Results 81 to 90 of about 16,350 (151)
CNS Neuroscience & TherapeuticsAbstractN6‐methyladenosine (m6A) methylation is a vital epigenetic mechanism associated with drug addiction. However, the relationship between m6A modification and oxycodone rewarding is less well explored. Based on an open field test, the present study evaluated oxycodone rewarding using chromatin immunoprecipitation PCR, immunofluorescence, and RNA ...
Tian‐Cong Liu +6 more
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Additional file 4: Figure S4. An artificial H3K27me3-H3K4me1 transition regulates the ESCs differentiation. A Western blotting analysis of H3K27me3 modification in WT and Suz12−/− OG2 mESCs. H3 as the loading control; heatmap showing H3K27me3 patterns of promoter CGIs and their shores in WT, Eed−/− (replicate) and SUZ12−/− OG2 mESCs.
Yu, Yue +5 more
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Additional file 5: Figure S5. LSD1 interacts with core members of PRC2 and plays a significant role in the artificial H3K27me3-H3K4me1 transition in mESCs. A Heatmaps and average line plots showing histone modifications patterns of promoter CGIs and their shores in WT, Mll2-shRNA and Mll3-shRNA mESCs. Each line represents a single CpG island.
Yu, Yue +5 more
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Additional file 6: Figure S6. Lsd1 knockout induces similar effects as Eed or Suz12 knockout. A Western blot analysis of LSD1 protein in each indicated cell lines (OG2, OG2-Lsd1−/−). ACTIN as the loading control. B Heatmaps and average line plots showing H3K4me1, H3K4me3 and H3K27me3 patterns at promoter CGIs and their shores of two different groups in
Yu, Yue +5 more
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Additional file 1: Figure S1. H3K4me1 in combination with H3K4me3 is able to partition promoters and predict bivalent promoters. A Heatmaps showing histone modifications patterns of promoter CGIs and their shores based on the distribution patterns of traditional bivalent marks (H3K4me3 and H3K27me3) (left) or non-traditional bivalent marks (H3K4me1 and
Yu, Yue +5 more
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Additional file 7: Figure S7. Uncropped scans of western blots. A Uncropped scans of western blots for Fig. 4A and Fig. S4A. Histone modification H3K27me3 level was analyzed by western-blot using the specific antibody on the whole cell lysates from indicated cell lines (OG2, OG2-Eed−/− and OG2-Suz12−/−). H3 as the loading control. Red box indicates the
Yu, Yue +5 more
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Corrigendum: H3K4me1 marks DNA regions hypomethylated during aging in human stem and differentiated cells [PDF]
Genome Research, 2019 Agustín F, Fernández +29 more
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, 2020 From simple double-stranded molecule DNA is packed in the nucleus via different intermediate structures with help of various proteins organizing a complex structure referred to as chromatin. A subunit of chromatin is a nucleosome that reminiscent a reel in which the DNA region is winded around a core of proteins which are called histones.
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MBD8 is required for LDL2-mediated transcriptional repression downstream of H3K9me2 in Arabidopsis. [PDF]
Nucleic Acids ResMori S +6 more
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Protocol for genomic mapping of chromatin targets using high-throughput CUT&RUN. [PDF]
STAR ProtocKotapalli V, Iakova P, Lu Y, Jain AK.
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