Results 81 to 90 of about 6,706 (158)

Assimilatory nitrate reductase from the haloarchaeon Haloferax mediterranei: purification and characterisation [PDF]

FEMS Microbiology Letters, 2001
Haloferax mediterranei can use nitrate as sole nitrogen source during aerobic growth. We report here the purification and biochemical characterisation of the assimilatory nitrate reductase (EC 1.6.6.2) from H. mediterranei. The enzyme, as isolated, was composed of two subunits (105+/-1.3 kDa and 50+/-1.3 kDa) and behaved as a dimer during gel ...
R M, Martínez-Espinosa, F C, Marhuenda-Egea, M J, Bonete   +2 more
openaire   +2 more sources

FISH, a new tool for in situ preservation of RNA in tissues of deep‐sea mobile fauna

Limnology and Oceanography: Methods, Volume 23, Issue 4, Page 273-292, April 2025.
ABSTRACT Accessing the metabolic functioning of deep‐sea animals in situ remains a technological challenge as the recovery time of samples is incompatible with the short lifespan of such molecules as mRNAs. Tools able to preserve RNA in situ exist, but they are incompatible with the study of mobile fauna.
Valérie Cueff‐Gauchard, Johanne Aubé, Jean‐Romain Lagadec, Laurent Bignon, Jean‐Pierre Lafontaine, Iván Hernández‐Ávila, Nathalie Marsaud, Bruce Shillito, Louis Amand, Erwan G. Roussel, Marie‐Anne Cambon   +10 more
wiley   +1 more source

The effect of ammonium on assimilatory nitrate reduction in the haloarchaeon Haloferax mediterranei

Extremophiles, 2007
Physiology, regulation and biochemical aspects of the nitrogen assimilation are well known in Prokarya or Eukarya but they are poorly described in Archaea domain. The haloarchaeon Haloferax mediterranei can use different nitrogen inorganic sources (NO (3) (-) , NO (2) (-) or NH (4) (+) ) for growth.
Rosa María, Martínez-Espinosa, Belén, Lledó, Frutos C, Marhuenda-Egea, María José, Bonete   +3 more
openaire   +3 more sources

A Spotlight on Archaea in Humans, Livestock and Poultry: A Review

Veterinary Medicine and Science, Volume 11, Issue 2, March 2025.
The microbiota includes prokaryotes (archaea and bacteria) and eukaryotes. Archaea are single‐celled prokaryotes and essential part of gut microbiome. Researches on archaea in ruminants and humans are more than mono‐gastric. The low abundance of archaea in the gut depends on the method used (metagenomics or meta‐transcriptomic) and age of people or ...
Ahmad Salahi, Wafaa A. Abd El‐Ghany
wiley   +1 more source

Cu-NirK from Haloferax mediterranei as an example of metalloprotein maturation and exportation via Tat system

, 2013
The green Cu-NirK from Haloferax mediterranei (Cu-NirK) has been expressed, refolded and retrieved as a trimeric enzyme using an expression method developed for halophilic Archaea.
Martínez-Espinosa, Rosa María, Zafrilla, Basilio, Esclapez, Julia, Bonete, María-José   +3 more
core   +1 more source

Glucose dehydrogenase from the halophilic Archaeon Haloferax mediterranei: Enzyme purification, characterisation and N-terminal sequence [PDF]

, 1996
An NAD(P)-glucose dehydrogenase from the extremely halophilic Archaeon, Haloferax mediterranei, has been purified to electrophoretic homogeneity. The purified enzyme has been characterised with respect to its cofactor specificity, subunit composition and
Llorca, Francisco I., María-José Bonete, Carmen Pire, Pire, Carmen, Mónica L. Camacho, Bonete, María-José, Francisco I. Llorca, Camacho, Mónica L.   +7 more
core   +1 more source

Role of CRISPR‐Cas systems in periodontal disease pathogenesis and potential for periodontal therapy: A review

Molecular Oral Microbiology, Volume 40, Issue 1, Page 1-16, February 2025.
Abstract Clustered regularly interspaced short palindromic repeats (CRISPRs) are DNA sequences capable of editing a host genome sequence. CRISPR and its specific CRISPR‐associated (Cas) protein complexes have been adapted for various applications. These include activating or inhibiting specific genetic sequences or acting as molecular scissors to cut ...
Aditi Chopra, Geeta Bhuvanagiri, Kshitija Natu, Avneesh Chopra   +3 more
wiley   +1 more source

Application of CRISPR Technology in Plant Improvement: An Update Review

Advances in Agriculture, Volume 2025, Issue 1, 2025.
Clustered regularly interspaced short palindromic repeats/CRISPR‐associated protein 9 (CRISPR/Cas9) stands as a major technique in genetic engineering, offering innumerable applications for enhancing agricultural and horticultural crops. It contributes significantly to improving quality traits, managing biotic and abiotic stresses, and extending shelf ...
Himanshu Saini, Avantika Devrani, Gideon Synrem, Priyanka, Khaled F. M. Salem   +4 more
wiley   +1 more source

Analysis of acidic surface of Haloferax mediterranei glucose dehydrogenase by site-directed mutagenesis [PDF]

, 2007
Generally, halophilic enzymes present a characteristic amino acid composition, showing an increase in the content of acidic residues and a decrease in the content of basic residues, particularly lysines.
Bonete, M.J., Ferrer, J., M.J. Bonete, R.M. Martínez-Espinosa, Pire, C., V. Bautista, J. Ferrer, Esclapez, J., C. Pire, Martínez-Espinosa, R.M., Bautista, V., J. Esclapez   +11 more
core   +1 more source

A comprehensive review on types and properties of biopolymers as sustainable bio‐based alternatives for packaging

Food Biomacromolecules, Volume 1, Issue 2, Page 58-87, December 2024.
Abstract Biopolymers, derived from renewable resources such as plants, animals, and microorganisms, are emerging as sustainable alternatives to traditional petroleum‐based polymers. The review examines the key characteristics of biopolymers, including their biodegradability, biocompatibility, and potential for carbon neutrality.
Ditimoni Dutta, Nandan Sit
wiley   +1 more source