Results 41 to 50 of about 50,120 (254)

Dataset from proteomic analysis of human liver, lung, kidney and intestine microsomes

Data in Brief, 2018
We provide detailed datasets from our analysis of proteins that are identified in human liver, lung, kidney and intestine microsomes by MS-based proteomics.
Wei Song, Longjiang Yu, Zhihong Peng
doaj   +1 more source

Interspecies Variation in NCMN-O-Demethylation in Liver Microsomes from Various Species

Molecules, 2019
NCMN (N-(3-carboxy propyl)-4-methoxy-1,8-naphthalimide), a newly developed ratiometric two-photon fluorescent probe for human Cytochrome P450 1A (CYP1A), shows the best combination of specificity and reactivity for real-time detection of the enzymatic ...
Ziru Dai, Guibo Sun, Jiada Yang, Jie Hou, Ping Zhou, Weijie Xie, Guangbo Ge, Xiaobo Sun, Ling Yang   +8 more
doaj   +1 more source

Characterisation of theophylline metabolism by human liver microsomes [PDF]

Biochemical Pharmacology, 1987
1. A radiometric high performance liquid chromatographic method is described for the assay of theophylline metabolism in vitro by the microsomal fraction of human liver. 2. Formation of the three metabolites of theophylline (3‐methylxanthine, 1‐methylxanthine and 1,3‐ dimethyluric acid) were linear with protein concentrations to 4 mg ml‐1 and with ...
R A, Robson, A P, Matthews, J O, Miners, M E, McManus, U A, Meyer, P M, Hall, D J, Birkett   +6 more
openaire   +2 more sources

In Vitro Phase I Metabolism of CRV431, a Novel Oral Drug Candidate for Chronic Hepatitis B

Pharmaceutics, 2017
The cytochrome P450-mediated Phase I in vitro metabolism of CRV431 was studied using selective chemical inhibition and recombinant human enzymes. Additionally, the metabolic profile of CRV431 in human, rat, and monkey liver microsomes was investigated ...
Daniel J. Trepanier, Daren R. Ure, Robert T. Foster   +2 more
doaj   +1 more source

Simultaneous determination of 9 CYP450 enzymes in human liver microsomes using LC-MS/MS

Di-san junyi daxue xuebao, 2021
Objective To develop a liquid chromatography with tandem mass spectrometry (LC-MS/MS) to simultaneously detect the activities of 9 CYP450 enzymes in human liver microsomes in order to anticipate the possibility of drug-drug interactions.
SHI Zhongbo, ZHAO Dezhang, JIANG Junhao, XIE Baogang, LI Yan, YU Chao   +5 more
doaj   +1 more source

Stereoselective Biotransformation: Transfer of Learning to Advance Drug Metabolism and Biocatalysis

Angewandte Chemie, EarlyView.
Understanding stereoselective biotransformations has implications for predicting drug disposition and response and may also inspire novel biocatalytic and biomimetic strategies to address challenges in metabolite and API synthesis. ABSTRACT Chirality is an important determinant of drug action, as enantiomers can exhibit markedly different ...
Grace A. Okunlola, Godwin A. Aleku
wiley   +2 more sources

UPLC-MS/MS analysis of ochratoxin A metabolites produced by Caco-2 and HepG2 cells in a co-culture system [PDF]

, 2017
Ochatoxin A (OTA) is one of the most important mycotoxins based on its toxicity. The oral route is the main gateway of entry of OTA into the human body, and specialized epithelial cells constitute the first barrier.
González Arias, Cyndia A., Marín Sillué, Sònia, Ramos Girona, Antonio J., Rojas-García, A. E., Sanchís Almenar, Vicente   +4 more
core   +2 more sources

Stereoselective glucuronidation of formoterol by human liver microsomes [PDF]

British Journal of Clinical Pharmacology, 2000
Aims Formoterol is a β2‐adrenoceptor agonist marketed as a racemic mixture of the active (R; R)‐ and inactive (S; S)‐enantiomers (rac‐formoterol). The drug produces prolonged bronchodilation by inhalation but there is significant interpatient variability in duration of effect.
M, Zhang, J P, Fawcett, J M, Kennedy, J P, Shaw   +3 more
openaire   +2 more sources

Involvement of cytochrome P450 2E1 in the (ω–1)-hydroxylation of oleic acid in human and rat liver microsomes

Journal of Lipid Research, 1998
In vitro techniques have been used to investigate the nature of microsomal cytochrome P450 involved in the metabolism of oleic acid, a physiological monounsaturated fatty acid. Like lauric acid, which is cur rently used as a model substrate of fatty acid
Fadi Adas, François Berthou, Daniel Picart, Patrick Lozac'h, Françoise Beaugé, Yolande Amet   +5 more
doaj   +1 more source

Qualification of Human Liver Microsomes for Antibacterial Activity Screening of Drug Metabolites

Applied Microbiology, 2023
Microsomes are commonly used to perform in vitro drug metabolism, predominantly to form phase I drug metabolites. Pooled microsomes from multiple donors can contain microorganisms from underlying microbial diseases.
Navid Jubaer
doaj   +1 more source