Results 241 to 250 of about 213,884 (275)

Recent advances in isothermal amplification techniques coupled with clustered regularly interspaced short palindromic repeat/Cas systems

Interdisciplinary Medicine, EarlyView.
Abstract Isothermal amplification (IA) techniques have emerged as promising alternatives to polymerase chain reaction (PCR), enabling rapid and efficient nucleic acid amplification at constant temperatures. Meanwhile, clustered regularly interspaced short palindromic repeat (CRISPR)/Cas‐based technology has revolutionized molecular diagnostics by ...
Miao Liu, Yajun Hou, Yuhang Cheng, Ziyun Li, Jiawei Zeng, Li Li, Jinyong Luo, Bo Shen   +7 more
wiley   +1 more source

HPTAS: An Alignment-Free Haplotype Phasing Algorithm Focused on Allele-Specific Studies Using Transcriptome Data. [PDF]

Int J Mol Sci
Wang J, Sun Z, Wang G, Miao Y.
europepmc   +1 more source

Advanced single‐cell RNA sequencing in tumor immunology

Interdisciplinary Medicine, EarlyView.
This review highlights the applications of advanced single‐cell RNA sequencing (scRNA‐seq) technologies in tumor immunology. It summarizes representative scRNA‐seq methods according to technical principles, with a focus on single‐cell T cell receptor sequencing. This review also discusses how scRNA‐seq is used to construct immune cell atlases of tumors,
Yilong Liu, Xiyuan Yu, Zhipeng Han, Qiuyang Wei, Qi Niu, Chaoyong Yang, Jie P. Li, Zijian Guo, Huimin Zhang   +8 more
wiley   +1 more source

EasyDIVER + : An Advanced Tool for Analyzing High Throughput Sequencing Data from In Vitro Evolution of Nucleic Acids or Amino Acids. [PDF]

J Mol Evol
Blanco C, Tee A, Sharma P, Newton MS, Lee KH, Erickson SE, Seelig B, Chen IA.   +7 more
europepmc   +1 more source

Optical Method for the Detection of Viral RNA Using an Optical Fiber Sensor

Journal of Biophotonics, EarlyView.
This study presents a fiber‐optic sensor with a microsphere tip functionalized with a gold layer and oligonucleotide probes for detecting SARS‐CoV‐2 RNA at concentrations as low as 10−12 M in near real time. ABSTRACT This study introduces a fiber‐optic sensor functionalized with a sensing probe for SARS‐CoV‐2 RNA detection.
Patryk Sokołowski, Paweł Wityk, Joanna Raczak‐Gutknecht, Wiktoria Brzezińska, Michał Sobaszek, Paweł Kalinowski, Sebastian Garcia‐Galan, Małgorzata Szczerska   +7 more
wiley   +1 more source

CRISPR/Cas9 Knockout of Shell Matrix Protein 1 in the Slipper‐Snail Crepidula atrasolea

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, EarlyView.
Methods for CRISPR/Cas9 shell matrix protein knockout in the snail, Crepidula atrasolea including optimizing RNP delivery and embryo husbandry, to the use of Sanger and MiSeq sequencing, and high‐content screening of embryos to characterize the loss of Shell Matrix Protein 1 (SMP1) during larval shell development.
Grant Batzel, Yiqun Wang, Antonia Bock, Elbereth Chen, Stephanie Neal, Rebecca N. Lopez‐Anido, Yoon Lee, Evan Tjeerdema, Emily Ignatoff, Tejasvi Patil, Gabriela Ramirez, Maryna P. Lesoway, Amro Hamdoun, Deirdre C. Lyons   +13 more
wiley   +1 more source

Ligase-catalyzed transcription and reverse-transcription of XNA-containing nucleic acid polymers using T3 DNA ligase. [PDF]

Chem Sci
Khamissi N, Korfmann C, Chaudhry A, Hili R.   +3 more
europepmc   +1 more source

Bridging lab and industry: The impact of a bio‐conversion unit on black soldier fly larvae production and microbiome dynamics

JSFA reports, EarlyView.
Abstract Background This study evaluates the viability of a novel bio‐conversion unit (BCU) for extensive black soldier fly larvae (BSFL) production as a sustainable feed additive for livestock. The BCU's effectiveness in converting organic byproducts into valuable biomass via the production of BSFL was assessed to reduce reliance on environmentally ...
William J. S. Edwards, Chloe Skingle, Richard Small, Robert Barker, Anastasios D. Tsaousis   +4 more
wiley   +1 more source

Next-generation sequencing: A powerful multi-purpose tool in cell line development for biologics production. [PDF]

Comput Struct Biotechnol J
Grassi L, Harris C, Zhu J, Hatton D, Dunn S.   +4 more
europepmc   +1 more source

Ecogenomics and limnological dynamics of a new Thiocapsa species blooming in the whole water column of a karstic lake

Limnology and Oceanography, EarlyView.
Abstract Purple sulfur bacteria (PSB) of the family Chromatiaceae (Gammaproteobacteria) can perform chemo‐ and photo‐lithoautotrophy (through anoxygenic photosynthesis) in anoxic layers of freshwater stratified (including meromictic) lakes. This group has been extensively studied via physiological and ecological approaches, albeit their genomics has ...
Pedro J. Cabello‐Yeves, Antonio Picazo, Carlos Rochera, Daniel Morant, Eduardo Vicente, David J. Scanlan, Richard J. Puxty, Antonio Camacho   +7 more
wiley   +1 more source