New Concepts in Therapeutic Manipulation of HIV-1 Transcription and Latency: Latency Reversal versus Latency Prevention [PDF]
Viruses, 2023 Antiretroviral therapy (ART) has dramatically improved the prognosis for people living with HIV-1, but a cure remains elusive. The largest barrier to a cure is the presence of a long-lived latent reservoir that persists within a heterogenous mix of cell ...
Catherine A. Lewis +2 more
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Latency reversal and viral clearance to cure HIV-1 [PDF]
Science, 2016 Next steps toward curing HIV-1 Since the discovery of HIV-1 more than 30 years ago, prevention and treatment strategies have dominated the research agenda. More recently, however, scientists are also focusing their efforts toward finding a cure. Margolis et al.
David M, Margolis +3 more
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Mechanism by Which PF-3758309, a Pan Isoform Inhibitor of p21-Activated Kinases, Blocks Reactivation of HIV-1 Latency [PDF]
Biomolecules, 2023 The “block and lock” strategy is one approach that might elicit a sterilizing cure for HIV-1 infection. The “block” refers to a compound’s ability to inhibit latent HIV-1 proviral transcription, while the “lock” refers to its capacity to induce permanent
Benni Vargas +3 more
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A Two-Color Haploid Genetic Screen Identifies Novel Host Factors Involved in HIV-1 Latency [PDF]
mBio, 2021 To identify novel host factors as putative targets to reverse HIV-1 latency, we performed an insertional mutagenesis genetic screen in a latent HIV-1 infected pseudohaploid KBM7 cell line (Hap-Lat).
Michael Röling +13 more
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A CRISPR Screen of HIV Dependency Factors Reveals That CCNT1 Is Non-Essential in T Cells but Required for HIV-1 Reactivation from Latency [PDF]
Viruses, 2023 We sought to explore the hypothesis that host factors required for HIV-1 replication also play a role in latency reversal. Using a CRISPR gene library of putative HIV dependency factors, we performed a screen to identify genes required for latency ...
Terry L. Hafer +4 more
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Inconsistent Reversal of HIV-1 Latency Ex Vivo by Antigens of HIV-1, CMV, and Other Infectious Agents [PDF]
Retrovirology, 2020 Abstract BackgroundA reservoir of replication-competent but latent virus is the main obstacle to a cure for HIV-infection. Much of this reservoir resides in memory CD4 T cells. We hypothesized that these cells can be reactivated with antigens from HIV and other common pathogens to reverse latency.
Thomas Vollbrecht +6 more
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Sensing of HIV-1 by TLR8 activates human T cells and reverses latency [PDF]
Nature Communications, 2020 Manipulation of Toll-like receptors (TLRs) affects HIV-1 infection and latency reversal. Here, the authors show that HIV-1 is endocytosed and recognized by TLR8 in human primary CD4+T cells and that TLR8 stimulation induces an inflammatory response that ...
Hany Zekaria Meås +11 more
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Trade-off between synergy and efficacy in combinations of HIV-1 latency-reversing agents. [PDF]
PLoS Computational Biology, 2018 Eradicating HIV-1 infection is difficult because of the reservoir of latently infected cells that gets established soon after infection, remains hidden from antiretroviral drugs and host immune responses, and retains the capacity to reignite infection ...
Vipul Gupta, Narendra M Dixit
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HibeRNAtion: HIV-1 RNA Metabolism and Viral Latency [PDF]
Frontiers in Cellular and Infection Microbiology, 2022 HIV-1 infection remains non-curative due to the latent reservoir, primarily a small pool of resting memory CD4+ T cells bearing replication-competent provirus.
Raquel Crespo +4 more
doaj +3 more sources
Epitranscriptomic m6A modifications during reactivation of HIV-1 latency in CD4+ T cells [PDF]
mBioDespite effective antiretroviral therapy reducing HIV-1 viral loads to undetectable levels, the presence of latently infected CD4+ T cells poses a major barrier to HIV-1 cure.
Tarun Mishra +5 more
doaj +2 more sources