Results 21 to 30 of about 19,297 (229)

Characterization and Comparison of Ochratoxin A-Ovalbumin (OTA-OVA) Conjugation by Three Methods

Journal of Integrative Agriculture, 2014
In order to generate an antibody against a small hapten molecule, the hapten is cross-linked with carrier protein to make it immunogenic. In this study, the hapten (ochratoxin A, OTA) was coupled to ovalbumin (OVA) by an active ester reaction. To develop
Xi-chun WANG, Ming BAO, Jin-jie WU, Ying LUO, Liang-you MA, Ying WANG, Ai-hua ZHANG, Cheng-hua HE, Hai-bin ZHANG   +8 more
doaj   +1 more source

Design, Synthesis, and In Vivo Evaluation of C1-Linked 4,5-Epoxymorphinan Haptens for Heroin Vaccines

Molecules, 2022
In our continuing effort to develop effective anti-heroin vaccines as potential medications for the treatment of opioid use disorder, herein we present the design and synthesis of the haptens: 1-AmidoMorHap (1), 1-AmidoMorHap epimer (2), 1 Amido ...
Agnieszka Sulima, Fuying Li, Jeffrey Brian Morgan, Phong Truong, Joshua F. G. Antoline, Therese Oertel, Rodell C. Barrientos, Oscar B. Torres, Zoltan Beck, Gregory H. Imler, Jeffrey R. Deschamps, Gary R. Matyas, Arthur E. Jacobson, Kenner C. Rice   +13 more
doaj   +1 more source

A chemically contiguous hapten approach for a heroin–fentanyl vaccine

Beilstein Journal of Organic Chemistry, 2019
Background: Increased death due to the opioid epidemic in the United States has necessitated the development of new strategies to treat addiction. Monoclonal antibodies and antidrug vaccines provide a tool that both aids addiction management and reduces ...
Yoshihiro Natori, Candy S. Hwang, Lucy Lin, Lauren C. Smith, Bin Zhou, Kim D. Janda   +5 more
doaj   +1 more source

Preparation of Monoclonal Antibodies Specifically Reacting with the Trichothecene Mycotoxins Nivalenol and 15-Acetylnivalenol via the Introduction of a Linker Molecule into Its C-15 Position

Toxins, 2022
Nivalenol (NIV) is a trichothecene mycotoxin that is more toxic than deoxynivalenol. It accumulates in grains due to infection with Fusarium species, which are the causative agents of scab or Fusarium head blight.
Kyoko Noda, Yuki Hirakawa, Tomomi Nishino, Ritsuto Sekizuka, Marin Kishimoto, Tomohiro Furukawa, Sakiko Sawane, Ayu Matsunaga, Naoki Kobayashi, Kazutoshi Sugita, Kenji Oonaka, Hiroko Kawakami, Yuji Otsuka, Tetsuya Yamamoto, Toshihiro Yamamoto, Taku Yoshiya, Maiko Watanabe, Machiko Saka, Keiko Momma, Masayo Kushiro, Shiro Miyake   +20 more
doaj   +1 more source

Synthesis of Polyclonal Antibodies against Aflatoxin B1

Makara Journal of Science, 2015
Polyclonal antibodies of aflatoxin B1 were successfully produced from New Zealand White female rabbits after immunization by the hapten of aflatoxin B1-carboxymethyl hydroxylamine hemihydrochloride (AFB1-CMO) conjugated with bovine serum albumin (BSA) as
Wiyogo Prio Wicaksono, Deni Samsudin Permana, Romsyah Maryam, Yasuaki Einaga   +3 more
doaj   +1 more source

Hapten-Induced Contact Hypersensitivity, Autoimmune Reactions, and Tumor Regression: Plausibility of Mediating Antitumor Immunity

Journal of Immunology Research, 2014
Haptens are small molecule irritants that bind to proteins and elicit an immune response. Haptens have been commonly used to study allergic contact dermatitis (ACD) using animal contact hypersensitivity (CHS) models.
Dan A. Erkes, Senthamil R. Selvan
doaj   +1 more source

Chemical- and Drug-Induced Allergic, Inflammatory, and Autoimmune Diseases Via Haptenation

Biology, 2023
Haptens are small molecules that only elicit an immune response when bound to proteins. Haptens initially bind to self-proteins and activate innate immune responses by complex mechanisms via inflammatory cytokines and damage-associated molecular patterns
Eri Sakamoto, Yasuhiro Katahira, Izuru Mizoguchi, Aruma Watanabe, Yuma Furusaka, Ami Sekine, Miu Yamagishi, Jukito Sonoda, Satomi Miyakawa, Shinya Inoue, Hideaki Hasegawa, Kazuyuki Yo, Fumiya Yamaji, Akemi Toyoda, Takayuki Yoshimoto   +14 more
doaj   +1 more source

Role of hydrophobic interaction in hapten-antibody binding

, 1977
The precipitation reaction of bovine serum albumin coupled with p-azophenylleucine with homologous antibody was inhibited by several structurally related haptens. The isobutyl group substituent on alpha-carbon atom of the leucine residue contributed more
Oda, Takuzo, Tsutsui, Ken, Hayashi, Hideo, Hatase, Osamu, Koide, Noriko, Tomoda, Jun   +5 more
core   +1 more source

Development of Methamphetamine Conjugated Vaccine through Hapten Design: In Vitro and In Vivo Characterization

Vaccines, 2023
Background: Methamphetamine (METH) substance-use disorder is an ever-growing global health issue with no effective treatment. Anti-METH vaccines are under investigation as an alternative to existing psychological interventions.
Md Kamal Hossain, Majid Davidson, Jack Feehan, George Deraos, Kulmira Nurgali, John Matsoukas, Vasso Apostolopoulos   +6 more
doaj   +1 more source

Hapten-Specific Single-Cell Selection of Hybridoma Clones by Fluorescence-Activated Cell Sorting for the Generation of Monoclonal Antibodies

, 2017
The conventional hybridoma screening and subcloning process is generally considered to be one of the most critical steps in hapten-specific antibody production.
Schenk, J. A., Kuhne, Maren, Hoffmann, Katrin, Dippong, Martin, Lenz, C., Peter Carl, Maren Kuhne, Rudolf J. Schneider (3699694), Schneider, Rudolf, Schwaar, Timm, Lenz, Christine, Katrin Hoffmann, Martin Dippong (1857256), Timm Schwaar, Jörg A. Schenk, Martin Dippong, Schenk, Jörg A., Carl, Peter, Timm Schwaar (1514116), Katrin Hoffmann (17460), Jörg A. Schenk (3852733), Schneider, Rudolf J., Maren Kuhne (58602), Rudolf J. Schneider, Christine Lenz, Peter Carl (3852736), Christine Lenz (244370)   +26 more
core   +1 more source