Antithymocyte Globulins Bind to Human Umbilical Vein Endothelial Cells
Transplantation Proceedings, 2009Polyclonal antithymocyte globulins (ATGs) are immunosuppressive agents used for the treatment of organ rejection after transplantation. ATGs induce complement-mediated cell death of T lymphocytes and may decrease leukocyte adhesion. However, little is known about their effects on endothelial cells (ECs).
A, Beiras-Fernandez +6 more
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Enterovirus infection and activation of human umbilical vein endothelial cells
Journal of Medical Virology, 2003AbstractGastrointestinal tract associated lymphoid tissue is considered to be the main replication site for enteroviruses. In order to invade tissues to reach pancreatic islets, cardiac muscles, and other secondary replication sites, the virus has to survive circulation in the blood and find a way to get through endothelial cells. In the present study,
Salla, Saijets +4 more
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Apoptosis of human umbilical vein endothelial cells induced by artesunate
Vascular Pharmacology, 2004Artesunate (ART), a semi-synthetic derivative of artemisinin isolated from the traditional Chinese herb Artemisia annua, is an effective novel antimalarial drug. The present study investigated the apoptotic activity of artesunate in cultured human umbilical vein endothelial cell (HUVEC) by means of nuclear staining, DNA agarose gel electrophoresis, and
Guo-Dong, Wu, Hui-Jun, Zhou, Xiu-Hua, Wu
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Visualization of human umbilical vein endothelial cells by acoustic microscopy
Ultrasonics, 2000The morphology and acoustic properties of the human umbilical vein endothelial cells (HUVECs) were evaluated using a scanning acoustic microscope system. HUVECs were cultured for 4 days and exposed to the endotoxin for 4 h. The frequency of the scanning acoustic microscope was variable between 100 and 210 MHz.
Y, Saijo +4 more
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Homocysteine uptake by human umbilical vein endothelial cells in culture
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1990The characteristics of the uptake of L-homocysteine by cultures of human umbilical vein endothelial cells have been examined. Uptake occurred by Na(+)-dependent and Na(+)-independent systems, but was essentially independent of the pH of the uptake medium.
M J, Ewadh, N, Tudball, F A, Rose
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Fibrinolytic Proteins in Apoptotic Human Umbilical Vein Endothelial Cells
Thrombosis Research, 1998Endothelial cells express fibrinolytic proteins including: urokinase (u-PA) and tissue type (t-PA) plasminogen activators, type-1 (PAI-1) and 2 (PAI-2) plasminogen activator inhibitors, and u-PA receptor (u-PAR). Apoptotic endothelial cells detach, potentially forming both local and circulating microthrombi in vivo.
H, Zoellner +6 more
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Antiapoptotic effect of ouabain on human umbilical vein endothelial cells
Biochemical and Biophysical Research Communications, 2004The present study investigates the effect of ouabain on caspase-3 activation in human umbilical vein endothelial cells (HUVEC). Ouabain (EC(50) 20 nM) reduced caspase-3 activity in HUVEC treated for 24h in a medium deprived of fibroblast growth factor (FGF).
TREVISI, LUCIA +4 more
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Isolation and Culture of Human Umbilical Vein Endothelial Cells (HUVECs)
2023This protocol describes a simple and an economical method for isolation of endothelial cells from human umbilical vein. Umbilical cord is easily available postpartum following informed consent, and the method for its collection is noninvasive with few ethical concerns.
Shivam, Chandel +3 more
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A Comparison of Substrates for Human Umbilical Vein Endothelial Cell Culture
Biotechnic & Histochemistry, 1992We have studied culture conditions which facilitate the growth of stable, non-proliferating, human umbilical vein endothelial cell (HUVEC) monolayers. Gelatin and fibronectin coatings, with or without glutaraldehyde cross-linking, on both plastic and glass were investigated for initial attachment of HUVEC and growth characteristics. The presence during
E F, Smeets +4 more
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A protocol for isolation and culture of human umbilical vein endothelial cells
Nature Protocols, 2007We describe a protocol for easy isolation and culture of human umbilical vein endothelial cells (HUVECs) to supply every researcher with a method that can be applied in cell biology laboratories with minimum equipment. Endothelial cells (ECs) are isolated from umbilical vein vascular wall by a collagenase treatment, then seeded on fibronectin-coated ...
Bruno, Baudin +3 more
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