Results 111 to 120 of about 150,776 (304)

Combining Fret and Super‐Resolution Microscopy Reveals Kinase Activation and Mitochondrial Activity at the Nanoscale

Advanced Science, EarlyView.
BioSenSRRF is an open‐source workflow that combines conventional FRET biosensors with SRRF reconstruction to generate sub‐diffraction FRET index maps on standard microscopes. The pipeline integrates automated image registration, SRRF reconstruction, quantitative FRET index calculation, and random line‐based hotspot analysis to uncover AURKA‐dependent ...
Nicolas Y. Jolivet, Pierre‐Jean Desmaison, Xavier Pinson, Arthur Masson, Olivier Delalande, Giulia Bertolin   +5 more
wiley   +1 more source

GAPDH suppresses adenovirus-induced oxidative stress and enables a superfast production of recombinant adenovirus

Genes and Diseases
Recombinant adenovirus (rAdV) is a commonly used vector system for gene transfer. Efficient initial packaging and subsequent production of rAdV remains time-consuming and labor-intensive, possibly attributable to rAdV infection-associated oxidative ...
Guozhi Zhao, Piao Zhao, Yonghui Wang, Hui Zhang, Yi Zhu, Jiamin Zhong, Wulin You, Guowei Shen, Changqi Luo, Ou Mei, Xingye Wu, Jingjing Li, Yi Shu, Hongwei Wang, William Wagstaff, Hue H. Luu, Yang Bi, Lewis L. Shi, Russell R. Reid, Tong-Chuan He, Li Jiang, Wei Tang, Jiaming Fan, Ziwei Tang   +23 more
doaj   +1 more source

Use of Short-Lived Green Fluorescent Protein for the Detection of Proteasome Inhibition

BioTechniques, 2001
Human embryonic kidney (HEK293) cells were stably transduced with a retroviral vector containing an expression cassette for a short-lived green fluorescent protein (d2EGFP) and the neomycin resistance gene (Neor).
C. Andreatta, P. Nahreini, A.R. Hovland, B. Kumar, J. Edwards-Prasad, K.N. Prasad   +5 more
doaj   +1 more source

RCAN1 is neddylated in HEK293 cells.

, 2012
(A) HEK293 cells were transfected for 24 h with HA-tagged RCAN1 and/or T7-tagged NEDD8 plasmids. Immunoprecipitation (IP) was performed with HA antibodies, and the immunocomplexes were analyzed by western blotting with T7 antibodies.
Eunju Im (122867), Kwang Chul Chung (122870), Hee Sun Hwang (122863), Boram Min (122864), Eun Hye Noh (122859), Hee Sook Hwang (122861)   +5 more
core   +1 more source

MG53 Coordinates Macrophage Polarization and Neuroimmune Coupling to Promote Corneal Nerve Regeneration via the MPEG1–MVP–STAT6 Axis

Advanced Science, EarlyView.
Corneal nerve regeneration is critical to corneal wound healing processes. The current study reveals a novel role of MG53 in promoting corneal nerve regeneration after alkali induced injury. Mechanistically, MG53 enters macrophages via its receptor, MPEG1, promotes MVP K63 ubiquitination, and triggers STAT6 induced repair‐related genes expression ...
Peng Chen, Zhentao Zhang, Lilian Sakai, Yanping Xu, Luxi Zhang, Han Ye, William Sollenberger, Takahide Ikeda, Zhiyu Yan, Keerthika Sathish, Usman Alizai, Haitao Wen, Timothy M. Pawlik, Heather L. Chandler, Hua Zhu   +14 more
wiley   +1 more source

Construction of HEK293 and C2C12 cell models transfected by LMNA mutant and related intracellular sublocalization of lamin A/C [PDF]

Jichu yixue yu linchuang, 2020
Objective To construct an expression vector of fusion protein from wild type LMNA and mutant and the lentivirus vector of LMNA mutant, to study the expression and sublocalization of lamin A/C and the change of nucleus in HEK293 and C2C12 cells.
TAN Dan-dan, CHAI Jing-yan, LIU Jian-yun, NIE Hong-bing, XIONG Hui, WU Xiang-bin
doaj  

Cytoprotective effect of glutaraldehyde erythropoietin on HEK293 kidney cells after silver nanoparticle exposure

, 2016
Kanidta Sooklert,1,2 Supreecha Chattong,3 Krissanapong Manotham,3 Chawikan Boonwong,1 I-yanut Klaharn,1 Depicha Jindatip,4 Amornpun Sereemaspun1,4 1Nanobiomedicine Laboratory, Department of Anatomy, Faculty of Medicine, 2Inter-Department Program of ...
Klaharn I, Chattong S, Manotham K, Jindatip D, Sereemaspun A, Sooklert K, Boonwong C   +6 more
core  

Function of MYCT1-TV and MYCT1 in Hep2 and HEK293 cells.

, 2013
The proliferation of Hep2 and HEK293 cells. B. The apoptosis of Hep2 and HEK293 cells. C. The invasive ability of Hep2 and HEK293 cells. Blank, control cells before transfection; GFP, control cells after transfection with GFP only; MYCT1-TV, cells ...
Wei-Neng Fu (342688), Zhen-Ming Xu (342685), Ming Zhong (151097), Shuang Fu (342684), Kai-Lai Sun (342687), Guang-Bin Qiu (342686), Yan Guo (83742), Hong Chen (108084)   +7 more
core   +1 more source

Orthogonal Ionic Liquid‐Based Extraction Strategy Enables Amyloid‐Specific Profiling of Aggregate Proteome

Advanced Science, EarlyView.
An orthogonal ionic‐liquid extraction (Orth‐iEA) enables selective isolation of amyloid fibrils. TMGBF4 disrupts hydrogen‐bonded β‐sheet networks to solubilize amyloid aggregates, whereas C12ImCl interacts with hydrophobic regions of non‐amyloid proteins.
Shiying Zheng, Ye Liu, Bowen Zhong, Huiying Chu, Zhou Gong, Baofeng Zhao, Mengchun Cheng, Zhen Liang, Yukui Zhang, Qun Zhao, Lihua Zhang   +10 more
wiley   +1 more source

Neurokinin 1 receptor mediates membrane blebbing and sheer stress-induced microparticle formation in HEK293 cells.

PLoS ONE, 2012
Cell-derived microparticles participate in intercellular communication similar to the classical messenger systems of small and macro-molecules that bind to specialized membrane receptors. Microparticles have been implicated in the regulation of a variety
Panpan Chen, Steven D Douglas, John Meshki, Florin Tuluc   +3 more
doaj   +1 more source