Results 31 to 40 of about 1,074,205 (395)

YAMAT-seq: an efficient method for high-throughput sequencing of mature transfer RNAs. [PDF]

open access: yes, 2017
Besides translation, transfer RNAs (tRNAs) play many non-canonical roles in various biological pathways and exhibit highly variable expression profiles.
Honda, Shozo   +5 more
core   +2 more sources

High-throughput total RNA sequencing in single cells using VASA-seq

open access: yesNature Biotechnology, 2022
Most methods for single-cell transcriptome sequencing amplify the termini of polyadenylated transcripts, capturing only a small fraction of the total cellular transcriptome.
Fredrik Salmén   +15 more
semanticscholar   +1 more source

Qualimap 2: advanced multi-sample quality control for high-throughput sequencing data

open access: yesBioinform., 2015
Motivation: Detection of random errors and systematic biases is a crucial step of a robust pipeline for processing high-throughput sequencing (HTS) data.
K. Okonechnikov   +2 more
semanticscholar   +1 more source

High-Throughput Microdissection for Next-Generation Sequencing. [PDF]

open access: yesPLoS ONE, 2016
Precision medicine promises to enhance patient treatment through the use of emerging molecular technologies, including genomics, transcriptomics, and proteomics.
Avi Z Rosenberg   +17 more
doaj   +1 more source

Application of Nanopore Sequencing for High Throughput Genotyping in Horses

open access: yesAnimals, 2023
Nanopore sequencing is a third-generation biopolymer sequencing technique that relies on monitoring the changes in an electrical current that occur as nucleic acids are passed through a protein nanopore.
Artur Gurgul   +6 more
doaj   +1 more source

SOAPnuke: a MapReduce acceleration-supported software for integrated quality control and preprocessing of high-throughput sequencing data

open access: yesGigaScience, 2017
Quality control (QC) and preprocessing are essential steps for sequencing data analysis to ensure the accuracy of results. However, existing tools cannot provide a satisfying solution with integrated comprehensive functions, proper architectures, and ...
Yuxin Chen   +14 more
semanticscholar   +1 more source

Perspectives and Benefits of High-Throughput Long-Read Sequencing in Microbial Ecology

open access: yesApplied and Environmental Microbiology, 2021
Short-read, high-throughput sequencing (HTS) methods have yielded numerous important insights into microbial ecology and function. Yet, in many instances short-read HTS techniques are suboptimal, for example, by providing insufficient phylogenetic ...
L. Tedersoo   +3 more
semanticscholar   +1 more source

High‐Throughput Multiplex Sequencing of miRNA [PDF]

open access: yesCurrent Protocols in Human Genetics, 2012
AbstractNext‐generation sequencing offers many advantages over other methods of microRNA (miRNA) expression profiling, such as sample throughput and the capability to discover novel miRNAs. As the sequencing depth of current sequencing platforms exceeds what is necessary to quantify miRNAs, multiplexing several samples in one sequencing run offers a ...
Francois, Vigneault   +7 more
openaire   +2 more sources

High Throughput Sequencing for Detection of Foodborne Pathogens

open access: yesFrontiers in Microbiology, 2017
High-throughput sequencing (HTS) is becoming the state-of-the-art technology for typing of microbial isolates, especially in clinical samples. Yet, its application is still in its infancy for monitoring and outbreak investigations of foods.
Camilla Sekse   +6 more
doaj   +1 more source

High-throughput DNA sequencing to survey bacterial histidine and tyrosine decarboxylases in raw milk cheeses [PDF]

open access: yes, 2015
peer-reviewedBackground The aim of this study was to employ high-throughput DNA sequencing to assess the incidence of bacteria with biogenic amine (BA; histamine and tyramine) producing potential from among 10 different cheeses varieties.
Cotter, Paul D.   +6 more
core   +1 more source

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