First occurrence of bidens mottle virus in Brazil: biological and molecular characterization of isolates infecting Zinnia sp. and Bidens pilosa [PDF]
Zinnia sp. and hairy beggartick (Bidens pilosa) plants exhibiting symptoms of possible virus infection were found in the municipality of Santa Bárbara d’Oeste, São Paulo State, Brazil.
Gabriel Madoglio Favara +8 more
doaj +1 more source
High throughput genomic sequencing of bioaerosols in broiler chicken production facilities [PDF]
Chronic inhalation exposure to agricultural dust promotes the development of chronic respiratory diseases among poultry workers. Poultry dust is composed of dander, chicken feed, litter bedding and microbes.
Bair, Thomas +6 more
core +2 more sources
High Throughput Sequencing for the Detection and Characterization of RNA Viruses
This review aims to assess and recommend approaches for targeted and agnostic High Throughput Sequencing of RNA viruses in a variety of sample matrices.
Amy H. Fitzpatrick +5 more
semanticscholar +1 more source
SnapShot: High-Throughput Sequencing Applications
/ 25 (X2) 8 daysPacific Biosciences http://www.pacificbiosciences.comNo amplification; Synthesis Fluorescence >1000 bp (SE) 150,000 per SMRT cell N/AIon Torrent http://www.iontorrent.comEmulsion PCR; Synthesis Change in pH 200 bp (SE) Variable 90% of the full genome resolved (both alleles)400 human genomes per monthModified and updated from Metzker ...
Han, Hong +3 more
openaire +2 more sources
Ion torrent high throughput mitochondrial genome sequencing (HTMGS).
The implementation and popularity of next generation sequencing (NGS) has led to the development of various rapid whole mitochondrial genome sequencing techniques. We summarise an efficient and cost-effective NGS approach for mitochondrial genomic DNA in
N R Harvey +10 more
doaj +1 more source
Effects of propidium monoazide (PMA) treatment on mycobiome and bacteriome analysis of cystic fibrosis airways during exacerbation [PDF]
Introduction and Purpose : Propidium monoazide (PMA)-pretreatment has increasingly been applied to remove the bias from dead or damaged cell artefacts, which could impact the microbiota analysis by high-throughput sequencing. Our study aimed to determine
Audebert, Christophe +8 more
core +3 more sources
Base calling for high-throughput short-read sequencing: dynamic programming solutions [PDF]
Shreepriya Das and Haris Vikalo are with the Electrical and Computer Engineering Department, The University of Texas at Austin, Austin, Texas 78712, USABackground: Next-generation DNA sequencing platforms are capable of generating millions of reads in ...
Das, Shreepriya, Vikalo, Haris
core +3 more sources
HaploGrep 2: mitochondrial haplogroup classification in the era of high-throughput sequencing
Mitochondrial DNA (mtDNA) profiles can be classified into phylogenetic clusters (haplogroups), which is of great relevance for evolutionary, forensic and medical genetics.
H. Weissensteiner +8 more
semanticscholar +1 more source
Targeted high throughput sequencing in hereditary ataxia and spastic paraplegia. [PDF]
Hereditary ataxia and spastic paraplegia are heterogeneous monogenic neurodegenerative disorders. To date, a large number of individuals with such disorders remain undiagnosed.
Zafar Iqbal +8 more
doaj +1 more source
Experimental designs that take advantage of high-throughput sequencing to generate datasets include RNA sequencing (RNA-seq), chromatin immunoprecipitation sequencing (ChIP-seq), sequencing of 16S rRNA gene fragments, metagenomic analysis and selective ...
A. Fernandes +5 more
semanticscholar +1 more source

