Results 81 to 90 of about 4,703 (162)

Exogenous fatty acids inhibit fatty acid synthesis by competing with endogenously generated substrates for phospholipid synthesis in Escherichia coli

FEBS Letters, Volume 599, Issue 5, Page 667-681, March 2025.
Bacteria can absorb external fatty acids to build their membranes. Working in E. coli, we show that external fatty acids inhibit internal fatty acid production through competition for membrane synthesis. This rapid negative feedback helps bacteria conserve resources and preserve membrane properties. We predict similar mechanisms exist in many bacteria.
Stefan Pieter Hendrik van den Berg, Adja Zoumaro‐Djayoon, Flora Yang, Gregory Bokinsky   +3 more
wiley   +1 more source

The Mysterious World of Non‐Canonical Caps – What We Know and Why We Need New Sequencing Techniques

ChemBioChem, Volume 26, Issue 3, February 1, 2025.
This review describes sophisticated methodologies such as LC–MS that enabled the identification of 5’ non‐canonical RNA caps and also surveys so far developed sequencing methods, evaluating their advantages and disadvantages. It emphasizes the need for developing new, reliable sequencing techniques to identify RNA types with non‐canonical caps enabling
Flaminia Mancini, Hana Cahova
wiley   +1 more source

Distinguishing Crohn’s Disease From Non–Crohn’s Ileitis: A Gut Microbiome–Based Approach

Advanced Gut &Microbiome Research, Volume 2025, Issue 1, 2025.
Background: The differential diagnosis between symptomatic non–Crohn’s ileitis (NCI) and Crohn’s disease (CD) has been a long‐standing dilemma with similar clinical, endoscopic, and histopathology findings. We aimed to conduct a pilot study to compare the fecal microbiome between NCI and CD.
Nalini Raghunathan, Varun Joshi, Dhanush Mekala, Rajendra Patel, Shubhankar Godbole, Rupjyoti Talukdar, Duvvur Nageshwar Reddy, Rupa Banerjee, Jian Wu   +8 more
wiley   +1 more source

The contribution of mRNA targeting to spatial protein localization in bacteria

The FEBS Journal, Volume 291, Issue 21, Page 4639-4659, November 2024.
mRNA‐based targeting serves as a back‐up strategy for the canonical signal sequence‐based protein targeting in bacteria. While signal sequence‐based targeting depends on dedicated protein‐targeting factors, mRNAs encoding inner membrane proteins can bind directly to protein translocases.
Wenkang Shang, Elisabeth Lichtenberg, Andreea Mihaela Mlesnita, Annegret Wilde, Hans‐Georg Koch   +4 more
wiley   +1 more source

Adaptation of a fluoroquinolone-sensitive Shigella sonnei to norfloxacin exposure

Royal Society Open Science
Shigella causes shigellosis that requires antibiotic treatment in severe cases. Sublethal antibiotic concentrations can promote resistance, but their effect on antibiotic-sensitive bacteria before resistance development is unclear.
Bao Chi Wong, Soffi Kei Kei Law, Muhammad Zarul Hanifah Md Zoqratt, Qasim Ayub, Hock Siew Tan   +4 more
doaj   +1 more source

Antimicrobial efficacy of DJK‐5 peptide in combination with EDTA against biofilms in dentinal tubules: Primary irrigation, recovery and re‐irrigation

International Endodontic Journal, Volume 57, Issue 9, Page 1343-1359, September 2024.
Abstract Aim To investigate the dynamic recovery of biofilms within dentinal tubules after primary irrigation with different protocols, and to evaluate the efficacy of various re‐irrigation protocols on recovered biofilm, considering factors such as smear layer, nutrient conditions, and primary irrigants.
Mohamed Amhmed, He Liu, Lari Häkkinen, Markus Haapasalo, Ya Shen   +4 more
wiley   +1 more source

Broadening the Substrate Scope of a Polyphosphate Kinase for Canonical and Non‐Canonical Nucleotides

ChemCatChem, Volume 16, Issue 15, August 12, 2024.
In this study, we investigate the substrate spectrum of a polyphosphate kinase for the conversion of non‐canonical nucleotides. Guided by the structure, we created an enzyme variant with enhanced substrate scope for a broad variety of canonical and non‐canonical purine nucleotides. Abstract Polyphosphate kinases (PPKs) are valuable biocatalysts for ATP
Toni Querengässer, Jessica Wenzlaff, Christoph Loderer   +2 more
wiley   +1 more source

The excitation cascade of Limulus ventral photoreceptors: guanylate cyclase as the link between InsP₃-mediated Ca²⁺ release and the opening of cGMP-gated channels

BMC Neuroscience, 2004
Background Early stages in the excitation cascade of Limulus photoreceptors are mediated by activation of Gq by rhodopsin, generation of inositol-1,4,5-trisphosphate by phospholipase-C and the release of Ca2+.
Lisman John E, Richard Edwin A, Garger Alexander V   +2 more
doaj   +1 more source

Phages produce persisters

Microbial Biotechnology, Volume 17, Issue 8, August 2024.
Bacterial infections are predicted to be the main cause of death by 2050, and persister cells often form during periods of lethal stress. Here, we show phage infections generate persister cells, and these persisters may undermine phage therapy. Abstract Arguably, the greatest threat to bacteria is phages.
Laura Fernández‐García, Joy Kirigo, Daniel Huelgas‐Méndez, Michael J. Benedik, María Tomás, Rodolfo García‐Contreras, Thomas K. Wood   +6 more
wiley   +1 more source

The Stringent Stress Response Controls Proteases and Global Regulators under Optimal Growth Conditions in Pseudomonas aeruginosa

mSystems, 2020
The bacterial stringent stress response, mediated by the signaling molecule guanosine tetraphosphate, ppGpp, has recently gained attention as being important during normal cellular growth and as a potential new therapeutic target, which warrants detailed
Daniel Pletzer, Travis M. Blimkie, Heidi Wolfmeier, Yicong Li, Arjun Baghela, Amy H. Y. Lee, Reza Falsafi, Robert E. W. Hancock   +7 more
doaj   +1 more source