A mant-GDP Dissociation Assay to Compare the Guanine Nucleotide Binding Preference of Small GTPases
Bio-Protocol, 2021 Small GTPases are cellular switches that are switched on when bound to GTP and switched off when bound to GDP. Different small GTPase proteins or those with mutations may bind to GTP or GDP with different relative affinities.
玉萍 tanyuping, Qingxiang Sun
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Nucleotide binding switches the information flow in ras GTPases. [PDF]
PLoS Computational Biology, 2011 The Ras superfamily comprises many guanine nucleotide-binding proteins (G proteins) that are essential to intracellular signal transduction. The guanine nucleotide-dependent intrinsic flexibility patterns of five G proteins were investigated in atomic ...
Francesco Raimondi +3 more
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Molecular modeling study for interaction between Bacillus subtilis Obg and Nucleotides. [PDF]
PLoS ONE, 2010 The bacterial Obg proteins (Spo0B-associated GTP-binding protein) belong to the subfamily of P-loop GTPase proteins that contain two equally and highly conserved domains, a C-terminal GTP binding domain and an N-terminal glycine-rich domain which is ...
Yuno Lee +6 more
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The speed of GTP hydrolysis determines GTP cap size and controls microtubule stability
eLife, 2020 Microtubules are cytoskeletal polymers whose function depends on their property to switch between states of growth and shrinkage. Growing microtubules are thought to be stabilized by a GTP cap at their ends.
Johanna Roostalu +5 more
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Journal of Fungi, 2022 The yeast mitochondrial transport of GTP and GDP is mediated by Ggc1p, a member of the mitochondrial carrier family. The physiological role of Ggc1p in S.
Roberta Seccia +11 more
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PH Domain-Arf G Protein Interactions Localize the Arf-GEF Steppke for Cleavage Furrow Regulation in Drosophila. [PDF]
PLoS ONE, 2015 The recruitment of GDP/GTP exchange factors (GEFs) to specific subcellular sites dictates where they activate small G proteins for the regulation of various cellular processes.
Donghoon M Lee +4 more
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Turning Platelets Off and On: Role of RhoGAPs and RhoGEFs in Platelet Activity
Frontiers in Cardiovascular Medicine, 2022 Platelet cytoskeletal reorganisation is a critical component of platelet activation and thrombus formation in haemostasis. The Rho GTPases RhoA, Rac1 and Cdc42 are the primary drivers in the dynamic reorganisation process, leading to the development of ...
Shane P. Comer, Shane P. Comer
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GTP Binding Assays in Arabidopsis thaliana
Bio-Protocol, 2013 Signaling through G proteins constitutes an ancient mechanism that functions in the transduction of extracellular signals into intracellular responses. Activation of a proper receptor by a stimuli leads to an exchange of GDP for GTP, the activation of G ...
Ana Fox, Maria Mazzella
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GTP-binding-defective ARL4D alters mitochondrial morphology and membrane potential. [PDF]
PLoS ONE, 2012 ARL4D, ARL4A, and ARL4C are closely related members of the ADP-ribosylation factor/ARF-like protein (ARF/ARL) family of GTPases. All three ARL4 proteins contain nuclear localization signals (NLSs) at their C-termini and are primarily found at the plasma ...
Chun-Chun Li +7 more
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LRRK2 kinase activity is dependent on LRRK2 GTP binding capacity but independent of LRRK2 GTP binding. [PDF]
PLoS ONE, 2011 Leucine rich repeat kinase 2 (LRRK2) is a Parkinson's disease (PD) gene that encodes a large multidomain protein including both a GTPase and a kinase domain.
Jean-Marc Taymans +7 more
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