Results 1 to 10 of about 59,780 (293)
Human endothelial culture supernatant (HECS): a growth factor for hybridomas. [PDF]
Human endothelial culture supernatant (HECS) had strong growth-promotion activity for hybridoma cells: the yield of hybridomas after fusion was increased at least 2-fold over that in the presence of feeder cells, such as mouse macrophages and spleen ...
G Astaldi+5 more
openalex +2 more sources
Production of a T cell hybridoma that expresses the T cell receptor gamma/delta heterodimer. [PDF]
We have produced a T cell hybridoma line by fusion of an IL-2-dependent, long-term T cell receptor (TCR) gamma/delta+ Thy-1+, bone marrow-derived, dendritic epidermal cell line to the BW5147 tumor line.
Wayne M. Yokoyama+5 more
openalex +4 more sources
Antigen-specific identification and cloning of hybridomas with a fluorescence-activated cell sorter.
David R. Parks+3 more
openalex +2 more sources
Monoclonal antibodies are commonly assumed to be monospecific, but anecdotal studies have reported genetic diversity in antibody heavy chain and light chain genes found within individual hybridomas.
A. Bradbury+24 more
semanticscholar +1 more source
Characterization of the T cell receptor repertoire causing collagen arthritis in mice [PDF]
Collagen type II-induced arthritis (CIA) is generated in susceptible rodent strains by intradermal injections of homologous or heterologous native type II collagen in complete Freund's adjuvant.
Hood, Leroy E.+3 more
core +2 more sources
We developed a method for production of antigen-specific, H-2-restricted T cell hybrids. The tumor cell partner in the fusions was itself a T cell hybrid, FS6-14.13.AG2 (or its derivatives), which could be induced to produce the growth factor ...
J. Kappler+3 more
semanticscholar +1 more source
Hybrid cell lines producing monoclonal antibodies directed against Treponema [PDF]
Continuous hybrid cell lines for producing monoclonal antibodies directed against antigens of Treponema pallidum have been developed. The hybrid cell lines were established by fusing differentiated lymphoid cells primed with antigens of Treponema ...
Kettman, John R., Norgard, Michael V.
core +1 more source
Monoclonal antibodies against human astrocytomas and their reactivity pattern [PDF]
The establishment of hybridomas after fusion of X63-Ag8.653 mouse myeloma cells and splenocytes from mice hyperimmunized against human astrocytomas is presented. The animals were primed with 5 × 106 chemically modified uncultured or cultured glioma cells.
B. Martin+36 more
core +1 more source
Class-switched anti-insulin antibodies originate from unconventional antigen presentation in multiple lymphoid sites [PDF]
Autoantibodies to insulin are a harbinger of autoimmunity in type 1 diabetes in humans and in non-obese diabetic mice. To understand the genesis of these autoantibodies, we investigated the interactions of insulin-specific T and B lymphocytes using T ...
Acevedo-Suárez+59 more
core +2 more sources
A rat monoclonal antibody specific for immunoglobulin (Ig) heavy chain binding protein (BiP) has allowed the examination of the association of BiP with assembling Ig precursors in mouse B lymphocyte-derived cell lines.
D. Bole, L. Hendershot, J. Kearney
semanticscholar +1 more source