Results 41 to 50 of about 491,462 (265)
BioTechniques, 2017 Protocol Summary Here we present an ethanol fixation protocol that allows for the direct visualization of the natural fluorescence of reporter proteins while maintaining excellent tissue histology.
Akifumi Nakagawa +5 more
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Photooxidation-guided ultrastructural identification and analysis of cells in neuronal tissue labeled with green fluorescent protein. [PDF]
PLoS ONE, 2013 The ultrastructural characterization of neuronal compartments in intact tissue labeled with green fluorescent protein (GFP) remains a frequently encountered challenge, despite work establishing photooxidation of GFP in cultured cells.
Heinz Horstmann +2 more
doaj +1 more source
Bright Green Biofluorescence in Sharks Derives from Bromo-Kynurenine Metabolism
iScience, 2019 Summary: Although in recent years there has been an increased awareness of the widespread nature of biofluorescence in the marine environment, the diversity of the molecules responsible for this luminescent phenotype has been mostly limited to green ...
Hyun Bong Park +8 more
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FEBS Letters, EarlyView.Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source
A family of GFP-like proteins with different spectral properties in lancelet
Biology Direct, 2008 Background Members of the green fluorescent protein (GFP) family share sequence similarity and the 11-stranded β-barrel fold. Fluorescence or bright coloration, observed in many members of this family, is enabled by the intrinsic properties of the ...
Mushegian Arcady +6 more
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Green fluorescent protein: A perspective [PDF]
Protein Science, 2011 AbstractA brief personal perspective is provided for green fluorescent protein (GFP), covering the period 1994–2011. The topics discussed are primarily those in which my research group has made a contribution and include structure and function of the GFP polypeptide, the mechanism of fluorescence emission, excited state protein transfer, the design of ...
openaire +2 more sources
An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots
FEBS Letters, EarlyView.This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino +7 more
wiley +1 more source
Sea as a color palette: the ecology and evolution of fluorescence
Zoological Letters, 2020 Fluorescence and luminescence are widespread optical phenomena exhibited by organisms living in terrestrial and aquatic environments. While many underlying mechanistic features have been identified and characterized at the molecular and cellular levels ...
Marie-Lyne Macel +5 more
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Green Fluorescent Protein Glows Gold [PDF]
Cell, 2008 The awarding of this year's Nobel Prize in Chemistry to Osamu Shimomura, Martin Chalfie, and Roger Tsien for their discovery and development of green fluorescent protein earns this humble jellyfish protein a place of honor in the biology research hall of fame.
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Green‐fluorescent protein mutants with altered fluorescence excitation spectra [PDF]
FEBS Letters, 1995 Using random mutagenesis and visual selection of fluorescent clones, we have isolated a T2031 and a E222G mutant of the Aequorea green‐fluorescent protein. Each mutant has one of the two fluorescence excitation bands of the wild type deleted and retains the other without a wavelength shift.
Ehrig, Torsten +2 more
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