Results 21 to 30 of about 308,924 (265)

High‐Throughput Multiplex Sequencing of miRNA [PDF]

Current Protocols in Human Genetics, 2012
AbstractNext‐generation sequencing offers many advantages over other methods of microRNA (miRNA) expression profiling, such as sample throughput and the capability to discover novel miRNAs. As the sequencing depth of current sequencing platforms exceeds what is necessary to quantify miRNAs, multiplexing several samples in one sequencing run offers a ...
Francois, Vigneault, Dmitry, Ter-Ovanesyan, Shahar, Alon, Seda, Eminaga, Danos, C Christodoulou, J G, Seidman, Eli, Eisenberg, George, M Church   +7 more
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SnapShot: High-Throughput Sequencing Applications

Cell, 2011
/ 25 (X2) 8 daysPacific Biosciences http://www.pacificbiosciences.comNo amplification; Synthesis Fluorescence >1000 bp (SE) 150,000 per SMRT cell N/AIon Torrent http://www.iontorrent.comEmulsion PCR; Synthesis Change in pH 200 bp (SE) Variable 90% of the full genome resolved (both alleles)400 human genomes per monthModified and updated from Metzker ...
Han, Hong, Nutiu, Razvan, Moffat, Jason, Blencowe, Benjamin J.   +3 more
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Ion torrent high throughput mitochondrial genome sequencing (HTMGS).

PLoS ONE, 2019
The implementation and popularity of next generation sequencing (NGS) has led to the development of various rapid whole mitochondrial genome sequencing techniques. We summarise an efficient and cost-effective NGS approach for mitochondrial genomic DNA in
N R Harvey, C L Albury, S Stuart, M C Benton, D A Eccles, J R Connell, H G Sutherland, R J N Allcock, R A Lea, L M Haupt, L R Griffiths   +10 more
doaj   +1 more source

First occurrence of bidens mottle virus in Brazil: biological and molecular characterization of isolates infecting Zinnia sp. and Bidens pilosa [PDF]

Scientia Agricola, 2023
Zinnia sp. and hairy beggartick (Bidens pilosa) plants exhibiting symptoms of possible virus infection were found in the municipality of Santa Bárbara d’Oeste, São Paulo State, Brazil.
Gabriel Madoglio Favara, Camila Geovana Ferro, Vinicius Henrique Bello, Felipe Franco de Oliveira, Heron Delgado Kraide, Marcos Roberto Ribeiro Junior, Renate Krause-Sakate, Elliot Watanabe Kitajima, Jorge Alberto Marques Rezende   +8 more
doaj   +1 more source

Targeted high throughput sequencing in hereditary ataxia and spastic paraplegia. [PDF]

PLoS ONE, 2017
Hereditary ataxia and spastic paraplegia are heterogeneous monogenic neurodegenerative disorders. To date, a large number of individuals with such disorders remain undiagnosed.
Zafar Iqbal, Siri L Rydning, Iselin M Wedding, Jeanette Koht, Lasse Pihlstrøm, Aina H Rengmark, Sandra P Henriksen, Chantal M E Tallaksen, Mathias Toft   +8 more
doaj   +1 more source

Small molecule regulators of microRNAs identified by high-throughput screen coupled with high-throughput sequencing

Nature Communications, 2023
MicroRNAs (miRNAs) regulate fundamental biological processes by silencing mRNA targets and are dysregulated in many diseases. Therefore, miRNA replacement or inhibition can be harnessed as potential therapeutics.
Lien D. Nguyen, Zhiyun Wei, M. Catarina Silva, Sergio Barberán-Soler, Jiarui Zhang, Rosalia Rabinovsky, Christina R. Muratore, Jonathan M. S. Stricker, Colin Hortman, Tracy L. Young-Pearse, Stephen J. Haggarty, Anna M. Krichevsky   +11 more
doaj   +1 more source

High-throughput single-сell sequencing in cancer research

Signal Transduction and Targeted Therapy, 2022
With advances in sequencing and instrument technology, bioinformatics analysis is being applied to batches of massive cells at single-cell resolution. High-throughput single-cell sequencing can be utilized for multi-omics characterization of tumor cells,
Qingzhu Jia, Han Chu, Zheng Jin, Haixia Long, Bo Zhu   +4 more
doaj   +1 more source

High Throughput Sequencing: An Overview of Sequencing Chemistry [PDF]

Indian Journal of Microbiology, 2016
In the present century sequencing is to the DNA science, what gel electrophoresis was to it in the last century. From 1977 to 2016 three generation of the sequencing technologies of various types have been developed. Second and third generation sequencing technologies referred commonly to as next generation sequencing technology, has evolved ...
Sheetal, Ambardar, Rikita, Gupta, Deepika, Trakroo, Rup, Lal, Jyoti, Vakhlu   +4 more
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High-throughput sequencing of a South American Amerindian. [PDF]

PLoS ONE, 2013
The emergence of next-generation sequencing technologies allowed access to the vast amounts of information that are contained in the human genome. This information has contributed to the understanding of individual and population-based variability and ...
André M Ribeiro-dos-Santos, Jorge Estefano Santana de Souza, Renan Almeida, Dayse O Alencar, Maria Silvanira Barbosa, Leonor Gusmão, Wilson A Silva, Sandro J de Souza, Artur Silva, Ândrea Ribeiro-dos-Santos, Sylvain Darnet, Sidney Santos   +11 more
doaj   +1 more source

High-Throughput Sequencing and Rare Genetic Diseases [PDF]

Molecular Syndromology, 2012
High-throughput sequencing has drastically changed the research of genes responsible for genetic disorders and is now gradually introduced as an additional genetic diagnostic testing in clinical practice. The current debates on the emerging technical, medical and ethical issues as well as the potential optimum use of the available technology are ...
Makrythanasis P, Antonarakis SE.
openaire   +3 more sources