Results 41 to 50 of about 8,263 (198)

SynNotch Receptors for Visualizing Immunoreceptor Force Transmission and Downstream Signaling In Vivo

Advanced Science, EarlyView.
A mechanically activated SynNotch reporter platform enables detection of force transmission through receptors as they mediate immune cell interactions. Targeting CD40 on B cells and TCR on T cells records force‐induced reporter activation across 2D coculture, 3D organoid, and implanted in mice, which also reveal force‐amplification of immune receptor ...
Menglan Li, Jintian Lyu, Kaitao Li, Ameya Dravid, Deepali Balasubramani, Amir H. K. Ashkezari, Hyun‐Kyu Choi, Gabriel A. Kwong, Ankur Singh, Cheng Zhu   +9 more
wiley   +1 more source

Toward a molecular understanding of adaptive immunity:A chronology, Part II

Frontiers in Immunology, 2012
By 1980 it was obvious that to more fully understand adaptive immunity, one needed to somehow reduce the tremendous complexity of antigen recognition by T cell populations. Thus, there were two developments that resulted in a paradigm shift in immunology,
Kendall A Smith
doaj   +1 more source

GroEL Chaperonin-Based Assay for Early Diagnosis of Scrub Typhus

Diagnostics, 2022
A point-of-care diagnostic for early and rapid diagnosis of scrub typhus caused by Orientia tsutsugamushi is required for prompt and proper treatment of patients presenting with undifferentiated febrile illnesses.
Nitaya Indrawattana, Pisinee Aiumurai, Nawannaporn Sae-lim, Watee Seesuay, Onrapak Reamtong, Manas Chongsa-nguan, Wanpen Chaicumpa, Nitat Sookrung   +7 more
doaj   +1 more source

A Synthetic Platform for Antibody Junctional Diversification Beyond Natural Constraints

Advanced Science, EarlyView.
ARESEC is a synthetic platform that reconstructs functional V(D)J recombination in HEK293T cells. By introducing RSS elements and co‐expressing RAG1/2 and TdT across all three antibody CDRs, it enables programmable junctional diversification and direct generation of high‐affinity variants targeting PD‐1/PD‐L1, overcoming the evolutionary constraints of
Wang Liu, Jun Yang, Yuhang Zhang, Xiaoli Xue   +3 more
wiley   +1 more source

Characterization of positive hybridoma clone.

, 2019
(A) The isotype result of monoclonal antibody from 1E6A4 hybridoma cells. The experiment of antibody isotyping was conducted with isotyping kit, and the isotype of 1E6A4 is IgG2a. (B) Chromosome analysis of 1E6A4 hybridoma cell (10×100).
Kunzhi Jia (323418), Yaju Liu (6672512), Qinghai Yang (6672515), Xiangzhu Kong (2609803), Huiling Chen (9525), Shihua Wang (219946), Danping Zhang (4491217), Yanghai Wang (6672509), Chengjie Xie (6672518)   +8 more
core   +1 more source

ZBED6 Knockout Promotes Skeletal Muscle Development in Bama Pigs by Relieving Transcriptional Repression of CDKN1A

Animal Research and One Health, EarlyView.
Integrated transcriptomic and functional analyses identify CDKN1A as a direct target of ZBED6 in Bama pigs. ZBED6 knockout relieves transcriptional repression of CDKN1A, thereby promoting myogenic differentiation and skeletal muscle development.
Yitian Ma, Xiu Lin, Pengtao Yuan, Yabin Pu, Yuehui Ma, Zixiao Jiang, Lin Jiang, Chongyang Li, Dandan Wang   +8 more
wiley   +1 more source

Immune Complexes Shape the Pathogenicity of Autoantibodies in Systemic Autoimmune Diseases: Insights From an FcγRIIIA‐Reporter Assay

Arthritis &Rheumatology, EarlyView.
Objective Immune complexes (ICs), formed by autoantigen and autoantibody, play a pathogenic role in systemic autoimmune diseases through stimulation of Fcγ receptors (FcγR). However, studies investigating bioactivity of circulating ICs across various diseases remain limited.
Koji Suzuki, Masaru Takeshita, Yuki Imai, Hiroyuki Fukui, Kazunori Furuhashi, Kotaro Matsumoto, Jun Kikuchi, Keiko Yoshimoto, Yuko Kaneko   +8 more
wiley   +1 more source

Targeted photodynamic therapy of cancer using a novel gallium (III) tris (ethoxycarbonyl) corrole conjugated‐mAb directed against cancer/testis antigens 83

Cancer Medicine, 2018
Photodynamic therapy (PDT) is a noninvasive, highly selective approach to the treatment of tumors. However, its therapeutic effect is limited by long‐lasting skin phototoxicity.
Ziyu Ye, Yanfang Liang, Yan Ma, Bihua Lin, Longbin Cao, Bin Wang, Zhao Zhang, Haibo Yu, Jixia Li, Mingyuan Huang, Keyuan Zhou, Qunzhou Zhang, Xinguang Liu, Jincheng Zeng   +13 more
doaj   +1 more source

Comparative Analysis of Bioreactor Design and Scale‐Up for Cultivated Meat Using Monte Carlo‐Based Timescale Modeling

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Cultivated meat offers a sustainable alternative to conventional livestock production but faces significant scale‐up challenges, particularly in bioreactor design for mammalian cell suspension cultures. This study presents a comparative analysis of stirred tank reactors (STR), bubble columns (BC), and airlift reactors (ALR) to assess their ...
Pieter Jan Theodoor Brorens, Marcel Ottens, Cees Haringa   +2 more
wiley   +1 more source

Immune response to progesterone immobilized on Cu2+- induced amphifilic polyelectrolyte-protein complex: Antigen specificity and affinity of hybridoma clones

, 2001
Cu2+-mediated complex formation between copolymers of acrylic acid with N-isopropyl-acyrlamide (CP1) and negatively charged covalent conjugate of bovine serum albumin with progesterone (BSA.P) was studied in neutral water in the presence of Cu2+.

core   +1 more source