Results 11 to 20 of about 373,405 (315)

Single-nucleotide polymorphism discovery by high-throughput sequencing in sorghum [PDF]

BMC Genomics, 2011
Background Eight diverse sorghum (Sorghum bicolor L. Moench) accessions were subjected to short-read genome sequencing to characterize the distribution of single-nucleotide polymorphisms (SNPs).
White Frank F, Antony Ginny, Li Xianran, Wu Yuye, Wang Shichen, Nelson James C, Yu Jianming   +6 more
doaj   +3 more sources

Ten simple rules for managing high-throughput nucleotide sequencing data [PDF]

bioRxiv, 2016
The challenges posed by large data volumes produced by high-throughput nucleotide sequencing technologies are well known. This document establishes ten simple rules for coping with these challenges. At the level of master data management, (1) data triage
R. Vos
semanticscholar   +2 more sources

High-throughput sequencing reveals single nucleotide variants in longer-kernel bread wheat [PDF]

Frontiers in Plant Science, 2016
The transcriptomes of bread wheat Yunong 201 and its ethyl methanesulfonate (EMS) derivative Yunong 3114 were obtained by next-sequencing technology. Single nucleotide variants (SNVs) in the wheat strains were explored and compared.
Feng Chen, Zibo Zhu, Xiaobian Zhou, Yan Yan, Zhongdong Dong, Dangqun Cui   +5 more
doaj   +3 more sources

High-throughput nucleotide sequence analysis of diverse bacterial communities in leachates of decomposing pig carcasses

Genetics and Molecular Biology, 2015
The leachate generated by the decomposition of animal carcass has been implicated as an environmental contaminant surrounding the burial site. High-throughput nucleotide sequencing was conducted to investigate the bacterial communities in leachates from ...
Seung-Hak Yang, Joung-Soo Lim, Modabber Ahmed Khan, Bong-Soo Kim, D. Choi, Eun Young Lee, H. Ahn   +6 more
semanticscholar   +7 more sources

Uncommon nucleotide excision repair phenotypes revealed by targeted high-throughput sequencing [PDF]

Orphanet Journal of Rare Diseases, 2016
BackgroundDeficient nucleotide excision repair (NER) activity causes a variety of autosomal recessive diseases including xeroderma pigmentosum (XP) a disorder which pre-disposes to skin cancer, and the severe multisystem condition known as Cockayne ...
N. Calmels, G. Greff, C. Obringer, N. Kempf, C. Gasnier, J. Tarabeux, M. Miguet, G. Baujat, D. Bessis, P. Bretones, A. Cavau, B. Digeon, M. Doco‐Fenzy, B. Doray, F. Feillet, J. Gardeazabal, B. Gener, S. Julia, I. Llano-Rivas, A. Mazur, C. Michot, Florence Renaldo-Robin, M. Rossi, P. Sabouraud, B. Keren, C. Depienne, Jean Muller, J. Mandel, V. Laugel   +28 more
semanticscholar   +5 more sources

High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution

Nucleic Acids Research, 2018
Targeted PCR amplification and high-throughput sequencing (amplicon sequencing) of 16S rRNA gene fragments is widely used to profile microbial communities.
B. Callahan, J. Wong, C. Heiner, Steve Oh, C. Theriot, Ajay S. Gulati, Sarah K. McGill, Michael K. Dougherty   +7 more
semanticscholar   +3 more sources

ANNOVAR: functional annotation of genetic variants from high-throughput sequencing data

Nucleic Acids Research, 2010
High-throughput sequencing platforms are generating massive amounts of genetic variation data for diverse genomes, but it remains a challenge to pinpoint a small subset of functionally important variants.
Kai Wang, Mingyao Li, H. Hakonarson
semanticscholar   +3 more sources

Precision Medicine Based on High-Throughput Nucleotide Sequencing for a Patient with SCN2A-Related Developmental Epileptic Encephalopathy [PDF]

Annals of Child Neurology, 2020
(SCN2A) gene (OMIM 182390) encodes the alpha subunit of Nav1.2 [1]. It is expressed plentifully in neurons during early infancy and plays a role in neuronal excitability [2].
S. E. Park, Dajeong Lee, J. Song, Jeehun Lee, Jiwon Lee   +4 more
semanticscholar   +2 more sources

More affordable and effective noninvasive SNP genotyping using high-throughput amplicon sequencing [PDF]

Molecular Ecology Resources, 2019
Non-invasive genotyping methods have become key elements of wildlife research over the last two decades, but their widespread adoption is limited by high costs, low success rates, and high error rates.
Charlotte E. Eriksson, Joel S. Ruprecht, Taal Levi   +2 more
semanticscholar   +3 more sources

Large scale single nucleotide polymorphism discovery in unsequenced genomes using second generation high throughput sequencing technology: applied to turkey [PDF]

BMC Genomics, 2009
Background - The development of second generation sequencing methods has enabled large scale DNA variation studies at moderate cost. For the high throughput discovery of single nucleotide polymorphisms (SNPs) in species lacking a sequenced reference ...
Kerstens, H.H.D., Crooijmans, R.P.M.A., Veenendaal, A., Dibbits, B.W., Chin-A-Woeng, T.F.C., Dunnen, J.T., den, Groenen, M.A.M.   +6 more
core   +7 more sources