Results 71 to 80 of about 1,036,014 (384)

ReSeq simulates realistic Illumina high-throughput sequencing data

Genome Biology, 2021
In high-throughput sequencing data, performance comparisons between computational tools are essential for making informed decisions at each step of a project.
Stephan Schmeing, Mark D. Robinson
doaj   +1 more source

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing. [PDF]

, 2018
Sequencing technologies have undergone a paradigm shift from bulk to single-cell resolution in response to an evolving understanding of the role of cellular heterogeneity in biological systems.
Abate, Adam R, Demaree, Benjamin, Lan, Freeman, Weisgerber, Daniel   +3 more
core   +2 more sources

Efficient COI barcoding using high throughput single-end 400 bp sequencing

BMC Genomics, 2020
Background Over the last decade, the rapid development of high-throughput sequencing platforms has accelerated species description and assisted morphological classification through DNA barcoding. However, the current high-throughput DNA barcoding methods
Chentao Yang, Yuxuan Zheng, Shangjin Tan, Guanliang Meng, Wei Rao, Caiqing Yang, David G. Bourne, Paul A. O’Brien, Junqiang Xu, Sha Liao, Ao Chen, Xiaowei Chen, Xinrui Jia, Ai-bing Zhang, Shanlin Liu   +14 more
doaj   +1 more source

High-throughput sequencing of the transcriptome and chromatin accessibility in the same cell

Nature Biotechnology, 2019
Single-cell RNA sequencing can reveal the transcriptional state of cells, yet provides little insight into the upstream regulatory landscape associated with open or accessible chromatin regions.
S. Chen, B. Lake, Kun Zhang
semanticscholar   +1 more source

Fluorescent amplification for next generation sequencing (FA-NGS) library preparation. [PDF]

, 2020
BACKGROUND:Next generation sequencing (NGS) has become a universal practice in modern molecular biology. As the throughput of sequencing experiments increases, the preparation of conventional multiplexed libraries becomes more labor intensive ...
Chiniquy, Jennifer, Garber, Megan E, Hillson, Nathan J, Mukhopadhyay, Aindrila   +3 more
core   +1 more source

Breaking Barriers with Bread: Using the Sourdough Starter Microbiome to Teach High-Throughput Sequencing Techniques

Journal of Microbiology & Biology Education, 2022
Widespread usage of high-throughput sequencing (HTS) in the LIFE SCIENCES has produced a demand for undergraduate and graduate institutions to offer classes exposing students to all aspects of HTS (sample acquisition, laboratory work, sequencing ...
Benjamin H. Holt, Alison Buchan, Jennifer M. DeBruyn, Heidi Goodrich-Blair, Elizabeth McPherson, Veronica A. Brown   +5 more
doaj   +1 more source

FoxO1 signaling in B cell malignancies and its therapeutic targeting

FEBS Letters, EarlyView.
FoxO1 has context‐specific tumor suppressor or oncogenic character in myeloid and B cell malignancies. This includes tumor‐promoting properties such as stemness maintenance and DNA damage tolerance in acute leukemias, or regulation of cell proliferation and survival, or migration in mature B cell malignancies.
Krystof Hlavac, Petra Pavelkova, Laura Ondrisova, Marek Mraz   +3 more
wiley   +1 more source

Robust Sub-nanomolar Library Preparation for High Throughput Next Generation Sequencing

BMC Genomics, 2018
Background Current library preparation protocols for Illumina HiSeq and MiSeq DNA sequencers require ≥2 nM initial library for subsequent loading of denatured cDNA onto flow cells.
Wells W. Wu, Je-Nie Phue, Chun-Ting Lee, Changyi Lin, Lai Xu, Rong Wang, Yaqin Zhang, Rong-Fong Shen   +7 more
doaj   +1 more source

LoFreq: a sequence-quality aware, ultra-sensitive variant caller for uncovering cell-population heterogeneity from high-throughput sequencing datasets

Nucleic Acids Research, 2012
The study of cell-population heterogeneity in a range of biological systems, from viruses to bacterial isolates to tumor samples, has been transformed by recent advances in sequencing throughput. While the high-coverage afforded can be used, in principle,
A. Wilm, P. Aw, D. Bertrand, G. Yeo, S. Ong, Chang Hua Wong, C. Khor, R. Petric, M. Hibberd, N. Nagarajan   +9 more
semanticscholar   +1 more source

High-throughput sequencing reveals a simple model of nucleosome energetics

, 2010
We use nucleosome maps obtained by high-throughput sequencing to study sequence specificity of intrinsic histone-DNA interactions. In contrast with previous approaches, we employ an analogy between a classical one-dimensional fluid of finite-size ...
A. V. Morozov, Adams, D. Tolkunov, Deckert, Fedor, Felsenfeld, Field, G. Locke, Horz, Ioshikhes, Jenuwein, K. Struhl, Kaplan, Kornberg, Lee, Mavrich, Miller, Richmond, Segal, Sekinger, Tillo, Tolstorukov, Wang, Widom, Wingert, Yuan, Z. Moqtaderi   +26 more
core   +1 more source