Results 101 to 110 of about 109,071 (235)

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source

Antibiofilm activity of a chionodracine‐derived peptide by NMR‐based metabolomics of cell‐free supernatant of Acinetobacter baumannii clinical strains

FEBS Open Bio, EarlyView.
KHS‐Cnd peptide is able to impair biofilm formation and disaggregate mature biofilms in Acinetobacter baumannii clinical isolates. Differences in extracellular metabolites reflect changes in biofilm metabolism due to KHS‐Cnd treatment. Among the differentially represented extracellular metabolites upon KHS‐Cnd treatment, the significantly altered ...
Fernando Porcelli, Enrico Landi, Francesco Maiurano, Irene Paris, Rosanna Papa, Marco Artini, Laura Selan, Stefano Borocci, Francesco Buonocore, Esther Imperlini   +9 more
wiley   +1 more source

Associative and non-associative plasticity in Kenyon cells of the honeybee mushroom body

Frontiers in Systems Neuroscience, 2008
The insect mushroom bodies are higher-order brain centers and critical for odor learning. We investigated experience dependent plasticity of their intrinsic neurons, the Kenyon cells.
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

原料调质对小榨花生油风味及品质的影响Effects of raw material conditioning on flavor and quality of pressed flavor peanut oil

Zhongguo youzhi
摘要：为提升小榨花生油的风味品质，以进口苏丹花生仁为原料，分别采用直接炒籽、炒籽后蒸汽调质、炒籽前蒸汽调质、炒籽前润水调质4种方式对花生仁进行处理，压榨制备小榨花生油，考察了不同调质方式及润水调质后不同放置时间对花生油基本理化指标和风味的影响。结果表明：炒籽前后蒸汽调质所得花生油色泽较直接炒籽明显增加，其中红值（R）增加0.9~1.2，黄值（Y）增加10，而炒籽前润水调质所得花生油色泽明显降低，其中R降低1.4，Y降低10；炒籽前蒸汽调质所得花生油磷含量最低，为256.93 mg/kg ...
王蓓蓓, 刘配莲，董西余，陈文娜，潘亚萍 WANG Beibei, LIU Peilian,DONG Xiyu, CHEN Wenna, PAN Yaping
doaj   +1 more source

Engineering tandem VHHs to target different epitopes to enhance antibody‐dependent cell‐mediated cytotoxicity

FEBS Open Bio, EarlyView.
Tandem VHH targeting distinct EGFR epitopes were engineered into a monovalent bispecific antibody (7D12‐EGA1‐Fc) with more potent ADCC without increasing affinity to EGFR. Structural modeling of 7D12‐EGA1‐Fc showed cross‐linking of separate EGFR domains to enhance CD16a engagement on NK cells.
Yuqiang Xu, Hao Jiang, Limin Chen, Fulai Zhou, Ying Jin, Mark L. Chiu   +5 more
wiley   +1 more source

Infantile Amnesia can be Operationalized as a Psychological Meta Norm in the Development of Memory

Journal of Integrative Neuroscience
This paper offers a syncretic synthesis of the highlights of the scientific knowledge accumulated to date on the mechanisms of infantile amnesia (IA). IA can be conceptualized as a meta-norm of memory development.
Bozhidara Stoencheva, Kristina Stoyanova, Drozdstoy Stoyanov   +2 more
doaj   +1 more source

Evaluation of in vitro toxicity of common phytochemicals included in weight loss supplements using 1H NMR spectroscopy

FEBS Open Bio, EarlyView.
We investigated the toxicity of 12 active compounds commonly found in herbal weight loss supplements (WLS) using human liver and colon cell models. Epigallocatechin‐3‐gallate was the only compound showing significant toxicity. Metabolic profiling revealed protein degradation, disrupted energy and lipid metabolism suggesting that the inclusion of EGCG ...
Emily C. Davies, Garth L. Maker, Ian F. Musgrave, Samantha Lodge   +3 more
wiley   +1 more source

Functional dissection of a neuronal brain circuit mediating higher-order associative learning

Cell Reports
Summary: A central feature characterizing the neural architecture of many species’ brains is their capacity to form associative chains through learning.
El Yazid Rachad, Stephan Hubertus Deimel, Lisa Epple, Yogesh Vasant Gadgil, Anna-Maria Jürgensen, Magdalena Springer, Chen-Han Lin, Martin Paul Nawrot, Suewei Lin, André Fiala   +9 more
doaj   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source