Exploring the dynamics of a single vesicle induced by Fe₃O₄ nanoparticles using micropipette manipulation. [PDF]
PLoS ONEMagnetite nanoparticles (MNPs, Fe3O4) have gained substantial interest for different biomedical and biochemical applications. Therefore, it is important to understand the mechanism of interaction between MNPs and cell membranes.
Nazia Ahmed +2 more
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The impact of sphingomyelin and cholesterol on ordered lipid domain formation in the bovine milk fat globule membrane using artificial giant unilamellar vesicles as a model [PDF]
JDS CommunicationsGiant unilamellar vesicle (GUV) bilayers were constructed from polar lipids and cholesterol by electroformation as a model system to investigate the formation of ordered lipid domains (OLD) within the milk fat globule membrane (MFGM).
Haotian Zheng +2 more
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Dimensions, stability, and deformability of DOPC-cholesterol giant unilamellar vesicles formed by droplet transfer [version 3; peer review: 2 approved, 2 approved with reservations] [PDF]
Open Research EuropeBackground Understanding cell membrane-like lipid bilayers is crucial for studying fundamental biological mechanisms. Giant Unilamellar Vesicles (GUVs) are key tools for this investigation and have applications in both synthetic biology and, more ...
Stefano Palagi +3 more
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Reconstitution of circadian clock in synthetic cells reveals principles of timekeeping [PDF]
Nature CommunicationsThe cyanobacterial circadian clock maintains remarkable precision and synchrony, even in cells with femtoliter volumes. Here, we reconstitute the KaiABC post-translational oscillator (PTO) in giant unilamellar vesicles (GUVs) to investigate underlying ...
Alexander Zhan Tu Li +2 more
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GUV long-term measurements of total ozone column and effective cloud transmittance at three Norwegian sites [PDF]
Atmospheric Chemistry and Physics, 2021 Measurements of total ozone column and effective cloud transmittance have been performed since 1995 at the three Norwegian sites Oslo/Kjeller, Andøya/Tromsø, and in Ny-Ålesund (Svalbard).
T. M. Svendby +8 more
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Functional reconstitution of plant plasma membrane H+-ATPase into giant unilamellar vesicles [PDF]
Scientific ReportsMembrane transporters are essential for numerous biological processes by controlling the movement of ions and molecules across cell membranes. However, dissecting their molecular dynamics in complex cellular environments presents significant challenges ...
Huriye D. Uzun +3 more
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Whole-GUV patch-clamping [PDF]
Proceedings of the National Academy of Sciences, 2016 Significance Although membrane composition and tension modulate the activity of ion channels and transporters, this protein–membrane coupling has been challenging to study due to the difficulty of controlling membrane properties in cells and technical limitations of existing in vitro systems.
Garten, Matthias +5 more
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Scientific Reports, 2023 Membrane fusion is an essential step for the entry of enveloped viruses, such as human immunodeficiency virus and influenza virus, into the host cell, often triggered by the binding of membrane proteins on the viral envelope to host cell membrane ...
Hiroto Furukawa +3 more
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Frontiers in Molecular Biosciences, 2020 Depending on the physical and biochemical properties of actin-binding proteins, actin networks form different types of membrane protrusions at the cell periphery.
Yashar Bashirzadeh +5 more
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Methods to mechanically perturb and characterize GUV-based minimal cell models
Computational and Structural Biotechnology Journal, 2023 Cells shield organelles and the cytosol via an active boundary predominantly made of phospholipids and membrane proteins, yet allowing communication between the intracellular and extracellular environment.
Nadab H. Wubshet, Allen P. Liu
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