Results 11 to 20 of about 4,432 (222)

High-complexity extracellular barcoding using a viral hemagglutinin [PDF]

Proceedings of the National Academy of Sciences, 2020
While single-cell sequencing technologies have revealed tissue heterogeneity, resolving mixed cellular libraries into cellular clones is essential for many pooled screens and clonal lineage tracing. Fluorescent proteins are limited in number, while DNA barcodes can only be read after cell lysis.
Mullokandov, Gavriel, Vijayakumar, Gayathri, Leon, Paul, Henry, Carole, Wilson, Patrick C., Krammer, Florian, Palese, Peter, Brown, Brian D.   +7 more
openaire   +6 more sources

Characterization of the sialic acid binding activity of influenza A viruses using soluble variants of the H7 and H9 hemagglutinins. [PDF]

PLoS ONE, 2014
Binding of influenza viruses to target cells is mediated by the viral surface protein hemagglutinin. To determine the presence of binding sites for influenza A viruses on cells and tissues, soluble hemagglutinins of the H7 and H9 subtype were generated ...
Anne-Kathrin Sauer, Chi-Hui Liang, Jürgen Stech, Ben Peeters, Pascale Quéré, Christel Schwegmann-Wessels, Chung-Yi Wu, Chi-Huey Wong, Georg Herrler   +8 more
doaj   +3 more sources

Recognition of viral hemagglutinins by NKp44 but not by NKp30 [PDF]

European Journal of Immunology, 2001
Natural killer (NK) cells destroy virus-infected and tumor cells without prior antigen stimulation. The NK cell cytotoxicity is regulated in large part by the expression of NK cell receptors that are able to bind major histocompatibility complex (MHC) class I glycoproteins.
Arnon, T, Lev, M, Katz, G, Chernobrov, Y, Porgador, A, Mandelboim, O   +5 more
openaire   +4 more sources

Applying valency-based immuno-selection to generate broadly cross-reactive antibodies against influenza hemagglutinins [PDF]

Nature Communications
Conserved epitopes shared between virus subtypes are often subdominant, making it difficult to induce broadly reactive antibodies by immunization. Here, we generate a plasmid DNA mix vaccine that encodes protein heterodimers with sixteen different ...
Daniëla Maria Hinke, Ane Marie Anderson, Kirankumar Katta, Marlene Fyrstenberg Laursen, Demo Yemane Tesfaye, Ina Charlotta Werninghaus, Davide Angeletti, Gunnveig Grødeland, Bjarne Bogen, Ranveig Braathen   +9 more
doaj   +2 more sources

Immunogenicity of chimeric hemagglutinins delivered by an orf virus vector platform against swine influenza virus [PDF]

Frontiers in Immunology
Orf virus (ORFV) is a large DNA virus that can harbor and efficiently deliver viral antigens in swine. Here we used ORFV as a vector platform to deliver chimeric hemagglutinins (HA) of Influenza A virus of swine (IAV-S). Vaccine development against IAV-S
Gabriela Mansano do Nascimento, Pablo Sebastian Britto de Oliveira, Pablo Sebastian Britto de Oliveira, Salman Latif Butt, Diego G. Diel   +4 more
doaj   +3 more sources

A simple procedure for purification of viral hemagglutinin [PDF]

Virology, 1962
Peer Reviewed ; http://deepblue.lib.umich.edu/bitstream/2027.42/32311/1/0000379 ...
Mizutani, H., Beals, T., Hennessy, A. V., Davenport, Fred M.   +3 more
openaire   +3 more sources

Data for Polymer-stabilized sialylated nanoparticles : synthesis, optimization, and differential binding to influenza hemagglutinins [PDF]

, 2020
During influenza infection, hemagglutinins (HAs) on the viral surface bind to sialic acids on the host cell's surface. While all HAs bind sialic acids, human influenza targets terminal α2,6 sialic acids and avian influenza targets α2,3 sialic acids.
Richards, Sarah-Jane, Gibson, Matthew I., Baker, Alexander, Walker, Marc   +3 more
core   +4 more sources

Formation of influenza virus particles lacking hemagglutinin on the viral envelope [PDF]

Journal of Virology, 1986
We investigated the intracellular block in the transport of hemagglutinin (HA) and the role of HA in virus particle formation by using temperature-sensitive (ts) mutants (ts134 and ts61S) of influenza virus A/WSN/33. We found that at the nonpermissive temperature (39.5 degrees C), the exit of ts HA from the rough endoplasmic reticulum to the Golgi ...
Pattnaik, Asit K., Brown, Donald J., Nayak, Debi P.   +2 more
openaire   +2 more sources

Adjuvant-free immunization with hemagglutinin-Fc fusion proteins as an approach to influenza vaccines [PDF]

, 2011
The hemagglutinins (HAs) of human H1 and H3 influenza viruses and avian H5 influenza virus were produced as recombinant fusion proteins with the human immunoglobulin Fc domain.
Temperton, Nigel J., Ian M. Jones, Christopher R. Bailey, Eleonora Molesti, Ren, Junyuan, Jones, Ian M., Temperton, Nigel J, Silvia Loureiro, Pongsathon Phapugrangkul, Junyuan Ren, Wendy S. Barclay, Camilo A. Colaco, Molesti, Eleonora, Barclay, Wendy S., Shelton, Holly, Colaco, Camilo A, Phapugrangkul, Pongsathon, Nigel J. Temperton, Loureiro, Silvia, Barclay, Wendy S, Bailey, Christopher R, Holly Shelton, Jones, Ian, Colaco, Camilo A., Bailey, Christopher R.   +24 more
core   +1 more source

Structural Basis for Influence of Viral Glycans on Ligand Binding by Influenza Hemagglutinin [PDF]

Biophysical Journal, 2008
Binding of cell surface glycans by influenza hemagglutinin controls viral attachment and infection of host cells. This binding is a three-way interaction between viral proteins, host glycans, and viral glycans; many structural details of this interaction have been difficult to resolve.
Kasson, Peter M., Pande, Vijay S.
openaire   +2 more sources