Bacterial alarmone (p)ppGpp mediates the pathogenicity of Clavibacter michiganensis via a dual mechanism that affects both enzyme production and the Tat secretion system [PDF]
mSystemsThe gram-positive bacterium Clavibacter michiganensis (Cm) causes bacterial canker of tomato. The infection process of Cm is of considerable interest, and the role of the alarmone guanosine tetraphosphate or pentaphosphate [(p)ppGpp], a global regulator,
Xiaoli Xu +7 more
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Guanosine pentaphosphate and guanosine tetraphosphate accumulation and induction of Myxococcus xanthus fruiting body development [PDF]
Journal of Bacteriology, 1980 Development of multicellular fruiting bodies of Myxococcus xanthus can be induced by limitation of any of a number of different classes of amino acids. Investigated were amino acids that wild-type strains of M. xanthus are unable to synthesize (isoleucine, leucine, and valine), can synthesize at a low rate (phenylalanine), or can normally synthesize at
Colin Manoil, D Kaiser
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Accumulation of Guanosine Tetraphosphate in T7 Bacteriophage-Infected Escherichia coli [PDF]
Journal of Bacteriology, 1973 Accumulation of guanosine tetraphosphate (ppGpp) in Escherichia coli strain AS19 valS , carrying a temperature-sensitive valyl-transfer ribonucleic acid synthetase and infected with bacteriophage T7, was studied. Valine starvation was achieved by culturing this strain at 42 C.
James D. Friesen, Niels P. Fiil
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Improved plant biomass production under low nitrogen conditions through conditional accumulation of the second messenger, guanosine tetraphosphate, in chloroplasts and mitochondria [PDF]
Frontiers in Plant ScienceTo enhance plant biomass production under low nitrogen conditions, we employed a method to artificially and temporarily accumulate the bacterial second messenger, guanosine tetraphosphate (ppGpp), to modify plastidial or mitochondrial metabolism ...
Mina Goto +5 more
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Synthesis and turnover of basal level guanosine tetraphosphate in Escherichia coli.
Journal of Biological Chemistry, 1975 Cultures of escherichia coli growing exponentially in Trisacetate medium were subjected to nutritional shift-up and the pool size of guanosine 5'-3'-diphosphate-3'diphosphate (ppGpp) as well as the rates of protein synthesis and net RNA synthesis were determined.
James D. Friesen +2 more
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Accumulation of guanosine tetraphosphate and guanosine pentaphosphate in Myxococcus xanthus during starvation and myxospore formation [PDF]
Journal of Bacteriology, 1980 Cultures of Myxococcus xanthus develop multicellular fruiting bodies when starved for carbon and nitrogen sources on an agar surface. Under these conditions of severe starvation, cultures rapidly accumulated a compound identified as guanosine tetraphosphate by chromatographic migration of the compound and of its major acid and alkali breakdown products.
Colin Manoil, D Kaiser
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Maintenance of cytoplasmic and membrane densities shapes cellular geometry in Escherichia coli [PDF]
Nature CommunicationsMicrobes precisely control their composition and geometry across diverse growth conditions, yet the mechanisms coordinating these processes remain unclear.
Griffin Chure +4 more
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Effects of Guanosine Tetraphosphate, Guanosine Pentaphosphate, and β-γ Methylenyl-Guanosine Pentaphosphate on Gene Expression of Escherichia coli In Vitro [PDF]
Proceedings of the National Academy of Sciences, 1974 The effects of guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp), both produced by E. coli , were measured on the activities of several genes in a cell-free system. Gene activity is measured as gene-directed synthesis of biochemically competent protein or transfer RNA.
Huey‐Lang Yang +4 more
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Control of rRNA and tRNA syntheses in Escherichia coli by guanosine tetraphosphate [PDF]
Journal of Bacteriology, 1982 The expression of stable RNA (rRNA and tRNA) genes and the concentration of guanosine tetraphosphate (ppGpp) were measured in an isogenic pair of relA+ and relA derivatives of Escherichia coli B/r. The cells were either growing exponentially at different rates or subject to amino acid starvation when they were measured.
John Ryals, Robert Little, H. J. Bremer
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Regulation of Escherichia coli aspartate transcarbamylase synthesis by guanosine tetraphosphate and pyrimidine ribonucleoside triphosphates [PDF]
Journal of Bacteriology, 1983 The effects of guanosine tetraphosphate (ppGpp) and pyrimidine ribonucleoside triphosphates on Escherichia coli aspartate transcarbamylase (ATCase) synthesis were examined. To determine the effect of ppGpp, a stringent (relA+) and relaxed (relA) isogenic pair of E. coli K-12 strains was starved for isoleucine, and the residual rate of synthesis of this
Charles L. Turnbough
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