Reliable and accurate diagnostics from highly multiplexed sequencing assays [PDF]
, 2020 Scalable, inexpensive, and secure testing for SARS-CoV-2 infection is crucial for control of the novel coronavirus pandemic. Recently developed highly multiplexed sequencing assays (HMSAs) that rely on high-throughput sequencing can, in principle, meet ...
Bloom, Joshua S. +8 more
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A High-Throughput Method for Illumina RNA-Seq Library Preparation. [PDF]
, 2012 With the introduction of cost effective, rapid, and superior quality next generation sequencing techniques, gene expression analysis has become viable for labs conducting small projects as well as large-scale gene expression analysis experiments. However,
Daniel H Chitwood +8 more
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From meadows to milk to mucosa – adaptation of Streptococcus and Lactococcus species to their nutritional environments [PDF]
, 2011 Lactic acid bacteria (LAB) are indigenous to food-related habitats as well as associated with the mucosal surfaces of animals. The LAB family Streptococcaceae consists of the genera Lactococcus and Streptococcus.
Adab, P. +12 more
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Diagnostic applications of next generation sequencing: working towards quality standards [PDF]
, 2012 Over the past 6 years, next generation sequencing (NGS) has been established as a valuable high-throughput method for research in molecular genetics and has successfully been employed in the identification of rare and common genetic variations. All major
Adey +42 more
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Nature Communications, 2023 MicroRNAs (miRNAs) regulate fundamental biological processes by silencing mRNA targets and are dysregulated in many diseases. Therefore, miRNA replacement or inhibition can be harnessed as potential therapeutics.
Lien D. Nguyen +11 more
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Diverse and widespread contamination evident in the unmapped depths of high throughput sequencing data [PDF]
, 2014 Background: Trace quantities of contaminating DNA are widespread in the laboratory environment, but their presence has received little attention in the context of high throughput sequencing.
Lusk, Richard W
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High-throughput single-сell sequencing in cancer research
Signal Transduction and Targeted Therapy, 2022 With advances in sequencing and instrument technology, bioinformatics analysis is being applied to batches of massive cells at single-cell resolution. High-throughput single-cell sequencing can be utilized for multi-omics characterization of tumor cells,
Qingzhu Jia +4 more
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Deep Sequencing Analysis of RNAs from Citrus Plants Grown in a Citrus Sudden Death-Affected Area Reveals Diverse Known and Putative Novel Viruses. [PDF]
, 2017 Citrus sudden death (CSD) has caused the death of approximately four million orange trees in a very important citrus region in Brazil. Although its etiology is still not completely clear, symptoms and distribution of affected plants indicate a viral ...
Coletta-Filho, Helvecio D +7 more
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High Throughput Sequencing: An Overview of Sequencing Chemistry [PDF]
Indian Journal of Microbiology, 2016 In the present century sequencing is to the DNA science, what gel electrophoresis was to it in the last century. From 1977 to 2016 three generation of the sequencing technologies of various types have been developed. Second and third generation sequencing technologies referred commonly to as next generation sequencing technology, has evolved ...
Sheetal, Ambardar +4 more
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High-throughput sequencing of a South American Amerindian. [PDF]
PLoS ONE, 2013 The emergence of next-generation sequencing technologies allowed access to the vast amounts of information that are contained in the human genome. This information has contributed to the understanding of individual and population-based variability and ...
André M Ribeiro-dos-Santos +11 more
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