Results 11 to 20 of about 8,191,811 (190)

Polymeric Approach to Adjuvant System in Antibody Production against Leishmaniasis Based on Hybridoma Technology [PDF]

Iranian Journal of Parasitology, 2022
Background: Leishmaniasis is a zoonotic disease, which is one of the serious public health problems in the world. Nowadays, antibody production using hybridoma technology may be a correct approach in terms of sensitivity in the diagnosis of diseases ...
Asli Pinar Zorba Yildiz, Gulnaz Yildirim Koken, Emrah Sefik Abamor, Melahat Bagirova, Ozlem Ayse Tosyali, Tanil Kocagoz, Adil Allahverdiyev   +6 more
doaj   +4 more sources

Generation of Murine Monoclonal Antibodies by Hybridoma Technology [PDF]

Journal of Visualized Experiments, 2017
Monoclonal antibodies are universal binding molecules and are widely used in biomedicine and research. Nevertheless, the generation of these binding molecules is time-consuming and laborious due to the complicated handling and lack of alternatives. The aim of this protocol is to provide one standard method for the generation of monoclonal antibodies ...
Holzlöhner, Pamela (Dr.), Hanack, Katja (Prof. Dr.)   +1 more
openaire   +3 more sources

Rapid, simple, and effective strategy to produce monoclonal antibodies targeting protein structures using hybridoma technology

Journal of Biological Engineering, 2023
Highlights ・MIHS-SAST screening generates mAbs that target protein structures. ・One-fourth of the mAbs recognized stereospecific epitopes of the protein. ・Two fluorometric parameters aided in selecting antibodies with strong binding constants.
Atsumi Sakaguchi, Yoichiro Tanaka, Eiki Shoji, Teppei Takeshima, Rina Sakamaki, Takao Matsuba, Yasuyuki Kurihara   +6 more
doaj   +2 more sources

Biosafety Aspects of Hybridoma Technology: Nature of Risks and Approaches to Their Management [PDF]

Innovative Biosystems and Bioengineering
This study investigates the biosafety aspects of hybridoma technology, focusing on the identification and management of associated risks. Monoclonal antibodies, essential tools in immunology, biotechnology, and medicine, are primarily produced through ...
Kateryna Shevchuk, Anastasia Baranovska, Andrii Chernetskyi, Nataliia Shchotkina, Alexander Besarab   +4 more
doaj   +2 more sources

Functional Parameters of Hybridoma Cells: Methods of Evaluation and Biotechnological Relevance

Antibodies
Hybridoma technology remains one of the most reliable and widely used platforms for generating highly specific monoclonal antibodies for use in diagnostics, fundamental research, and clinical practice. Moreover, the combination of unlimited proliferative
Tatiana Bezukladnikova, Svetlana Zamorina, Sergey Lazarev, Mikhail Rayev   +3 more
doaj   +2 more sources

Hybridoma technology: new developments of practical interest

Journal of Immunological Methods, 1987
A J Macario
exaly   +3 more sources

Application of recombinant antibodies for treatment of Clostridioides difficile infection: Current status and future perspective

Frontiers in Immunology, 2022
Clostridioides difficile (C. difficile), known as the major cause of antibiotic-associated diarrhea, is regarded as one of the most common healthcare-associated bacterial infections worldwide. Due to the emergence of hypervirulent strains, development of
Hamideh Raeisi, Masoumeh Azimirad, Ali Nabavi-Rad, Hamid Asadzadeh Aghdaei, Abbas Yadegar, Mohammad Reza Zali   +5 more
doaj   +1 more source

Optimal medium use for continuous high density perfusion processes [PDF]

, 1992
Büntemeyer H, Wallerius C, Lehmann J. Optimal medium use for continuous high density perfusion processes. Cytotechnology. 1992;9(1-3):59-67.For maintenance of high cell density in continuous perfusion processes not only feeding with substrates but also ...
Lehmann, Jürgen, Büntemeyer, Heino, Wallerius, Claus   +2 more
core   +1 more source

CRISPR/Cas9-based Engineering of Immunoglobulin Loci in Hybridoma Cells

Bio-Protocol, 2023
Development of the hybridoma technology by Köhler and Milstein (1975) has revolutionized the immunological field by enabling routine use of monoclonal antibodies (mAbs) in research and development efforts, resulting in their successful application in the
Camille Le Gall, Felix Fennemann, Johan van der Schoot, Ferenc Scheeren, Martijn Verdoes   +4 more
doaj   +1 more source

Optimization of serum-free fermentation processes for antibody production [PDF]

, 1991
Büntemeyer H, Lütkemeyer D, Lehmann J. Optimization of serum-free fermentation processes for antibody production. Cytotechnology. 1991;5(1):57-67.Serum free fermentation procedures of cell cultures have got a wide application in production of ...
Lehmann, Jürgen, Büntemeyer, Heino, Lütkemeyer, Dirk   +2 more
core   +1 more source