Results 91 to 100 of about 52,163 (306)

Nucleic Acid Therapeutics for “Undruggable” Cancer Targets: Mechanisms, Challenges, and Prospects

Advanced Science, EarlyView.
Nucleic acid therapeutics bypass the structural limitations of conventional drugs by targeting mRNA rather than proteins. This review examines how antisense oligonucleotides, siRNAs, miRNAs, aptamers, and mRNA vaccines intervene against historically undruggable oncoproteins including Ras, MYC, and p53, highlighting mechanistic advances, delivery ...
Feng Xu, Ke Wang, Kaizhong Lu, Tianlun Hou, Yuan Chen, Xian Wang, Hongchuan Jin   +6 more
wiley   +1 more source

Novel Mutation at Cys225 in GNAO1‐Associated Developmental and Epileptic Encephalopathies: Clinical, Molecular, and Pharmacological Profiling of Case Studies

MedComm
GNAO1‐associated disorders have a large spectrum of neurological symptoms, from early‐onset developmental and epileptic encephalopathies (DEE) to late‐onset movement disorders. First reported in 2013 and now identified in around 400 cases worldwide, this
Yonika A. Larasati, Gonzalo P. Solis, Alexey Koval, Marie‐Céline François‐Heude, Julie Piarroux, Agathe Roubertie, Ruihan Yang, Ying Zhang, Dezhi Cao, Christian M. Korff, Vladimir L. Katanaev   +10 more
doaj   +1 more source

Sequencing and Analysis of the Genome of Propionibacterium freudenreichii T82 Strain: Importance for Industry

Biomolecules, 2020
The genome of Propionibacterium freudenreichii ssp. freudenreichii T82, which has a chromosome containing 2,585,340 nucleotides with 67.3% GC content (guanine-cytosine content), is described in this paper. The total number of genes is 2308, of which 2260
Kamil Piwowarek, Edyta Lipińska, Elżbieta Hać-Szymańczuk, Marek Kieliszek, Anna Maria Kot   +4 more
doaj   +1 more source

Heat Shock Protein 90: From Molecular Chaperone Function to Therapeutic Targeting in Malignancies

Advanced Science, EarlyView.
In this review, an integrated conceptual framework linking HSP90's molecular chaperone functions to its pathological roles in cancer is proposed. HSP90 serves as a central node that integrates oncogenic signaling, buffers proteotoxic stress, maintains cancer stem cell plasticity, and shapes tumor‐immune interactions, all of which converge to drive ...
Beibei Zhang, Xinxin Zou, Jiansong Zhou, Qinmiao Sun, Dahua Chen   +4 more
wiley   +1 more source

Bacterial Signaling Nucleotides Inhibit Yeast Cell Growth by Impacting Mitochondrial and Other Specifically Eukaryotic Functions

mBio, 2017
We have engineered Saccharomyces cerevisiae to inducibly synthesize the prokaryotic signaling nucleotides cyclic di-GMP (cdiGMP), cdiAMP, and ppGpp in order to characterize the range of effects these nucleotides exert on eukaryotic cell function during ...
Andy Hesketh, Marta Vergnano, Chris Wan, Stephen G. Oliver   +3 more
doaj   +1 more source

Guanine nucleotide determination in extracts of wheat embryo

FEBS Letters, 1976
A number of recent studies have been concerned with the relationship between the rates of RNA and protein synthesis and the cellular levels of nucleotide triphosphates [l-4] . Prerequisite for such studies are methods for accurate nucleotide analysis. While in the case of the adenine nucleotides a number of satisfactory procedures have been described ...
Cheung, Chi P., Marcus, Abraham
openaire   +2 more sources

A General Enzymatic Strategy for Site‐Specific Incorporation of Modified Genetic Building Blocks Into DNA

Advanced Science, EarlyView.
Eco‐friendly enzymatic synthesis of high‐fidelity, site‐specifically modified DNA using 3′‐blocked nucleotides for full synthetic control and next‐generation sequencing compatibility. ABSTRACT Methods for site‐specific incorporation of modified DNA building blocks remain limited.
Raveena Raveena, Bhavana Ramadas, Sidney Becker   +2 more
wiley   +1 more source

Rescue of high-specificity Cas9 variants using sgRNAs with matched 5’ nucleotides

Genome Biology, 2017
We report that engineered Cas9 variants with improved specificity—eCas9-1.1 and Cas9-HF1—are often poorly active in human cells, when complexed with single guide RNAs (sgRNAs) with a mismatch at the 5’ terminus, relative to target DNA sequences.
Sojung Kim, Taegeun Bae, Jaewoong Hwang, Jin-Soo Kim   +3 more
doaj   +1 more source

Guanine nucleotides modulate the ligand binding properties of cell surface folate receptors in Dictyostelium discoideum

, 1985
Dictyostelium discoideum cells show 2 distinct classes of cell surface binding sites for folates. One type is non-specific, i.e., binds folic acid (FA), 2-deaminofolic acid (DAFA), and methotrexate (MTX) with similar affinity (K0.5 ⋍ 140 nM).
René J.W. De Wit, De Wit, René J.W., Bulgakov, Roman, Roman Bulgakov   +3 more
core   +1 more source

A Conserved DT2‐bZIP66‐NF‐YC4 Regulatory Module Confers Drought Tolerance in Rice and Arabidopsis

Advanced Science, EarlyView.
This study identifies a conserved tripartiteDT2–bZIP66–NF‐YC4 transcriptional module that enhances rice drought tolerance by activating stress‐responsive genes. This regulatory complex is functionally conserved across cereals and Arabidopsis, providing promising targets for engineering drought‐resilient crops. ABSTRACT Drought stress severely restricts
Jun Shen, Yuchen Li, Liang Zhang, Xiaosong Ma, Chenyin Peng, Chunting Yuan, Yu Wu, Kaiyu Zhao, Sheng Zhao, Jiazhuo Guo, Run Li, Ziqian Yan, Shule Ren, Yige Han, Xin Lin, Xinyue Li, Ming Li, Pingfang Yang, Shiyong Song   +18 more
wiley   +1 more source