Results 121 to 130 of about 132,829 (164)

Molecular characterization of HEK293 cells as emerging versatile cell factories

Current Opinion in Biotechnology, 2021
HEK293 cell lines are used for the production of recombinant proteins, virus-like particles and viral vectors. Recent work has generated molecular (systems level) characterisation of HEK293 variants that has enabled re-engineering of the cells towards enhanced use for manufacture-scale production of recombinant biopharmaceuticals (assessment of 'safe ...
Pulix, Michela, Lukashchuk, Vera, Smith, Daniel C, Dickson, Alan J   +3 more
openaire   +5 more sources

Functional Reconstruction of NLRs in HEK293 Cells

2016
Inflammasomes are molecular complexes that initiate innate immune response. They are mainly expressed by immune cells; however, molecular manipulations in these cells remain very difficult. Here, we describe a simple protocol to overexpress and activate functional NRLP3 inflammasomes in HEK293 cells.
Compan, Vincent, Lopez-Castejon, Gloria
openaire   +4 more sources

Upregulation of PHLDA2 in Dicer knockdown HEK293 cells

Biochimica et Biophysica Acta (BBA) - General Subjects, 2007
It has been reported that RNAi-dependent chromatin silencing in vertebrates is not restricted to the centromeres. To address whether RNAi machinery could regulate the chromatin structure of imprinted genes, we knocked down Dicer in HEK293 cells and found that the expression of PHLDA2, one of the several genes in the imprinted gene domain of 11p15.5 ...
Kai-Fu, Tang, Yan, Wang, Pengfei, Wang, Min, Chen, Yao, Chen, Huai-Dong, Hu, Peng, Hu, Bo, Wang, Wenjie, Yang, Hong, Ren   +9 more
openaire   +2 more sources

Transient Gene Expression in Suspension HEK293-EBNA1 Cells

2018
Transient gene expression in human embryo kidney 293 (HEK293) cells is an established approach for the rapid production of large amounts of recombinant proteins (r-proteins). Milligram to gram quantities of r-proteins can be typically obtained within less than 10 days following transfection. In this chapter, we describe a simple and robust transfection
L’Abbé, Denis, Bisson, Louis, Gervais, Christian, Grazzini, Eric, Durocher, Yves   +4 more
openaire   +2 more sources

PIASy interacts with p73α and regulates cell cycle in HEK293 cells

Cellular Immunology, 2010
p73, a recently described member of the p53 family of transcription factors, undergoes a number of posttranslational modifications, inducing cell cycle arrest and apoptosis. In the present study, we demonstrate that PIASy, a member of the PIAS SUMO-ligase family, interacts with p73alpha, and that PIASy-mediated sumoylation promotes proteasomal ...
Chao, Zhang, Xia, Yuan, Ling, Yue, Jin, Fu, Lan, Luo, Zhimin, Yin   +5 more
openaire   +2 more sources

Low-level arsenite induced gene expression in HEK293 cells

Toxicology, 2003
Chronic, low-level exposure to arsenic frequently results in skin, lung, bladder, and kidney cancer. Since arsenic is primarily excreted via the kidney, this study focused on this target tissue. Gene array was used as a sensitive low-level monitor of the impact of arsenic on this target tissue.
Xing Hui, Zheng, George S, Watts, Skip, Vaught, A Jay, Gandolfi   +3 more
openaire   +2 more sources

Interferon inhibitors increase rAAV production in HEK293 cells

Journal of Biotechnology
Recombinant adeno-associated viruses (rAAVs) comprise a promising viral vector for therapeutic gene delivery to treat disease. However, the current manufacturing capability of rAAVs must be improved to meet commercial demand. Previously published omics studies indicate that rAAV production through transient transfection triggers antiviral responses and
Yongdan, Wang, Qiang, Fu, Sha, Sha, Seongkyu, Yoon   +3 more
openaire   +2 more sources

Transfected synphilin-1 forms cytoplasmic inclusions in HEK293 cells

Molecular Brain Research, 2001
The discovery of mutations in the gene for alpha-synuclein in familial Parkinson's disease (PD) has led to an increased interest in this pre-synaptic protein. Synphilin-1, a potential synuclein-binding protein, was cloned using yeast two-hybrid assays.
C, O'Farrell, D D, Murphy, L, Petrucelli, A B, Singleton, J, Hussey, M, Farrer, J, Hardy, D W, Dickson, M R, Cookson   +8 more
openaire   +2 more sources

Mitofusin-2 protects against cold stress-induced cell injury in HEK293 cells

Biochemical and Biophysical Research Communications, 2010
Mitochondrial impairment is hypothesized to contribute to cell injury during cold stress. Mitochondria fission and fusion are closely related in the function of the mitochondria, but the precise mechanisms whereby these processes regulate cell injury during cold stress remain to be determined. HEK293 cells were cultured in a cold environment (4.0+/-0.1
Wenbin, Zhang, Yaomin, Chen, Qun, Yang, Honglei, Che, Xiangjun, Chen, Ting, Yao, Fang, Zhao, Mingchao, Liu, Tao, Ke, Jingyuan, Chen, Wenjing, Luo   +10 more
openaire   +2 more sources