leads to non-radiative decay, and competes with fluorescence in these systems.
arxiv +1 more source
Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle
Microbial Cell Factories, 2023 Background Several fusion tags for separation handle have been developed, but the fused tag for simply and cheaply separating the target protein is still lacking.
Xiaomei He +6 more
doaj +1 more source
Imaging individual green fluorescent proteins [PDF]
Nature, 1997 Recent advances in fluorescence microscopy techniques have allowed the video-time imaging of single molecules of fluorescent dyes covalently bound to proteins in aqueous environments1. However, the techniques have not been exploited fully because proteins can be difficult to label, and dye modification may cause partial or complete loss of activity ...
Ronald D. Vale +2 more
openaire +3 more sources