Results 11 to 20 of about 321,953 (262)

Rapid genotyping of soybean cultivars using high throughput sequencing. [PDF]

open access: yesPLoS ONE, 2011
Soybean (Glycine max) breeding involves improving commercially grown varieties by introgressing important agronomic traits from poor yielding accessions and/or wild relatives of soybean while minimizing the associated yield drag.
Kranthi Varala   +3 more
doaj   +1 more source

High Throughput Sequencing: An Overview of Sequencing Chemistry [PDF]

open access: yesIndian Journal of Microbiology, 2016
In the present century sequencing is to the DNA science, what gel electrophoresis was to it in the last century. From 1977 to 2016 three generation of the sequencing technologies of various types have been developed. Second and third generation sequencing technologies referred commonly to as next generation sequencing technology, has evolved ...
Sheetal, Ambardar   +4 more
openaire   +2 more sources

High‐throughput sequencing of the melanoma genome [PDF]

open access: yesExperimental Dermatology, 2012
AbstractNext‐generation sequencing technologies are now common for whole‐genome, whole‐exome and whole‐transcriptome sequencing (RNA‐seq) of tumors to identify point mutations, structural or copy number alterations and changes in gene expression. A substantial number of studies have already been performed for melanoma. One study analysed eight melanoma
Manfred, Kunz   +2 more
openaire   +2 more sources

High Throughput Sequencing of Extracellular RNA from Human Plasma. [PDF]

open access: yesPLoS ONE, 2017
The presence and relative stability of extracellular RNAs (exRNAs) in biofluids has led to an emerging recognition of their promise as 'liquid biopsies' for diseases.
Kirsty M Danielson   +4 more
doaj   +1 more source

High‐Throughput Multiplex Sequencing of miRNA [PDF]

open access: yesCurrent Protocols in Human Genetics, 2012
AbstractNext‐generation sequencing offers many advantages over other methods of microRNA (miRNA) expression profiling, such as sample throughput and the capability to discover novel miRNAs. As the sequencing depth of current sequencing platforms exceeds what is necessary to quantify miRNAs, multiplexing several samples in one sequencing run offers a ...
Francois, Vigneault   +7 more
openaire   +2 more sources

Endogenous RNAs Modulate MicroRNA Sorting to Exosomes and Transfer to Acceptor Cells

open access: yesCell Reports, 2014
MicroRNA (miRNA) transfer via exosomes may mediate cell-to-cell communication. Interestingly, specific miRNAs are enriched in exosomes in a cell-type-dependent fashion. However, the mechanisms whereby miRNAs are sorted to exosomes and the significance of
Mario Leonardo Squadrito   +7 more
doaj   +1 more source

Phage ImmunoPrecipitation Sequencing (PhIP-Seq): The Promise of High Throughput Serology

open access: yesPathogens, 2022
Phage ImmunoPrecipitation Sequencing (PhIP-Seq) is a high throughput serological technology that is revolutionizing the manner in which we track antibody profiles. In this review, we mainly focus on its application to viral infectious diseases.
Charles Kevin Tiu   +3 more
doaj   +1 more source

High throughput error corrected Nanopore single cell transcriptome sequencing

open access: yesNature Communications, 2020
Droplet-based high throughput single cell sequencing techniques can often lose information on transcript splicing and heterogenity. Here the authors introduce ScNaUmi-seq, which uses Oxford Nanopore sequencing and barcoding to generate high accuracy full
Kevin Lebrigand   +3 more
doaj   +1 more source

Application of Nanopore Sequencing for High Throughput Genotyping in Horses

open access: yesAnimals, 2023
Nanopore sequencing is a third-generation biopolymer sequencing technique that relies on monitoring the changes in an electrical current that occur as nucleic acids are passed through a protein nanopore.
Artur Gurgul   +6 more
doaj   +1 more source

High-Throughput Microdissection for Next-Generation Sequencing. [PDF]

open access: yesPLoS ONE, 2016
Precision medicine promises to enhance patient treatment through the use of emerging molecular technologies, including genomics, transcriptomics, and proteomics.
Avi Z Rosenberg   +17 more
doaj   +1 more source

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