Results 31 to 40 of about 321,953 (262)

Scrutinizing virus genome termini by high-throughput sequencing. [PDF]

PLoS ONE, 2014
Analysis of genomic terminal sequences has been a major step in studies on viral DNA replication and packaging mechanisms. However, traditional methods to study genome termini are challenging due to the time-consuming protocols and their inefficiency ...
Shasha Li, Hang Fan, Xiaoping An, Huahao Fan, Huanhuan Jiang, Yubao Chen, Yigang Tong   +6 more
doaj   +1 more source

High-throughput sequencing of a South American Amerindian. [PDF]

PLoS ONE, 2013
The emergence of next-generation sequencing technologies allowed access to the vast amounts of information that are contained in the human genome. This information has contributed to the understanding of individual and population-based variability and ...
André M Ribeiro-dos-Santos, Jorge Estefano Santana de Souza, Renan Almeida, Dayse O Alencar, Maria Silvanira Barbosa, Leonor Gusmão, Wilson A Silva, Sandro J de Souza, Artur Silva, Ândrea Ribeiro-dos-Santos, Sylvain Darnet, Sidney Santos   +11 more
doaj   +1 more source

High-throughput sequencing: a failure mode analysis

BMC Genomics, 2005
Background Basic manufacturing principles are becoming increasingly important in high-throughput sequencing facilities where there is a constant drive to increase quality, increase efficiency, and decrease operating costs.
Marra Marco A, Balasundaram Miruna, Barber Sarah A, Smailus Duane, Stott Jeffery M, Yang George S, Holt Robert A   +6 more
doaj   +1 more source

Efficient COI barcoding using high throughput single-end 400 bp sequencing

BMC Genomics, 2020
Background Over the last decade, the rapid development of high-throughput sequencing platforms has accelerated species description and assisted morphological classification through DNA barcoding. However, the current high-throughput DNA barcoding methods
Chentao Yang, Yuxuan Zheng, Shangjin Tan, Guanliang Meng, Wei Rao, Caiqing Yang, David G. Bourne, Paul A. O’Brien, Junqiang Xu, Sha Liao, Ao Chen, Xiaowei Chen, Xinrui Jia, Ai-bing Zhang, Shanlin Liu   +14 more
doaj   +1 more source

ReSeq simulates realistic Illumina high-throughput sequencing data

Genome Biology, 2021
In high-throughput sequencing data, performance comparisons between computational tools are essential for making informed decisions at each step of a project.
Stephan Schmeing, Mark D. Robinson
doaj   +1 more source

SnapShot: High-Throughput Sequencing Applications

Cell, 2011
/ 25 (X2) 8 daysPacific Biosciences http://www.pacificbiosciences.comNo amplification; Synthesis Fluorescence >1000 bp (SE) 150,000 per SMRT cell N/AIon Torrent http://www.iontorrent.comEmulsion PCR; Synthesis Change in pH 200 bp (SE) Variable 90% of the full genome resolved (both alleles)400 human genomes per monthModified and updated from Metzker ...
Han, Hong, Nutiu, Razvan, Moffat, Jason, Blencowe, Benjamin J.   +3 more
openaire   +2 more sources

Breaking Barriers with Bread: Using the Sourdough Starter Microbiome to Teach High-Throughput Sequencing Techniques

Journal of Microbiology & Biology Education, 2022
Widespread usage of high-throughput sequencing (HTS) in the LIFE SCIENCES has produced a demand for undergraduate and graduate institutions to offer classes exposing students to all aspects of HTS (sample acquisition, laboratory work, sequencing ...
Benjamin H. Holt, Alison Buchan, Jennifer M. DeBruyn, Heidi Goodrich-Blair, Elizabeth McPherson, Veronica A. Brown   +5 more
doaj   +1 more source

Transcriptome-Wide High-Throughput m6A Sequencing of Differential m6A Methylation Patterns in the Human Rheumatoid Arthritis Fibroblast-Like Synoviocytes Cell Line MH7A

Journal of Inflammation Research, 2021
Hui Jiang1,2 *,* Kefeng Cao3 *,* Chang Fan,1,2 Xiaoya Cui,1,2 Yanzhen Ma,1,2 Jian Liu1 1Experimental Center of Clinical Research, First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui, People’s Republic of China; 2School ...
Jiang H, Cao K, Fan C, Cui X, Ma Y, Liu J   +5 more
doaj  

Phigaro: high-throughput prophage sequence annotation [PDF]

Bioinformatics, 2020
Abstract Summary Phigaro is a standalone command-line application that is able to detect prophage regions taking raw genome and metagenome assemblies as an input. It also produces dynamic annotated ‘prophage genome maps’ and marks possible transposon insertion spots inside prophages.
Elizaveta V. Starikova, Polina O. Tikhonova, Nikita A. Prianichnikov, Chris M. Rands, Evgeny M. Zdobnov, Elena N. Ilina, Vadim M. Govorun   +6 more
openaire   +2 more sources

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source