Results 21 to 30 of about 8,263 (198)
A New Workflow to Generate Monoclonal Antibodies against Microorganisms
Applied Sciences, 2021 Monoclonal antibodies are used worldwide as highly potent and efficient detection reagents for research and diagnostic applications. Nevertheless, the specific targeting of complex antigens such as whole microorganisms remains a challenge.
Markus Göthel +5 more
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Applied Sciences, 2021 One of the most important limitations of mammalian cells-based bioprocesses, and particularly hybridoma cell lines, is the accelerated metabolism related to glucose and glutamine consumption. The high uptake rates of glucose and glutamine (i.e., the main
Iván Martínez-Monge +7 more
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Towards Identifying Protective B-Cell Epitopes: The PspA Story
Frontiers in Microbiology, 2017 Pneumococcal surface protein A (PspA) is one of the most abundant cell surface protein of Streptococcus pneumoniae (S. pneumoniae). PspA variants are structurally and serologically diverse and help evade complement-mediated phagocytosis of S. pneumoniae,
Naeem Khan, Arif T. Jan
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Tumor Biology, 2017 The 4-1BB is a surface glycoprotein that pertains to the tumor necrosis factor–receptor family. There is compelling evidence suggesting important roles for 4-1BB in the immune response, including cell activation and proliferation and also cytokine ...
Salman Bagheri +8 more
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, 1996 Complex formation between synthetic polyelectrolytes (PE) [poly-4-vinyl-N-ethyl (cetyl), pyridine bromides-PVP(R(2),R(16))], bovine serum albumin (BSA), or 17 beta-estradiol-BSA conjugate (BSA.E) was studied in neutral water, Weakly water-soluble ...
Basalp, A +5 more
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Assessing two strategies for production of murine ascites with anti-SARS-CoV-2 monoclonal antibodies
Cluj Veterinary Journal, 2021 Studies were conducted to improve the production of murine ascites with monoclonal antibodies that recognize SARS-CoV-2 proteins. BALB/c mice were primed with 0.5 mL of mineral oil into the abdominal cavity.
Joel Javier Pérez-Paz +11 more
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Immunochemical evidence that human apoB differs when expressed in rodent versus human cells
Journal of Lipid Research, 2003 LDL from human apolipoprotein B-100 (apoB-100) transgenic (HuBTg+/+) mice contains more triglyceride than LDL from normolipidemic subjects. To obtain novel monoclonal antibody (MAb) probes of apoB conformation, we generated hybridomas from HuBTg+/+ that ...
Xingyu Wang +9 more
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Frontiers in Endocrinology, 2020 In vertebrate germ cell differentiation, gonadal somatic cells and germ cells are closely related. By analyzing this relationship, it has recently been reported in mammals that primordial germ cells (PGCs), induced from pluripotent stem cells and ...
Toshiaki Kawabe +9 more
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Monitoring lysosomal fusion in electrofused hybridoma cells [PDF]
, 2008 Dendritic and tumor cells are fused to produce hybridoma cells, which are considered to be used as cellular vaccines to treat cancer. Previous strategies for hybridoma cell production were based on the quantification of the electrofusion yield by ...
Zorec, Robert +5 more
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DNA immunization as a technology platform for monoclonal antibody induction
Emerging Microbes and Infections, 2016 To combat the threat of many emerging infectious diseases, DNA immunization offers a unique and powerful approach to the production of high-quality monoclonal antibodies (mAbs) against various pathogens.
Shuying Liu, Shixia Wang, Shan Lu
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