Results 171 to 180 of about 118,712 (218)

Colorimetric reverse transcriptase assay for HIV-1

Journal of Virological Methods, 1993
A colorimetric assay for reverse transcriptase (RT) of the human immunodeficiency virus type 1 (HIV-1) was developed using a double labelled (biotin and digoxigenin) deoxyuridine triphosphate mixture instead of tritiated thymidine triphosphate. After the RT reaction, the newly polymerized strand from oligodeoxythymidylic acid (oligo-dT) contained both ...
K, Suzuki, B P, Craddock, T, Kano, R T, Steigbigel   +3 more
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Targeting HIV reverse transcriptase in novel ways

Nature Medicine, 1995
The promising description of a potential basis for gene therapy in treating HIV infection does not mean that traditional approaches should be abandoned (pages 667–673).
M A, Wainberg, Z, Gu
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Novel HIV-1 reverse transcriptase inhibitors

Virus Research, 2008
HIV-1 reverse transcriptase (RT) was the first viral enzyme to be targeted by anti-HIV drugs. Despite 20 years of experience with RT inhibitors, new ways to inhibit this target and address viral resistance continue to emerge. In both licensed RT inhibitor classes, nucleosides (NRTIs) and non-nucleosides (NNRTIs), compounds with better resistance ...
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Chemiluminescent Enzyme-Linked Immunoassay for Reverse Transcriptase, Illustrated by Detection of HIV Reverse Transcriptase

Analytical Biochemistry, 1993
A chemiluminescent assay for reverse transcriptase (RT) of the human immunodeficiency virus 1 was developed using biotin-labeled oligodeoxythymidylic acid (biotin oligo-dT) and digoxigenin-deoxyuridine triphosphate instead of tritiated thymidine triphosphate. After the RT reaction, the newly polymerized strand from biotin oligo-dT contained digoxigenin
K, Suzuki, B P, Craddock, T, Kano, R T, Steigbigel   +3 more
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HIV-1 Reverse Transcriptase Resistance to Nonnucleoside Inhibitors

Biochemistry, 1996
The parameters governing the polymerization mechanism of reverse transcriptase containing the tyrosine to cysteine mutation at position 181 (Y181C) were determined using pre-steady-state techniques. The pathway for single nucleotide incorporation catalyzed by Y181C is similar to that determined for wild-type RT where a rate-limiting conformational ...
R A, Spence, K S, Anderson, K A, Johnson
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Coumarins as Inhibitors of HIV Reverse Transcriptase

Current HIV Research, 2006
Acquired immunodeficiency syndrome (AIDS), a degenerative disease of the immune and central nervous systems, is an enormous world-wide health threat. No cure has been found, and research is aimed at developing chemotherapy against the causative agent, human immunodeficiency virus (HIV).
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HIV Inhibitors Targeted at the Reverse Transcriptase

AIDS Research and Human Retroviruses, 1992
HIV inhibitors targeted at the virus-associated reverse transcriptase (RT) can be divided into two groups, depending on whether they are targeted at the substrate or nonsubstrate binding site. To the first group belong the 2',3'-dideoxynucleosides (i.e., DDC, DDI), 3'-azido-2',3'-dideoxynucleosides (i.e., AZT), 3'-fluoro-2',3'-dideoxynucleosides (i.e.,
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Nontemplated Nucleotide Addition by HIV-1 Reverse Transcriptase

Biochemistry, 2002
We studied the kinetics of nontemplated nucleotide addition by the reverse transcriptase (RT) of human immunodeficiency virus type 1 (HIV-1) using model substrates derived from the 3' end of HIV-1 minus-strand strong-stop DNA. The addition of a nontemplated nucleotide was highly dependent on the nature of the base (fastest addition with dATP), type of ...
Marie-Pierre, Golinelli, Stephen H, Hughes   +1 more
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The Accuracy of Reverse Transcriptase from HIV-1

Science, 1988
A study was conducted to determine the fidelity of DNA synthesis catalyzed in vitro by the reverse transcriptase from a human immunodeficiency virus type 1 (HIV-1). Like other retroviral reverse transcriptases, the HIV-1 enzyme does not correct errors by exonucleolytic proofreading.
J D, Roberts, K, Bebenek, T A, Kunkel
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An HIV Reverse Transcriptase-Selective Nucleoside Chain Terminator

Journal of the American Chemical Society, 2002
The synthesis of a 2',3'-dideoxynucleoside cytidine analogue, but one that lacks the O2-carbonyl, is described from 2-aminopyridine in an overall yield of 60%. The synthesis of the 2-pyridone C-nucleoside relies upon the use of a Heck-type coupling between an appropriately protected sugar glycal and the 5-iodo derivative of 2-aminopyridone.
Andrew W, Fraley, Dongli, Chen, Kenneth, Johnson, Larry W, McLaughlin   +3 more
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