Results 21 to 30 of about 15,180 (184)
Charakterisierung von Immunseren durch Bindung von monovalentem Penicilloylhapten
CHIMIA, 1976 The preparation of tritium-labeled Nβ-benzylpenicilloylalanine and its use as a monovalent hapten in binding studies is described. The binding of 10-8M hapten to antisera of various species is readily performed but insufficient test sensitivity hampers ...
U. Otz, C.H. Schneider, A.L. de Weck
doaj +1 more source
Modulating Linker Composition of Haptens Resulted in Improved Immunoassay for Histamine
Biomolecules, 2019 Histamine (HA) is an important food contaminant generated during food fermentation or spoilage. However, an immunoassay for direct (derivatization free) determination of HA has rarely been reported due to its small size to induce the desired antibodies ...
Lin Luo +9 more
doaj +1 more source
Experimental Biology and Medicine, 1926 Abstract In attempting to purify the specific part of the heterogenetic haptene, present in alcoholic extracts of horse kidneys, fractions were obtained which are distinguished from the common lipoids by their solubility in water. The material of one fraction described recently3 is practically insoluble in alcohol, chloroform and ether ...
K Landsteiner, P A Levene
openaire +1 more source
Synthesis and identification of the antigens for carbofuran
浙江大学学报. 农业与生命科学版, 2002 This study reported that the first compound, 2, 3-dihydro-2, 2-dimethyl-7-benfuranyl chloroformate (DDBC) was synthesized after using 2,3-dihydro-2,2-dimrthyl-7-benzofuranol reacted with CoCl2.
ZHU Guo-nian +2 more
doaj +1 more source
PLoS ONE, 2019 Hapten contact hypersensitivity (CHS) elicits a well-documented inflammation response that can be used to illustrate training of immune cells through hapten-specific CHS memory.
E J Putz +10 more
doaj +1 more source
Frontiers in Nutrition, 2022 The common carbamate insecticide aldicarb is considered one of the most acutely toxic pesticides. Herein, rational design was used to synthesize two haptens with spacers of different carbon chain lengths. The haptens were then used to immunize mice.
Hong Shen +20 more
doaj +1 more source
Predictable Peptide Conjugation Ratios by Activation of Proteins with Succinimidyl Iodoacetate (SIA)
Methods and Protocols, 2017 The small heterobifunctional linker succinimidyl iodoacetate (SIA) was examined for the preparation of peptide–protein bioconjugates with predicable conjugation ratios.
Ioana M. Abbas +3 more
doaj +1 more source
Simple Method for Preparation of Fluor/Hapten-Labeled dUTP
BioTechniques, 2000 Many projects, such as multiplex-fluorescence in situ hybridization (M-FISH) karyotyping, require the use of relatively large amounts of multiple fluor- or hapten-labeled nucleotides for the preparation of DNA probes.
Manjunath Nimmakayalu +3 more
doaj +1 more source
Characterization and Comparison of Ochratoxin A-Ovalbumin (OTA-OVA) Conjugation by Three Methods
Journal of Integrative Agriculture, 2014 In order to generate an antibody against a small hapten molecule, the hapten is cross-linked with carrier protein to make it immunogenic. In this study, the hapten (ochratoxin A, OTA) was coupled to ovalbumin (OVA) by an active ester reaction. To develop
Xi-chun WANG +8 more
doaj +1 more source
Molecules, 2022 In our continuing effort to develop effective anti-heroin vaccines as potential medications for the treatment of opioid use disorder, herein we present the design and synthesis of the haptens: 1-AmidoMorHap (1), 1-AmidoMorHap epimer (2), 1 Amido ...
Agnieszka Sulima +13 more
doaj +1 more source