Results 81 to 90 of about 99,476 (367)

Bacillus anthracis edema factor substrate specificity: evidence for new modes of action [PDF]

, 2012
Since the isolation of Bacillus anthracis exotoxins in the 1960s, the detrimental activity of edema factor (EF) was considered as adenylyl cyclase activity only.
Agrawal, Altaweel, Basler, Bauche, Bell, Benson, Beste, Bock, Bond, Boyd, Bradley, Brown, Burhenne, Carbonetti, Carbonetti, Carson-Walter, Casteel, Christensen, Christensen, Confer, Copsel, Crawford, Crowe, Cunningham, Dalkas, Defer, Deuquet, Ding, Drum, Duesbery, Feld, Firoved, Friedlander, Fu, Fukuhara, Gaion, Gille, Gordon, Göttle, Hammerschmidt, He, Helfman, Hong, Ivarsson, Jernigan, Johnson, Katayama, Katayama, Kaupp, Kintzer, Klimpel, Laine, Larabee, Larabee, Leppla, Liu, Martin Göttle, McDonough, Milne, Mogridge, Mogridge, Newton, Newton, Newton, Newton, Newton, Oncu, O´Brien, Park, Petosa, Pezard, Postea, Puhar, Roland Seifert, Santelli, Scobie, Scobie, Scorpio, Shapiro, Shen, Shirshev, Soelaiman, Stefan Dove, Sun, Tamayo, Taylor, Taylor, Tesmer, Thomas, Tournier, Vitale, Vitale, Vojtova, Yeager, Zong   +94 more
core   +2 more sources

Compartmentation of guanine nucleotide precursors for DNA synthesis [PDF]

Biochemical Journal, 1986
We have studied the kinetics of guanine incorporation into DNA in mouse T-lymphoma (S-49) mutant cells [PNPase (purine-nucleoside phosphorylase)- and HGPRTase (hypoxanthine: guanine phosphoribosyltransferase)-deficient] that are incapable of converting dGuo (deoxyguanosine) to Gua (guanine) ribonucleotides. Of the two possible pathways for an exogenous
Wolfgang Sadee, Binh Trong Nguyen
openaire   +3 more sources

Interaction of Oligonucleotides with Gold Nanoparticles: Factors Beyond Electrostatic and Van‐Der Waals Forces

Advanced Materials Interfaces, EarlyView.
The formation of stable clusters of gold nanoparticles with oligonucleotides at different solution conditions is explored. DNA base homogeneity and strand directionality influence the binding with gold nanoparticles leading to interesting clustering behavior that can be relevant for SERS applications.
Shaila Thakur, Nicola Cavallini, Debora Ferrari, Laura Fabris   +3 more
wiley   +1 more source

Genetic predisposition to porto‐sinusoidal vascular disorder: A functional genomic‐based, multigenerational family study

Hepatology, EarlyView., 2022
A deleterious variant of FCHSD1 results in mTOR pathway overactivation and may cause porto‐sinusoidal vascular disorder (PSVD). The pedigree of the family demonstrated an autosomal dominant disease with variable expressivity. Whole‐genome sequencing and Sanger sequencing both validated the existence of the FCHSD1 variant and the heterozygosity of c ...
Jingxuan Shan, André Megarbane, Aziz Chouchane, Deepak Karthik, Ramzi Temanni, Atilio Reyes Romero, Huiying Hua, Chun Pan, Xixi Chen, Murugan Subramanian, Chadi Saad, Hamdi Mbarek, Cybel Mehawej, Eliane Chouery, Sirin W. Abuaqel, Alexander Dömling, Sami Remadi, Cesar Yaghi, Pu Li, Lotfi Chouchane   +19 more
wiley   +1 more source

Live-cell analysis of IMPDH protein levels during yeast colony growth provides insights into the regulation of GTP synthesis

mBio
The purine nucleotides ATP and GTP are made from the common precursor inosine monophosphate (IMP). Maintaining the correct balance of these nucleotides for optimal cell growth is controlled in part by the enzyme IMP dehydrogenase (IMPDH), which catalyzes
Erica L. Shand, Kieran Sweeney, Kaitlin E. Sundling, Megan N. McClean, David A. Brow   +4 more
doaj   +1 more source

Precisely Monomeric Linear RNAs of Viroids Belonging to Pospiviroid and Hostuviroid Genera Are Infectious Regardless of Transcription Initiation Site and 5′-Terminal Structure

Cells, 2021
Infectious dimeric RNA transcripts are a powerful tool for reverse genetic analyses in viroid studies. However, the construction of dimeric cDNA clones is laborious and time consuming, especially in mutational analyses by in vitro mutagenesis.
Tatsuji Hataya, Takashi Naoi
doaj   +1 more source

Small RNA Toxin‐Assisted Evolution of GC‐Preferred ErCas12a for Enhanced Genome Targeting Range

Advanced Science, EarlyView.
ErCas12a is engineered to target GC‐rich PAMs using a small RNA toxin‐aided positive screening system. The resulting variant, enErCas12a, exhibits an expanded PAM profile and facilitates efficient gene editing in both bacterial and mammalian cells, while preserving high targeting specificity for both canonical and non‐canonical PAM targets.
Zehua Chen, Junyuan Xue, Ziying Wang, Jinyuan Sun, Yinglu Cui, Tong Zhu, Huaiyi Yang, Ming Li, Bian Wu   +8 more
wiley   +1 more source

Janus kinase 2 inhibition by pacritinib as potential therapeutic target for liver fibrosis

Hepatology, EarlyView., 2022
Diagram of the activation of the profibrotic and procontractile Janus kinase 2 (JAK2)/Ras homolog family member A/Rho‐kinase pathway and the inhibition of phosphorylated JAK2 by pacritinib to inhibit hepatic stellate cell activity. Abstract Background and Aims Janus kinase 2 (JAK2) signaling is increased in human and experimental liver fibrosis with ...
Sandra Torres, Cristina Ortiz, Nadine Bachtler, Wenyi Gu, Leon D. Grünewald, Nico Kraus, Robert Schierwagen, Christoph Hieber, Caroline Meier, Olaf Tyc, Maximilian Joseph Brol, Frank Erhard Uschner, Bart Nijmeijer, Christoph Welsch, Marie‐Luise Berres, Carmen Garcia‐Ruiz, Jose Carlos Fernandez‐Checa, Christian Trautwein, Thomas J. Vogl, Stefan Zeuzem, Jonel Trebicka, Sabine Klein   +21 more
wiley   +1 more source

Fn14 Controls the SIRT2‐Mediated Deacetylation of Slug to Inhibit the Metastasis of Epithelial Ovarian Cancer

Advanced Science, EarlyView.
It is reported that Fn14 inhibited EOC metastasis by regulating Slug‐mediated EMT. Furthermore, Fn14 altered the subcellular localization of SIRT2 by interacting with SIRT2, leading to reduced SIRT2 shuttling into the nucleus and subsequently promoting the acetylated degradation of Slug.
Anyue Wu, Shengze Li, Chunyang Feng, Ruiju He, Ruolan Wu, Zhijun Hu, Jinhua Huang, Wenjing Wang, Lei Huang, Lihua Qiu   +9 more
wiley   +1 more source

Intermolecular Enzymatic Encoding of Nucleic Acid, Steroid Complexes: A New Theory on the Chemical Origin of Life Based on Evidence of Structural Symmetry [PDF]

arXiv, 2020
The origin of life is one of the greatest mysteries. The mechanism for the synthesis of DNA is synonymous with the chemical origin of life, and theories have been developed along many lines of reasoning, but resolving all requirements remains a challenge, such as defining an objective path to produce sequences of encoded nucleotides paired as adenine ...
arxiv