Results 51 to 60 of about 283,665 (235)

Plasma DNMT1 Activity for Assessing Tumor Burden and Predicting Neoadjuvant Therapy Response in Breast Cancer

Advanced Science, EarlyView.
In this work, a detection method is presented for the activity of plasma DNA methyltransferase 1 (DNMT1), namely DNMT1 Identification by Variable Activity (DIVA). DIVA can detect plasma DNMT1 at levels as low as 10−7 U mL−1, also it successfully evaluated tumor burden and predicted the neoadjuvant therapy responses of breast cancer patients.
Yingran Wang, Guozhi Zhang, Zhizhao Zhang, Mengsi Zhang, Jiao Chen, Ke Wang, Lu Liu, Jing Bao, Ming Chen, Xiaowei Qi, Mingxuan Gao   +10 more
wiley   +1 more source

Global deformation facilitates flipping of damaged 8-oxo-guanine and guanine in DNA [PDF]

Nucleic Acids Research, 2016
Oxidation of guanine (Gua) to form 7,8-dihydro-8-oxoguanine (8oxoG) is a frequent mutagenic DNA lesion. DNA repair glycosylases such as the bacterial MutM can effciently recognize and eliminate the 8oxoG damage by base excision. The base excision requires a 8oxoG looping out (flipping) from an intrahelical base paired to an extrahelical state where the
Martin Zacharias, Giuseppe La Rosa
openaire   +4 more sources

Molecular mechanism of Gαi activation by non-GPCR proteins with a Gα-Binding and Activating motif [PDF]

, 2017
Heterotrimeric G proteins are quintessential signalling switches activated by nucleotide exchange on Gα. Although activation is predominantly carried out by G-protein-coupled receptors (GPCRs), non-receptor guanine-nucleotide exchange factors (GEFs) have
Baillie, George S., Blanco, Francisco J., Blanco-Canosa, Juan B., Cerione, Richard A., de la Cruz-Morcillo, Miguel Angel, DiGiacomo, Vincent, Garcia-Marcos, Mikel, Ibáñez de Opakua, Alain, Leyme, Anthony, Marivin, Arthur, Merino, Nekane, Nguyen, Lien T., Parag-Sharma, Kshitij, Ramachandran, Sekar, Villate, Maider   +14 more
core   +1 more source

Fn14 Controls the SIRT2‐Mediated Deacetylation of Slug to Inhibit the Metastasis of Epithelial Ovarian Cancer

Advanced Science, EarlyView.
It is reported that Fn14 inhibited EOC metastasis by regulating Slug‐mediated EMT. Furthermore, Fn14 altered the subcellular localization of SIRT2 by interacting with SIRT2, leading to reduced SIRT2 shuttling into the nucleus and subsequently promoting the acetylated degradation of Slug.
Anyue Wu, Shengze Li, Chunyang Feng, Ruiju He, Ruolan Wu, Zhijun Hu, Jinhua Huang, Wenjing Wang, Lei Huang, Lihua Qiu   +9 more
wiley   +1 more source

Exocytosis from permeabilized bovine adrenal chromaffin cells is differently modulated by guanosine 5'-[gamma-thio]triphosphate and guanosine 5'-[beta gamma-imido]triphosphate [PDF]

, 1992
1. In bovine adrenal chromaffin cells made permeable either to molecules less than or equal to 3 kDa with alphatoxin or to proteins less than or equal to 150 kDa with streptolysin O, the GTP analogues guanosine 5'-[beta gamma-imido]triphosphate (p[NH]ppG)
Ahnert-Hilger, G., Gratzl, Manfred, Rosenthal, W., Spicher, K., Stecher, B., Wegenhorst, U.   +5 more
core  

Clerocidin selectively modifies the gyrase-DNA gate to induce irreversible and reversible DNA damage [PDF]

, 2008
Clerocidin (CL), a microbial diterpenoid, reacts with DNA via its epoxide group and stimulates DNA cleavage by type II DNA topoisomerases. The molecular basis of CL action is poorly understood.
Binaschi, Borgnetto, Brown, Cambau, Champoux, Corbett, Corbett, Ferrero, Fisher, Gatto, Higgins, Kawada, L. M. Fisher, M. Dias, M. Palumbo, Marians, Maxam, McCullough, Mizuuchi, Morrison, Pan, Pan, Pan, Pan, Pan, Peden, Pozzi, Richter, Richter, Richter, Shea, X. S. Pan, Yague, Yoshida, Zechiedrich   +34 more
core   +1 more source

Cartilage‐Penetrating Framework Nucleic Acid Nanoparticles Ameliorate Osteoarthritis by Promoting Drug Delivery and Chondrocyte Uptake

Advanced Science, EarlyView.
Based on the poor cartilage penetration of tFNA, a novel tFNA‐2WL with excellent cartilage permeability and perfect chondrocyte endocytosis properties is designed and fabricated. As a result, tFNA‐2WL has a nearly seven‐fold increase in the penetration depth into the cartilage, with the same excellent cell entry properties as tFNA.
Kui Huang, Qiumei Li, Huixuan Lin, Qian Shen, Yaping Wu, Taoran Tian, Chuan Ma, Sirong Shi, Jingang Xiao, Yunfeng Lin   +9 more
wiley   +1 more source

Ribozyme‐Catalyzed Site‐Specific Labeling of RNA Using O6‐alkylguanine SNAP‐Tag Substrates

Angewandte Chemie, EarlyView.
SNAPR is a ribozyme that repurposes O6‐benzylguanine SNAP‐tag substrates for site‐specific labeling of RNA with bioorthogonal alkyne and azide functional groups, fluorophores or photocrosslinkers. The N1A‐alkylated RNA products were converted to N6A‐modified RNA by Dimroth rearrangement.
Manisha B. Walunj, Carolin P. M. Scheitl, Tina Jungnickel, Claudia Höbartner   +3 more
wiley   +2 more sources

Back-electron transfer suppresses the periodic length dependence of DNA-mediated charge transport across adenine tracts [PDF]

, 2008
DNA-mediated charge transport (CT) is exquisitely sensitive to the integrity of the bridging π-stack and is characterized by a shallow distance dependence. These properties are obscured by poor coupling between the donor/acceptor pair and the DNA bridge,
Augustyn, Katherine E., Barton, Jacqueline K., Davis, Molly L., Genereux, Joseph C., Shao, Fangwei   +4 more
core   +2 more sources

In Situ Assembly of Fluorogenic RNA for Screening Natural Anti‐Liver Fibrosis Products via Dynamic Visualization of COL1A1 mRNA

Advanced Science, EarlyView.
This study introduces an in situ assembly of fluorogenic RNA approach for screening natural anti‐liver fibrosis compounds and dynamic visualization of endogenous COL1A1 mRNA in live cells. It offers a simple, cost‐effective approach of visualizing RNA dynamics and conducting drug screening in live cells, presenting a significant potential for the ...
Rui Bai, Li‐Zeng Zhu, Changfa Shao, Zheng Yin, Qun Liu, Yu Gu, Bin Liu   +6 more
wiley   +1 more source