Results 121 to 130 of about 46,713 (191)

A High‐Throughput Ancient DNA Extraction Method for Large‐Scale Sample Screening

open access: yesMolecular Ecology Resources, Volume 25, Issue 4, May 2025.
ABSTRACT Large‐scale DNA screening of palaeontological and archaeological collections remains a limiting and costly factor for ancient DNA studies. Several DNA extraction protocols are routinely used in ancient DNA laboratories and have even been automated on robotic platforms.
Alexandre Gilardet   +11 more
wiley   +1 more source

Improving Whole Biodiversity Monitoring and Discovery With Environmental DNA Metagenomics

open access: yesMolecular Ecology Resources, EarlyView.
ABSTRACT Environmental DNA (eDNA) metagenomics sequences all DNA molecules present in environmental samples and has the potential of identifying virtually any organism from which they are derived. However, due to unacceptable levels of false positives and negatives, this approach is underexplored as a tool for biodiversity monitoring across the tree of
Manuel Curto   +7 more
wiley   +1 more source

An enrichment‐based capture method from nuclear environmental DNA presents new opportunities for population genomics: A case study on the common frog

open access: yesMethods in Ecology and Evolution, EarlyView.
Abstract Population genetics is a powerful tool for studying evolutionary processes and informing conservation biology. Traditional approaches typically rely on tissue sampling, which poses challenges in aquatic environments where specimen collection is often difficult.
Stéphanie Manel   +13 more
wiley   +1 more source

Luteinizing hormone receptor knockout mouse: What has it taught us?

open access: yesAndrology, EarlyView.
Abstract Luteinizing hormone (LH), along with its agonist choriongonadotropin (hCG) in humans, is the key hormone responsible for the tropic regulation of the gonadal function. LH and hCG act through their cognate receptor, the luteinizing hormone/choriongonadotropin receptor (LHCGR; more appropriately LHR in rodents lacking CG), located in the testis ...
Ilpo T. Huhtaniemi
wiley   +1 more source

Inflammation and response to bacterial infection as potential drivers of equine odontoclastic tooth resorption and hypercementosis: A proteomics insight

open access: yesEquine Veterinary Journal, EarlyView.
Abstract Background Equine dental diseases significantly impact a horse's overall health, performance and quality of life. They can result in secondary infections and digestive disturbances, potentially leading to colic. A recently described disease affecting the incisors of horses is equine odontoclastic tooth resorption and hypercementosis (EOTRH ...
Anders Jensen   +9 more
wiley   +1 more source

The complete absence of cytoplasmic γ‐actin results in no discernible phenotype in mice or primary fibroblasts

open access: yesThe FEBS Journal, EarlyView.
Mice and mouse embryonic fibroblasts homozygous for an Actg1 allele edited to translate β‐actin instead of γ‐actin (Actg1‐coding beta) are devoid of γ‐actin protein. We demonstrate that these Actg1c‐b/c‐b mice present with no measurable phenotype in survival, body mass, activity, muscle contractility, or auditory function.
Lauren J. Sundby   +7 more
wiley   +1 more source

Scat DNA Applications for Low‐Density Carnivore Survey: Techniques, Efficiency and Future Directions

open access: yesMammal Review, EarlyView.
We reviewed 338 studies encompassing mostly Felidae, Canidae and Mustelidae species throughout the world, confirming scat DNA as a reliable genetic source for many applications. The findings here offer guidance for future studies on low‐density carnivores, helping to design cost‐effective research and improving data quality for conservation efforts ...
Marina Elisa de Oliveira   +2 more
wiley   +1 more source

Heterologous Surface Display Reveals Conserved Complement Inhibition and Functional Diversification of Borrelia burgdorferi Elp Proteins

open access: yesMolecular Microbiology, EarlyView.
A novel approach using the Pseudomonas syringae ice nucleation protein (INP) system to present Borrelia burgdorferi lipoproteins on the surface of Escherichia coli reveals conservation of complement inhibitory functions among uncharacterized OspE/F‐like leader peptide (Elp) proteins.
Nathan Hill   +10 more
wiley   +1 more source

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