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Tubulin and microtubule are potential targets for brain hexokinase binding [PDF]
FEBS Letters, 2001 The metabolite-modulated association of a fraction of hexokinase to mitochondria in brain is well documented, however, the involvement of other non-mitochondrial components in the binding of the hexokinase is controversial.
Peter Low, J Ovádi, F Orosz
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Human Hexokinase II: Sequence and Homology to Other Hexokinases
Biochemical and Biophysical Research Communications, 1993The amino acid sequence of human hexokinase II was deduced from the sequence of cDNA clones isolated from a skeletal muscle library. An open reading frame of 2751 bases encodes a protein of 917 amino acids. The deduced amino acid sequence has 94% identity with rat hexokinase II but only 72% identity with human hexokinase type I.
S S, Deeb, M, Malkki, M, Laakso
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Glucose oxidase/hexokinase electrode for the determination of ATP
Analytica Chimica Acta, 1997 A hydrogen peroxide based enzyme electrode for the determination of ATP has been developed by the immobilization of glucose oxidase and hexokinase. Competition between the enzymes for the substrate glucose allowed the measurement of ATP.
Dario Compagnone, George G Guilbault
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Hexokinase redistribution in vivo
Biochimica et Biophysica Acta (BBA) - General Subjects, 1990Heterogenous stock mice in addition to mice selectively bred to maximally differ in their severity of alcohol withdrawal seizures (withdrawal seizure-resistant (WSR) and withdrawal seizure-prone (WSP] were used to provide evidence in favor of the importance of the rapidly changing distribution of brain hexokinase (ATP: D-hexose 6-phosphotransferase, EC
S E, Laursen +3 more
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An ultrasensitive radioassay for hexokinase
Analytical Biochemistry, 1973Abstract A batch chromatographic method for the determination of hexokinase employing trace-labelled glucose and resin chromatography is described. Markedly enhanced sensitivity compared with the standard spectrophotometric assay is shown. Its greater reliability and use for particulate-tissue preparations is discussed.
R E, Gots, S P, Bessman
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Influence of some Inhibitors of Animal Hexokinase on Yeast Hexokinase
Nature, 1949THE observations of Colowick et al.1 that the hexokinase activity of extracts of brain or muscles from normal animals may be inhibited by the addition of some anterior pituitary extracts in the presence of adrenal cortex extract, and that this inhibitory effect is prevented by insulin, has been confirmed by Reid, Smith and Young2.
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The Preparation of Yeast Hexokinases
Preparative Biochemistry, 1971Abstract Improved methods are described for the preparation of hexokinase from baker's yeast. The isolation procedure is designed to avoid proteolysis, by using mechanical disintegration of the yeast cells, by organophosphate inhibition of the serine-dependent proteases, and by removal of all other proteases by gel filtration.
Y M, Rustum, A H, Ramel, E A, Barnard
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Redox Regulation of Hexokinases
Antioxidants & Redox Signaling, 2018Significance: Hexokinases are key enzymes that are responsible for the first reaction of glycolysis, but they also moonlight other cellular processes, including mitochondrial redox signaling regulation. Modulation of hexokinase activity and spatiotemporal location by reactive oxygen and nitrogen species as well ...
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Anomeric Specificity of Mammalian Hexokinase
Archives Internationales de Physiologie et de Biochimie, 1986The anomeric specificity of hexokinase was examined in crude homogenates of rat parotid gland, erythrocytes and pancreatic islets. At 8 degrees C, the alpha/beta ratio in maximal velocity averaged 0.73, 0.66 and 0.75 in the parotid, erythrocytes and pancreatic islets, respectively.
Giroix, Marie-Hélène +3 more
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Experimental Eye Research, 1973
Abstract Clear crystalline lenses were obtained post-mortem from human prenates, neonates, young and old adults. Senile cataracts removed surgically were also studied. The specific activity of soluble fetal lens hexokinase is equal to that of the rat lens. There is a progressive decrease in specific activity as the lens size increases.
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Abstract Clear crystalline lenses were obtained post-mortem from human prenates, neonates, young and old adults. Senile cataracts removed surgically were also studied. The specific activity of soluble fetal lens hexokinase is equal to that of the rat lens. There is a progressive decrease in specific activity as the lens size increases.
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