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High-throughput sequencing in vaccine research [PDF]

open access: yesJournal of Veterinary Research, 2021
The world of vaccines has changed tremendously since the time of Louis Pasteur. In the present day, it is regarded as vaccinology, a discipline which includes not only the knowledge of vaccine production, strategies for its delivery and influence on the ...
Pasik Katarzyna   +1 more
doaj   +3 more sources

AlmostSignificant: simplifying quality control of high-throughput sequencing data [PDF]

open access: hybrid, 2016
Motivation: The current generation of DNA sequencing technologies produce a large amount of data quickly. All of these data need to pass some form of quality control (QC) processing and checking before they can be used for any analysis.
Joseph Ward   +3 more
openalex   +8 more sources

High-throughput single-сell sequencing in cancer research

open access: yesSignal Transduction and Targeted Therapy, 2022
With advances in sequencing and instrument technology, bioinformatics analysis is being applied to batches of massive cells at single-cell resolution. High-throughput single-cell sequencing can be utilized for multi-omics characterization of tumor cells,
Qingzhu Jia   +4 more
doaj   +2 more sources

High throughput error corrected Nanopore single cell transcriptome sequencing

open access: yesNature Communications, 2020
Droplet-based high throughput single cell sequencing techniques can often lose information on transcript splicing and heterogenity. Here the authors introduce ScNaUmi-seq, which uses Oxford Nanopore sequencing and barcoding to generate high accuracy full
Kevin Lebrigand   +3 more
doaj   +2 more sources

Microfluidics applications for high-throughput single cell sequencing

open access: yesJournal of Nanobiotechnology, 2021
The inherent heterogeneity of individual cells in cell populations plays significant roles in disease development and progression, which is critical for disease diagnosis and treatment.
Wen-min Zhou   +10 more
doaj   +2 more sources

QIIME allows analysis of high-throughput community sequencing data

open access: yesNature Methods, 2010
J Gregory Caporaso   +2 more
exaly   +2 more sources

Analysing high-throughput sequencing data in Python with HTSeq 2.0 [PDF]

open access: yesBioinform., 2021
Summary HTSeq 2.0 provides a more extensive application programming interface including a new representation for sparse genomic data, enhancements for htseq-count to suit single-cell omics, a new script for data using cell and molecular barcodes ...
Givanna H. Putri   +4 more
semanticscholar   +1 more source

High Throughput Sequencing for Clinical Tuberculosis: An Overview

open access: yesPathogens, 2022
High throughput sequencing (HTS) can identify the presence of Mycobacterium tuberculosis DNA in a clinical sample while also providing information on drug susceptibility.
Tara E. Ness   +2 more
doaj   +1 more source

HTSlib: C library for reading/writing high-throughput sequencing data

open access: yesGigaScience, 2021
Background Since the original publication of the VCF and SAM formats, an explosion of software tools have been created to process these data files. To facilitate this a library was produced out of the original SAMtools implementation, with a focus on ...
J. Bonfield   +7 more
semanticscholar   +1 more source

Large scale library generation for high throughput sequencing. [PDF]

open access: yesPLoS ONE, 2011
BACKGROUND: Large efforts have recently been made to automate the sample preparation protocols for massively parallel sequencing in order to match the increasing instrument throughput.
Erik Borgström   +2 more
doaj   +1 more source

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