A mant-GDP Dissociation Assay to Compare the Guanine Nucleotide Binding Preference of Small GTPases
Bio-Protocol, 2021 Small GTPases are cellular switches that are switched on when bound to GTP and switched off when bound to GDP. Different small GTPase proteins or those with mutations may bind to GTP or GDP with different relative affinities.
玉萍 tanyuping, Qingxiang Sun
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Gαs slow conformational transition upon GTP binding and a novel Gαs regulator
iScience, 2023 Summary: G proteins are major signaling partners for G protein-coupled receptors (GPCRs). Although stepwise structural changes during GPCR–G protein complex formation and guanosine diphosphate (GDP) release have been reported, no information is available
Donghoon Ahn +12 more
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Interaction of GTP‐binding proteins with calmodulin [PDF]
FEBS Letters, 1986 Two GTP‐binding proteins (Gi and Go), which were the substrates for islet‐activating protein, pertussis toxin, were purified from bovine cerebral cortical membranes. Both Gi and Go completely inhibited calmodulin‐stimulated cyclic nucleotide phosphodiesterase activity. The same concentrations of these proteins, however, had no appreciable effect on the
Asano, Tomiko +3 more
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Molecular modeling study for interaction between Bacillus subtilis Obg and Nucleotides. [PDF]
PLoS ONE, 2010 The bacterial Obg proteins (Spo0B-associated GTP-binding protein) belong to the subfamily of P-loop GTPase proteins that contain two equally and highly conserved domains, a C-terminal GTP binding domain and an N-terminal glycine-rich domain which is ...
Yuno Lee +6 more
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The speed of GTP hydrolysis determines GTP cap size and controls microtubule stability
eLife, 2020 Microtubules are cytoskeletal polymers whose function depends on their property to switch between states of growth and shrinkage. Growing microtubules are thought to be stabilized by a GTP cap at their ends.
Johanna Roostalu +5 more
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Journal of Fungi, 2022 The yeast mitochondrial transport of GTP and GDP is mediated by Ggc1p, a member of the mitochondrial carrier family. The physiological role of Ggc1p in S.
Roberta Seccia +11 more
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PH Domain-Arf G Protein Interactions Localize the Arf-GEF Steppke for Cleavage Furrow Regulation in Drosophila. [PDF]
PLoS ONE, 2015 The recruitment of GDP/GTP exchange factors (GEFs) to specific subcellular sites dictates where they activate small G proteins for the regulation of various cellular processes.
Donghoon M Lee +4 more
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Characterization of membrane-bound small GTP-binding proteins from Nicotiana tabacum [PDF]
, 1995 Haizel T, Merkle T, Turck F, Nagy F. Characterization of membrane-bound small GTP-binding proteins from Nicotiana tabacum. Plant Physiology.
Haizel, T +11 more
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Generation of specific antibodies against the rap1A, rap1B and rap2 small GTP-binding proteins. Analysis of rap and ras proteins in membranes from mammalian cells [PDF]
, 1992 Specific antibodies against rap1A and rap1B small GTP-binding proteins were generated by immunization of rabbits with peptides derived from the C-terminus of the processed proteins.
Schwaner, I. +5 more
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GTP Binding Assays in Arabidopsis thaliana
Bio-Protocol, 2013 Signaling through G proteins constitutes an ancient mechanism that functions in the transduction of extracellular signals into intracellular responses. Activation of a proper receptor by a stimuli leads to an exchange of GDP for GTP, the activation of G ...
Ana Fox, Maria Mazzella
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