RNA Interference by Single- and Double-stranded siRNA With a DNA Extension Containing a 3′ Nuclease-resistant Mini-hairpin Structure [PDF]
Molecular Therapy: Nucleic Acids, 2014 Selective gene silencing by RNA interference (RNAi) involves double-stranded small interfering RNA (ds siRNA) composed of single-stranded (ss) guide and passenger RNAs.
Simon J Allison, Jo Milner
doaj +11 more sources
Engineering CRISPR guide RNAs for programmable RNA sensors [PDF]
Biochemical Society Transactions, 2023 As the most valuable feature of the CRISPR system, the programmability based on Watson–Crick base pairing has been widely exploited in engineering RNA sensors. The base pairing in these systems offers a connection between the RNA of interest and the CRISPR effector, providing a highly specific mechanism for RNA detection both in vivo and in vitro.
Yang Liu, Wei Liu, Baojun Wang
openalex +4 more sources
Structural transitions upon guide RNA binding and their importance in Cas12g-mediated RNA cleavage. [PDF]
PLoS Genetics, 2023 Cas12g is an endonuclease belonging to the type V RNA-guided CRISPR-Cas family. It is known for its ability to cleave RNA substrates using a conserved endonuclease active site located in the RuvC domain. In this study, we determined the crystal structure
Mengxi Liu +8 more
doaj +3 more sources
Precise in vivo RNA base editing with a wobble-enhanced circular CLUSTER guide RNA. [PDF]
Nat BiotechnolRecruiting the endogenous editing enzyme adenosine deaminase acting on RNA (ADAR) with tailored guide RNAs for adenosine-to-inosine (A-to-I) RNA base editing is promising for safely manipulating genetic information at the RNA level.
Reautschnig P +16 more
europepmc +2 more sources
The engineered single guide RNA structure as a biomarker for gene-editing reagent exposure [PDF]
Scientific Reports, 2023 CRISPR arrays and CRISPR-associated (Cas) proteins comprise a prevalent adaptive immune system in bacteria and archaea. These systems defend against exogenous parasitic mobile genetic elements.
Emmarie C. Ryan +2 more
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Accommodation of Helical Imperfections in Rhodobacter sphaeroides Argonaute Ternary Complexes with Guide RNA and Target DNA [PDF]
Cell Reports, 2018 Summary: Prokaryotic Argonaute (Ago) proteins were recently shown to target foreign genetic elements, thus making them a perfect model for studies of interference mechanisms.
Yiwei Liu +6 more
doaj +4 more sources
DeepCRISPR: optimized CRISPR guide RNA design by deep learning
Genome Biology, 2018 A major challenge for effective application of CRISPR systems is to accurately predict the single guide RNA (sgRNA) on-target knockout efficacy and off-target profile, which would facilitate the optimized design of sgRNAs with high sensitivity and ...
Guohui Chuai +14 more
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A Simplified Guide RNA Synthesis Protocol for SNAP- and Halo-Tag-Based RNA Editing Tools [PDF]
MoleculesSNAP-tag and Halo-tag have been employed to achieve targeted RNA editing by directing the deaminase domain of human ADAR to specific sites in the transcriptome.
Daniel Tobias Hofacker +4 more
doaj +2 more sources
PLoS Genetics, 2020 Plant RNA viruses are used as delivery vectors for their high level of accumulation and efficient spread during virus multiplication and movement. Utilizing this concept, several viral-based guide RNA delivery platforms for CRISPR-Cas9 genome editing ...
Basudev Ghoshal +5 more
doaj +2 more sources
Multi-locus CRISPRi targeting with a single truncated guide RNA [PDF]
Nature CommunicationsA critical goal in functional genomics is evaluating which non-coding elements contribute to gene expression, cellular function, and disease. Functional characterization remains a challenge due to the abundance and complexity of candidate elements. Here,
Molly M. Moore +14 more
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