Results 21 to 30 of about 8,191,811 (190)

Design of a bubble-swarm bioreactor for animal cell culture [PDF]

, 1994
Gudermann F, Lütkemeyer D, Lehmann J. Design of a bubble-swarm bioreactor for animal cell culture. Cytotechnology. 1994;15(1-3):301-309.A stationary bubble-swarm has been used to aerate a mammalian cell culture bioreactor with an extremely low gas flow ...
Lehmann, Jürgen, Gudermann, F., Lütkemeyer, Dirk   +2 more
core   +1 more source

Monomethyl auristatin E Exhibits Potent Cytotoxic Activity against Human Cancer Cell Lines SKBR3 and HEK293

Novelty in Biomedicine, 2017
Background: Monomethyl auristatin E (MMAE) is a synthetic analog of dolastatin 10, a compound originally isolated from the marine mollusk. MMAE, as a highly potent microtubule inhibitor, exerts its potent cytotoxic effect by inhibiting microtubule ...
Meghdad Abdollahpour-Alitappeh, Sepand Razavi-vakhshourpour, Majid Lotfinia, Saeed Jahandideh, Hamid Najminejad, Saeed Balalaie, Reza Moazzami, Elnaz Shams, Mahdi Habibi-Anbouhi, Mohsen Abolhassani   +9 more
doaj   +1 more source

Monomethyl Auristatin E, a Potent Cytotoxic Payload for Development of Antibody-Drug Conjugates against Breast Cancer

Novelty in Biomedicine, 2017
Background: Breast cancer is a heterogeneous disease characterized by differential responses to targeted and chemotherapeutic agents. Antibody-drug conjugates are one of the promising strategies for the treatment of breast cancer. Monomethyl auristatin E
Meghdad Abdollahpour-alitappeh, Amir Amanzadeh, Fatemeh Heidarnejad, Mahdi Habibi-Anbouhi, Majid Lotfinia, Sepand Razavi-vakhshourpour, Saeed Jahandideh, Hamid Najminejad, Saeed Balalaie, Mohsen Abolhassani   +9 more
doaj   +1 more source

Antibody Screening by Microarray Technology—Direct Identification of Selective High-Affinity Clones

Antibodies, 2020
The primary screening of hybridoma cells is a time-critical and laborious step during the development of monoclonal antibodies. Often, critical errors occur in this phase, which supports the notion that the generation of monoclonal antibodies with ...
Martin Paul, Michael G. Weller
doaj   +1 more source

Chimerization of antibodies by isolation of rearranged genomic variable regions by the polymerase chain reaction [PDF]

, 1991
We describe a new method for amplification, by polymerase chain reaction (PCR), of rearranged segments encoding the variable part of light and heavy chains of an antibody (Ab) from the chromosomal DNA of hybridoma cells for the chimerization ofAbs.
Schwirzke, Marina, Kaluzab, Brigitte, Weissenhorn, Winfried, Weiss, Elisabeth H., Weidle, Ulrich H.   +4 more
core   +1 more source

Hybridoma Technology

, 2022
Hybridoma technology combines the unique qualities of antibody-producing B cells with immortalized myeloma cells, creating hybrid cells capable of endless proliferation while retaining the ability to produce specific antibodies. The process begins with the immunization of a host animal, typically a mouse, followed by the fusion of its spleen cells ...
openaire   +2 more sources

In vivo Phage Display: A promising selection strategy for the improvement of antibody targeting and drug delivery properties

Frontiers in Microbiology, 2022
The discovery of hybridoma technology, described by Kohler and Milstein in 1975, and the resulting ability to generate monoclonal antibodies (mAbs) initiated a new era in antibody research and clinical development.
Ana S. André, Ana S. André, Isa Moutinho, Isa Moutinho, Joana N. R. Dias, Joana N. R. Dias, Frederico Aires-da-Silva, Frederico Aires-da-Silva   +7 more
doaj   +1 more source

The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator [PDF]

, 1994
Heidemann R, Riese U, Lütkemeyer D, Büntemeyer H, Lehmann J. The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator. Cytotechnology.
Heidemann, R., Riese, U., Lehmann, Jürgen, Büntemeyer, Heino, Lütkemeyer, Dirk   +4 more
core   +1 more source

Development of ELISA and Lateral Flow Immunoassays for Ochratoxins (OTA and OTB) Detection Based on Monoclonal Antibody

Frontiers in Cellular and Infection Microbiology, 2020
Ochratoxins were important secondary metabolites secreted by fungi, and OTA and OTB are mainly significant mycotoxin, having toxic effects on humans and animals.
Mohamed Hassan Fadlalla, Mohamed Hassan Fadlalla, Sumei Ling, Sumei Ling, Sumei Ling, Rongzhi Wang, Rongzhi Wang, Rongzhi Wang, Xiulan Li, Xiulan Li, Jun Yuan, Jun Yuan, Shiwei Xiao, Shiwei Xiao, Ke Wang, Ke Wang, Shuqin Tang, Shuqin Tang, Hoyda Elsir, Hoyda Elsir, Shihua Wang, Shihua Wang, Shihua Wang   +22 more
doaj   +1 more source

A Rapid Method to Characterize Mouse IgG Antibodies and Isolate Native Antigen Binding IgG B Cell Hybridomas.

PLoS ONE, 2015
B cell hybridomas are an important source of monoclonal antibodies. In this paper, we developed a high-throughput method to characterize mouse IgG antibodies using surface plasmon resonance technology.
Haolin Liu, Janice White, Frances Crawford, Niyun Jin, Xiangwu Ju, Kangtai Liu, Chengyu Jiang, Philippa Marrack, Gongyi Zhang, John W Kappler   +9 more
doaj   +1 more source