Short RNA guides cleavage by eukaryotic RNase III. [PDF]
PLoS ONE, 2007 In eukaryotes, short RNAs guide a variety of enzymatic activities that range from RNA editing to translation repression. It is hypothesized that pre-existing proteins evolved to bind and use guide RNA during evolution.
Bruno Lamontagne, Sherif Abou Elela
doaj +6 more sources
RNA Interference by Single- and Double-stranded siRNA With a DNA Extension Containing a 3′ Nuclease-resistant Mini-hairpin Structure [PDF]
Molecular Therapy: Nucleic Acids, 2014 Selective gene silencing by RNA interference (RNAi) involves double-stranded small interfering RNA (ds siRNA) composed of single-stranded (ss) guide and passenger RNAs.
Simon J Allison, Jo Milner
doaj +8 more sources
Encoding folding paths of RNA switches [PDF]
arXiv, 2006 RNA co-transcriptional folding has long been suspected to play an active role in helping proper native folding of ribozymes and structured regulatory motifs in mRNA untranslated regions. Yet, the underlying mechanisms and coding requirements for efficient co-transcriptional folding remain unclear.
Harlepp, S. +3 more
arxiv +5 more sources
Structural transitions upon guide RNA binding and their importance in Cas12g-mediated RNA cleavage. [PDF]
PLoS Genetics, 2023 Cas12g is an endonuclease belonging to the type V RNA-guided CRISPR-Cas family. It is known for its ability to cleave RNA substrates using a conserved endonuclease active site located in the RuvC domain. In this study, we determined the crystal structure
Mengxi Liu +8 more
doaj +3 more sources
The engineered single guide RNA structure as a biomarker for gene-editing reagent exposure [PDF]
Scientific Reports, 2023 CRISPR arrays and CRISPR-associated (Cas) proteins comprise a prevalent adaptive immune system in bacteria and archaea. These systems defend against exogenous parasitic mobile genetic elements.
Emmarie C. Ryan +2 more
doaj +2 more sources
PlmCas12e (CasX2) cleavage of CCR5: impact of guide RNA spacer length and PAM sequence on cleavage activity [PDF]
RNA Biology, 2023 Gene editing using CRISPR/Cas (clustered regularly interspaced palindromic repeats/CRISPR-associated) is under development as a therapeutic tool for the modification of genes in eukaryotic cells.
David A. Armstrong +5 more
doaj +2 more sources
Structure of yeast Argonaute with guide RNA [PDF]
Nature, 2012 The RNA-induced silencing complex, comprising Argonaute and guide RNA, mediates RNA interference. Here we report the 3.2 Å crystal structure of Kluyveromyces polysporus Argonaute (KpAGO) fortuitously complexed with guide RNA originating from small-RNA ...
Bartel, David +3 more
core +6 more sources
A Simplified Guide RNA Synthesis Protocol for SNAP- and Halo-Tag-Based RNA Editing Tools [PDF]
MoleculesSNAP-tag and Halo-tag have been employed to achieve targeted RNA editing by directing the deaminase domain of human ADAR to specific sites in the transcriptome.
Daniel Tobias Hofacker +4 more
doaj +2 more sources
Type I-E CRISPR-cas systems discriminate target from non-target DNA through base pairing-independent PAM recognition. [PDF]
PLoS Genetics, 2013 Discriminating self and non-self is a universal requirement of immune systems. Adaptive immune systems in prokaryotes are centered around repetitive loci called CRISPRs (clustered regularly interspaced short palindromic repeat), into which invader DNA ...
Edze R Westra +6 more
doaj +3 more sources
Guiding Bacteria with Small Molecules and RNA [PDF]
Journal of the American Chemical Society, 2007 Chemotactic bacteria navigate their chemical environment by coupling sophisticated information processing capabilities to molecular motors that propel the cells forward. The ability to reprogram bacteria to follow entirely new chemical signals would create powerful new opportunities in bioremediation, bionanotechnology, and synthetic biology.
Shana Topp, Justin P. Gallivan
openalex +3 more sources