High-throughput sequencing: a failure mode analysis [PDF]
BMC Genomics, 2005 Background Basic manufacturing principles are becoming increasingly important in high-throughput sequencing facilities where there is a constant drive to increase quality, increase efficiency, and decrease operating costs.
Marra Marco A +6 more
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High-throughput single-сell sequencing in cancer research
Signal Transduction and Targeted Therapy, 2022 With advances in sequencing and instrument technology, bioinformatics analysis is being applied to batches of massive cells at single-cell resolution. High-throughput single-cell sequencing can be utilized for multi-omics characterization of tumor cells,
Qingzhu Jia +4 more
doaj +2 more sources
High Throughput Sequencing for Clinical Tuberculosis: An Overview
Pathogens, 2022 High throughput sequencing (HTS) can identify the presence of Mycobacterium tuberculosis DNA in a clinical sample while also providing information on drug susceptibility.
Tara E. Ness +2 more
doaj +3 more sources
Microfluidics applications for high-throughput single cell sequencing
Journal of Nanobiotechnology, 2021 The inherent heterogeneity of individual cells in cell populations plays significant roles in disease development and progression, which is critical for disease diagnosis and treatment.
Wen-min Zhou +10 more
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Macrophage Changes and High‐Throughput Sequencing in Aging Mouse Intervertebral Disks [PDF]
JOR SpineBackground Intervertebral disk (IVD) degeneration is associated with lower back pain and aging; however, the mechanisms underlying age‐related changes and the changes in macrophage polarization in aging intervertebral disks require further elucidation ...
Wei Wang +7 more
doaj +2 more sources
MeStanG—Resource for High-Throughput Sequencing Standard Data Sets Generation for Bioinformatic Methods Evaluation and Validation [PDF]
BiologyMetagenomics analysis has enabled the measurement of the microbiome diversity in environmental samples without prior targeted enrichment. Functional and phylogenetic studies based on microbial diversity retrieved using HTS platforms have advanced from ...
Daniel Ramos Lopez +2 more
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Analysing high-throughput sequencing data in Python with HTSeq 2.0 [PDF]
Bioinform., 2021 Summary HTSeq 2.0 provides a more extensive application programming interface including a new representation for sparse genomic data, enhancements for htseq-count to suit single-cell omics, a new script for data using cell and molecular barcodes ...
Givanna H. Putri +4 more
semanticscholar +1 more source
High-throughput Sequencing Technology and Its Application in Epigenetics Studies [PDF]
SHS Web of Conferences, 2023 DNA sequencing technology is one of the important tools in modern life science research, and with the development of science and technology, sequencing technology is constantly changing.
Wang Zexuan
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High-throughput RNA isoform sequencing using programmed cDNA concatenation.
Nature Biotechnology, 2023 Full-length RNA-sequencing methods using long-read technologies can capture complete transcript isoforms, but their throughput is limited. We introduce multiplexed arrays isoform sequencing (MAS-ISO-seq), a technique for programmably concatenating ...
Aziz M Al'Khafaji +18 more
semanticscholar +1 more source
Large scale library generation for high throughput sequencing. [PDF]
PLoS ONE, 2011 BACKGROUND: Large efforts have recently been made to automate the sample preparation protocols for massively parallel sequencing in order to match the increasing instrument throughput.
Erik Borgström +2 more
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