Results 41 to 50 of about 8,191,811 (190)
BMC Biotechnology, 2010 Background The generation of monoclonal antibodies specific for protein antigens usually depends on purified recombinant protein for both immunisation and hybridoma screening.
Matile Hugues +3 more
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Large Scale Generation and Characterization of Anti-Human CD34 Monoclonal Antibody in Ascetic Fluid of Balb/c Mice [PDF]
Advanced Pharmaceutical Bulletin, 2013 Purpose: Monoclonal antibodies or specific antibodies are now an essential tool of biomedical research and are of great commercial and medical value. The purpose of this study was to produce large scale of monoclonal antibody against CD34 in order to ...
Koushan Sineh sepehr +6 more
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Advanced Science, EarlyView.A mechanically activated SynNotch reporter platform enables detection of force transmission through receptors as they mediate immune cell interactions. Targeting CD40 on B cells and TCR on T cells records force‐induced reporter activation across 2D coculture, 3D organoid, and implanted in mice, which also reveal force‐amplification of immune receptor ...
Menglan Li +9 more
wiley +1 more source
A Synthetic Platform for Antibody Junctional Diversification Beyond Natural Constraints
Advanced Science, EarlyView.ARESEC is a synthetic platform that reconstructs functional V(D)J recombination in HEK293T cells. By introducing RSS elements and co‐expressing RAG1/2 and TdT across all three antibody CDRs, it enables programmable junctional diversification and direct generation of high‐affinity variants targeting PD‐1/PD‐L1, overcoming the evolutionary constraints of
Wang Liu +3 more
wiley +1 more source
, 2010 The functions of the precursor H antigen for ABO blood group antigens are still not fully understood, particularly in cancer cells. In this study, we used hybridoma technology and NSY human colon cancer cells as an immunogen to generate a monoclonal ...
Gildersleeve, Jeffrey C. +7 more
core +1 more source
Toxins, 2017 Domoic acid (DA) is a potent toxin, marine biotoxin, and primarily produced by Pseudo-nitzschia. The DA hapten was coupled with bovine serum albumin (BSA), and ovalbumin (OVA) as carrier proteins.
Abdullah F. U. H. Saeed +3 more
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Region Met225 to Ile412 of Bacillus cereus Hemolysin II Is Capable to Agglutinate Red Blood Cells
Molecules, 2023 Hemolysin II (HlyII) is one of the virulence factors of the opportunistic bacterium Bacillus cereus belonging to the group of β-pore-forming toxins. This work created a genetic construct encoding a large C-terminal fragment of the toxin (HlyIILCTD, M225 ...
Alexey S. Nagel +8 more
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Animal Research and One Health, EarlyView.Integrated transcriptomic and functional analyses identify CDKN1A as a direct target of ZBED6 in Bama pigs. ZBED6 knockout relieves transcriptional repression of CDKN1A, thereby promoting myogenic differentiation and skeletal muscle development.
Yitian Ma +8 more
wiley +1 more source
Monoclonal Antibodies: from Development to Clinical Application
Клиническая онкогематология, 2016 The development of monoclonal antibodies (MABs) resulted in revolutionary achievements in diagnosing and treating of oncohematological disorders. The review dwells on the history of the development and improved technologies for production of monoclonal ...
Yurii Ivanovich Budchanov
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Hybridoma technology for the production of monoclonal antibodies
Demiroglu Science University Florence Nightingale Journal of Medicine, 2022 Given the size and scope of the global pharmaceutical market, monoclonal antibodies (mAbs) have a promising future in a broad range of areas such as autoimmune diseases, infectious diseases, and cancer therapies. A decisive medical treatment is achievable since mAbs only identify their target antigens and not additional irrelevant ...
Ahmet Sait +3 more
openaire +1 more source