High Throughput Sequencing of Extracellular RNA from Human Plasma. [PDF]
PLoS ONE, 2017 The presence and relative stability of extracellular RNAs (exRNAs) in biofluids has led to an emerging recognition of their promise as 'liquid biopsies' for diseases.
Kirsty M Danielson +4 more
doaj +1 more source
High-throughput total RNA sequencing in single cells using VASA-seq
Nature Biotechnology, 2022 Most methods for single-cell transcriptome sequencing amplify the termini of polyadenylated transcripts, capturing only a small fraction of the total cellular transcriptome.
Fredrik Salmén +15 more
semanticscholar +1 more source
HTSeq—a Python framework to work with high-throughput sequencing data
bioRxiv, 2014 Motivation: A large choice of tools exists for many standard tasks in the analysis of high-throughput sequencing (HTS) data. However, once a project deviates from standard work flows, custom scripts are needed. Results: We present HTSeq, a Python library
S. Anders, P. Pyl, W. Huber
semanticscholar +1 more source
High‐Throughput Multiplex Sequencing of miRNA [PDF]
Current Protocols in Human Genetics, 2012 AbstractNext‐generation sequencing offers many advantages over other methods of microRNA (miRNA) expression profiling, such as sample throughput and the capability to discover novel miRNAs. As the sequencing depth of current sequencing platforms exceeds what is necessary to quantify miRNAs, multiplexing several samples in one sequencing run offers a ...
George M. Church +11 more
openaire +3 more sources
Endogenous RNAs Modulate MicroRNA Sorting to Exosomes and Transfer to Acceptor Cells
Cell Reports, 2014 MicroRNA (miRNA) transfer via exosomes may mediate cell-to-cell communication. Interestingly, specific miRNAs are enriched in exosomes in a cell-type-dependent fashion. However, the mechanisms whereby miRNAs are sorted to exosomes and the significance of
Mario Leonardo Squadrito +7 more
doaj +1 more source
Phage ImmunoPrecipitation Sequencing (PhIP-Seq): The Promise of High Throughput Serology
Pathogens, 2022 Phage ImmunoPrecipitation Sequencing (PhIP-Seq) is a high throughput serological technology that is revolutionizing the manner in which we track antibody profiles. In this review, we mainly focus on its application to viral infectious diseases.
Charles Kevin Tiu +3 more
doaj +1 more source
ANNOVAR: functional annotation of genetic variants from high-throughput sequencing data
Nucleic Acids Research, 2010 High-throughput sequencing platforms are generating massive amounts of genetic variation data for diverse genomes, but it remains a challenge to pinpoint a small subset of functionally important variants.
Kai Wang, Mingyao Li, H. Hakonarson
semanticscholar +1 more source
High throughput error corrected Nanopore single cell transcriptome sequencing
Nature Communications, 2020 Droplet-based high throughput single cell sequencing techniques can often lose information on transcript splicing and heterogenity. Here the authors introduce ScNaUmi-seq, which uses Oxford Nanopore sequencing and barcoding to generate high accuracy full
Kevin Lebrigand +3 more
doaj +1 more source
High Throughput Sequencing for the Detection and Characterization of RNA Viruses
Frontiers in Microbiology, 2021 This review aims to assess and recommend approaches for targeted and agnostic High Throughput Sequencing of RNA viruses in a variety of sample matrices.
Amy H. Fitzpatrick +5 more
semanticscholar +1 more source
Application of Nanopore Sequencing for High Throughput Genotyping in Horses
Animals, 2023 Nanopore sequencing is a third-generation biopolymer sequencing technique that relies on monitoring the changes in an electrical current that occur as nucleic acids are passed through a protein nanopore.
Artur Gurgul +6 more
doaj +1 more source