Results 101 to 110 of about 150,931 (301)
Rescue of high-specificity Cas9 variants using sgRNAs with matched 5’ nucleotides
Genome Biology, 2017 We report that engineered Cas9 variants with improved specificity—eCas9-1.1 and Cas9-HF1—are often poorly active in human cells, when complexed with single guide RNAs (sgRNAs) with a mismatch at the 5’ terminus, relative to target DNA sequences.
Sojung Kim +3 more
doaj +1 more source
Estudo de Agentes Neuroprotetores em um Modelo de Isquemia Cerebral in vitro [PDF]
, 2015 TCC(graduação) - Universidade Federal de Santa Catarina. Centro de Ciências Biológicas. Biologia.A isquemia cerebral consiste na interrupção do fluxo sanguíneo cerebral que resulta em uma severa degeneração das células neurais e conseqüente perda das ...
Martins, Wagner Carbolin
core
, 1981 These studies examine the interactions of Ca2+, Mg2+, and guanine nucleotides with PTH binding to renal receptors and activation of adenylate cyclase in a purified preparation of basolateral renal cortical membranes.
K. J. Martin, E. Bellorin-Font
core +1 more source
Simultaneous quantification of 12 different nucleotides and nucleosides released from renal epithelium and in human urine samples using ion-pair reversed-phase HPLC [PDF]
, 2012 Nucleotides and nucleosides are not only involved in cellular metabolism but also act extracellularly via P1 and P2 receptors, to elicit a wide variety of physiological and pathophysiological responses through paracrine and autocrine signalling pathways.
Claire M. Peppiatt-Wildman +18 more
core +1 more source
Jurnal Veteriner, 2017 Tree frog (Polypedates celebensis) is an endemic amphibi found in Southeast Sulawesi. Database ofthis species, in particular genetic databases is still very limited.
Suriana Suriana, Nasaruddin Nasaruddin
doaj
Quantum‐Level Influences on Enzyme Reactions and Their Ripple Effects on Biological Phenomena
Advanced Physics Research, EarlyView.Because living organisms are ultimately composed of atoms and molecules, quantum‐mechanical effects are expected to contribute to biological phenomena. Quantum biology seeks to elucidate life processes from a quantum‐mechanical perspective. This review discusses how quantum‐level effects, including quantum tunneling, may propagate from enzymatic proton‐
Takeshi Yasuda
wiley +1 more source
Communications BiologyTransglutaminase 2 (TG2) is a GTP-binding, protein-crosslinking enzyme that has been investigated as a therapeutic target for Celiac disease, neurological disorders, and aggressive cancers.
Cody Aplin +7 more
doaj +1 more source
Interplay of 'induced fit' and preorganization in the ligand induced folding of the aptamer domain of the guanine binding riboswitch [PDF]
, 2006 Riboswitches are highly structured elements in the 50-untranslated regions (50-UTRs) of messenger RNA that control gene expression by specifically binding to small metabolite molecules. They consist of an aptamer domain responsible for ligand binding and
Wöhnert, Jens +5 more
core +1 more source
British Journal of Clinical Pharmacology, EarlyView.While the event‐free survival (EFS) of children treated for acute lymphoblastic leukaemia (ALL) has improved greatly in the last decades, the EFS for patients diagnosed with ALL before the age of one is still under 50%. This outcome further decreases when infants have a rearrangement in the gene encoding histone‐lysine N‐methyltransferase 2A (KMT2A ...
Tirsa de Kluis +5 more
wiley +1 more source
Regulation of a liver plasma membrane phosphoinositide phosphodiesterase by guanine nucleotides and calcium [PDF]
, 1985 Rat liver plasma membranes are enriched in a Ca2+-dependent phosphodiesterase active on phosphatidylinositol 4,5-P2 and phosphatidylinositol 4-P, but not phosphatidylinositol.
Exton, John H. +7 more
core +1 more source