Results 1 to 10 of about 326 (149)

Hybridoma technology: is it still useful?

Current Research in Immunology, 2021
The isolation of single monoclonal antibodies (mAbs) against a given antigen was only possible with the introduction of the hybridoma technology, which is based on the fusion of specific B lymphocytes with myeloma cells. Since then, several mAbs were described for therapeutic, diagnostic, and research purposes.
Barbara Hamaguchi, Rodrigo Aguiar
exaly   +4 more sources

Generation of Antibodies Selectively Recognizing Epitopes in a Formaldehyde-Fixed Cell-Surface Antigen Using Virus-like Particle Display and Hybridoma Technology

Antibodies, 2023
Efficient induction of target-specific antibodies can be elicited upon immunization with highly immunogenic virus-like particles (VLPs) decorated with desired membrane-anchored target antigens (Ags).
Stefanie Schatz, Lena Willnow, Monika Winkels, Jamila Franca Rosengarten, Benjamin Theek, Ian C. D. Johnston, Jörn Stitz   +6 more
doaj   +3 more sources

Human hybridoma technology

Antibody Technology Journal, 2012
In light of recent developments in the production of human monoclonal antibodies, particularly the method based on isolation of immunoglobulin genes from antigen-specific B cells, the hybridoma technology has become obsolete to some extent. However, the method requires a relatively simple procedure at a low cost and has inherently valuable features ...
Miroslaw Gorny
exaly   +5 more sources

Control of Mycoplasma contamination in hybridoma technology [PDF]

Memorias do Instituto Oswaldo Cruz, 1988
Vera Bongertz, Neide Thomaz
doaj   +4 more sources

Targeted fusion of antibody-secreting cells: Unlocking monoclonal antibody production with hybridoma technology

mAbs
Hybridomas, the first method for creating monoclonal antibodies (mAbs), were reported 50 years ago. This approach, which transformed biomedical research and laid the foundation for many of the current therapeutic, diagnostic, and research reagent ...
Fanny Rousseau, Catherine Menier, Patricia Brochard, Stéphanie Simon, Karla Perez-Toralla, Anne Wijkhuisen   +5 more
doaj   +3 more sources

Production and characterization of antibody against Opisthorchis viverrini via phage display and molecular simulation.

PLoS ONE, 2021
In this study, a key issue to be addressed is the safe disposal of hybridoma instability. Hybridoma technology was used to produce anti-O. viverrini monoclonal antibody.
Sitthinon Siripanthong, Anchalee Techasen, Chanin Nantasenamat, Aijaz Ahmad Malik, Paiboon Sithithaworn, Chanvit Leelayuwat, Amonrat Jumnainsong   +6 more
doaj   +1 more source

Isolation and characterization of full-length genes encoding the anti-human CD45 antibody from the hybridoma cell line 16E8-F2

Ho Chi Minh City Open University Journal of Science - Engineering and Technology, 2022
Though the hybridoma technology has been widely applied in the production of monoclonal antibodies, it has existed some disadvantages including low yield and genetic instability. Therefore, an alternative approach should be taken into account.
Nguyen Quoc Huy, Ta Huong Giang, Nguyen Dang Quan   +2 more
doaj   +1 more source

Chemiluminescent screening of specific hybridoma cells via a proximity-rolling circle activated enzymatic switch

Communications Biology, 2022
In order to realize fast screening of specific hybridoma cells in hybridoma technology, a 1-day chemiluminescent screening method is reported on common 96-well plate via a proximity-rolling circle activated enzymatic switch strategy.
Hang Ao, Weiwei Chen, Jie Wu, Wencheng Xiao, Huangxian Ju   +4 more
doaj   +1 more source

Hybridoma technology; advancements, clinical significance, and future aspects

Journal of Genetic Engineering and Biotechnology, 2021
Background Hybridoma technology is one of the most common methods used to produce monoclonal antibodies. In this process, antibody-producing B lymphocytes are isolated from mice after immunizing the mice with specific antigen and are fused with immortal ...
Sanchita Mitra, Pushpa Chaudhary Tomar
doaj   +1 more source

Substantially Improved Electrofusion Efficiency of Hybridoma Cells: Based on the Combination of Nanosecond and Microsecond Pulses

Bioengineering, 2022
As the initial antibody technology, the preparation of hybridoma cells has been widely used in discovering antibody drugs and is still in use. Various antibody drugs obtained through this technology have been approved for treating human diseases. However,
Meng Wu, Qiang Ke, Jinhao Bi, Xinhao Li, Shuheng Huang, Zuohua Liu, Liangpeng Ge   +6 more
doaj   +1 more source