Results 101 to 110 of about 164 (159)

Changes in the Aqueous Solvent do not Impact the Internal Ring‐Flip Dynamic of Fully Buried F52 in Protein GB1

ChemBioChem, EarlyView.
The ring‐flip dynamic in the interior of a protein is independent from changes in the solvent and only depends on the protein itself. Aromatic ring flips are a hallmark of protein dynamics. They are mediated by either transient “breathing” motions in which the protein expands into the solvent or by transient internal rearrangement of void spaces ...
Matthias Dreydoppel, Mikhail Achkinazi, Charlotte Krünholz, Paula L. Jordan, Ulrich Weininger   +4 more
wiley   +1 more source

Exploiting SpyTag/SpyCatcher Technology to Design New Artificial Catalytic Copper Proteins

ChemBioChem, EarlyView.
Designing artificial metal sites in proteins is challenging due to the need to place the metal site in precise positions and to tailor the coordination environment of the metal. A modular approach based on the SpyTag/SpyCatcher technology is used to provide the Spy construct with a Cu2+/ATCUN site.
Silvia Gentili, Francesca Miglioli, Valentina Borghesani, Gloria Spagnoli, Denise Bellotti, Davide Cavazzini, Remo Guerrini, Maurizio Remelli, Giovanni Maestri, Simone Ottonello, Angelo Bolchi, Matteo Tegoni   +11 more
wiley   +1 more source

Covalent Peptide‐Graphene Conjugates for Enhanced Cell Spreading, Osteogenic Differentiation, and Angiogenesis in Bone Defects

ChemBioChem, EarlyView.
The three peptides RGD, DGEA, and KKGHK are covalently attached to the 2D nanomaterial graphene oxide (GO) to create new biomaterials for the treatment of severe bone injuries. These GO modifications improve cell spreading, stem cell differentiation into bone cell types, and formation of blood vessel‐like structures.
Michelle E. Wolf, Yaxuan Liu, Jason D. Orlando, Jingzhi Zhou, Stefanie A. Sydlik   +4 more
wiley   +1 more source

A de novo designed metalloprotein displays variable thermal stability and binding stoichiometry with transition metal ions

ChemBioChem, Accepted Article.
Metal‐binding selectivity in natural proteins is determined by multiple factors such as the protein’s structure, metal concentration within cellular compartments and the presence of metallochaperones. The in vitro selectivity of proteins for transition metal ions is largely governed by the Irving‐Williams series, that states protein‐metal complex ...
Britt Rooijakkers, Gaya Verhagen, Anneloes Cramer-Blok, Ed Zuidinga, Aimee Lynn Boyle   +4 more
wiley   +1 more source

Harnessing Organometallic Au(III) Complexes as Precision Scaffolds for Next‐Generation Therapeutic and Imaging Agents

ChemBioChem, EarlyView.
Cyclometalated gold(III) complexes offer a versatile platform for selective biological interactions, including cysteine arylation, redox modulation, and enzyme inhibition. This review explores their roles in anticancer, antimicrobial, and protein modification strategies, highlighting their ability to disrupt metabolic pathways, modulate aquaporins, and
Sophie R. Thomas, Riccardo Bonsignore
wiley   +1 more source

High‐ and Low‐Protein Pea Genotypes: A Comparative Study of the Physicochemical, Functional, and Quality Properties of Protein Isolates

Cereal Chemistry, EarlyView.
ABSTRACT Background and Objectives This study evaluated the effect of seed protein content on the composition, physicochemical, functional, and quality properties of pea protein isolates recovered through alkaline extraction/isoelectric precipitation.
Cassia Galves, Krishna Kishore Gali, Thomas D. Warkentin, James House, Michael Nickerson   +4 more
wiley   +1 more source

Insights into the Mechanism of a Vanadium Bromoperoxidase from the Marine Macro‐Algae Corallina pilulifera for Biocatalytic Halogenation

ChemCatChem, EarlyView.
A study of crystallographic complexes obtained from vanadium haloperoxidase enzymes can determine key amino acids, leucine, and phenylalanine, that change their structural orientation to allow either chloride or bromide to bind to the enzymes active site, thereby determining halide specificity.
Daniel E. Mitchell, Esther Garcia‐Rodriguez, Michail N. Isupov, Jennifer A. Littlechild   +3 more
wiley   +1 more source

Effect of Trimethylamine‐N‐Oxide (TMAO) and Sorbitol on the Catalytic Activity of Candida boidinii Formate Dehydrogenase

ChemCatChem, EarlyView.
The effects of the osmolytes TMAO and sorbitol on the catalytic activity of Candida boidinii formate dehydrogenase are investigated using computational and experimental studies. The osmolytes exert different effects on the inter‐domain fluctuations, solvation, and active site dynamics of the enzyme as well as on the substrate residence times.
Julio A. Perez‐Erviti, Yasser Almeida‐Hernández, Joel Mieres‐Perez, Nicolas Gajardo‐Parra, Roland Winter, Christoph Held, Gabriele Sadowski, Elsa Sanchez‐Garcia   +7 more
wiley   +1 more source

Luminescence and Electroanalytical Properties of Carbon Quantum Dots in the Context of Immunosensor Design

ChemElectroChem, Volume 12, Issue 6, March 15, 2025.
Two types of carbon quantum dots (CQD‐COOH and CQD‐NH2) are used to modify screen printed carbon electrodes, for detection of Cardiac Troponin I (cTnI). These modified sensors offer promising results for the determination of cTnI in the picomolar range, when, using an immunosensor protocol.
Yemataw Addis Alemu, Clementine J Louw, Francesco Paolucci, Giovanni Valenti, Priscilla G L Baker   +4 more
wiley   +1 more source

What Factors Determine the Brevione B Desaturation Mechanism in the Nonheme Iron Dioxygenase BrvJ?

Chemistry – A European Journal, Volume 31, Issue 16, March 17, 2025.
Density functional theory calculations on the nonheme iron desaturase BrvJ show that desaturation happens through two consecutive hydrogen atom abstraction barriers, while the OH rebound barrier is stereochemically hindered. We also tested a second reaction cycle and show that product is not released but can undergo a second cycle with small barriers ...
Jingyu Cao, Jingyuan Lu, Yuanxin Cao, Sam P. de Visser   +3 more
wiley   +1 more source