Results 1 to 10 of about 55,509 (296)

Targeted fusion of antibody-secreting cells: Unlocking monoclonal antibody production with hybridoma technology [PDF]

mAbs
Hybridomas, the first method for creating monoclonal antibodies (mAbs), were reported 50 years ago. This approach, which transformed biomedical research and laid the foundation for many of the current therapeutic, diagnostic, and research reagent ...
Fanny Rousseau, Catherine Menier, Patricia Brochard, Stéphanie Simon, Karla Perez-Toralla, Anne Wijkhuisen   +5 more
doaj   +2 more sources

Identification and validation of genes encoding humoral specificity for the chemical allergen toluene diisocyanate. [PDF]

PLoS ONE
A panel of hybridomas specific for different isomers of toluene diisocyanate (TDI), a cross‑linking chemical used in polyurethane production, has been previously described.
Adam V Wisnewski, Jian Liu
doaj   +2 more sources

Virus-like Particles and Spectral Flow Cytometry for Identification of Dengue Virus-Specific B Cells in Mice and Humans [PDF]

Viruses
Severe dengue virus (DENV) infections are associated with circulating non-neutralizing antibodies generated during heterotypic infections. Although antibodies are key mediators of both protection and pathogenesis, the specific dynamics of B cells (Bc ...
Katherine Segura, Fabiola Martel, Manuel A. Franco, Federico Perdomo-Celis, Carlos F. Narváez   +4 more
doaj   +2 more sources

Novel BCR-Targeting Fusion Proteins for Antigen-Specific Depletion of Alloreactive B Cells in Antibody-Mediated Rejection [PDF]

Cells
Donor-specific anti-HLA antibodies (DSAs) bind to donor vascular endothelial cells and mediate allograft rejection (AMR), but a clinical challenge for which targeted therapeutic options remain limited.
Jing Zhang, Leiyan Wei, Lei Song, Xiaofang Lu, Liang Tan, Xin Li, Li Fu, Qizhi Luo, Xubiao Xie, Yizhou Zou   +9 more
doaj   +2 more sources

Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies against Coronavirus SARS-CоV-2

Проблемы особо опасных инфекций, 2023
The aim of the work was to obtain and characterize hybridomas producing monoclonal antibodies to antigens of coronavirus SARS‑CoV‑2, promising for the construction of diagnostic immunochemical tests. Materials and methods.
G. V. Kuklina, S. S. Ipatov, A. S. Gorshkov, D. V. Pechenkin, A. V. Eremkin, A. V. Kuznetsovsky, A. S. Tumanov, I. V. Darmov   +7 more
doaj   +1 more source

Study on the Antibody Production Efficiency in Modified Big-BALB/c Mice

Shiyan dongwu yu bijiao yixue, 2023
Objective To compare the preparation efficiency of mouse pox and mouse hepatitis antibodies between two substrains of BALB/c and Big-BALB/c (B-BALB/c) mice, and to provide a theoretical basis and reference for the selection of laboratory animals in the ...
WANG Dan, ZHANG Xiaolu, WANG Yan, FU Bo, WANG Wendong, LIU Jing, ZHANG Suyin, WU Yihe, WU Deguo, DU Xiaoyan, ZHAN Dawei, ZHANG Xiulin, LI Changlong   +12 more
doaj   +1 more source

Identification of Immunoglobulin Gene Sequences from a Small Read Number of mRNA-Seq Using Hybridomas. [PDF]

PLoS ONE, 2016
Identification of immunoglobulin genes in hybridomas is essential for producing antibodies for research and clinical applications. A couple of methods such as RACE and degenerative PCR have been developed for determination of the Igh and Igl/Igk coding ...
Yuki Kuniyoshi, Kazumitsu Maehara, Takeshi Iwasaki, Masayasu Hayashi, Yuichiro Semba, Masatoshi Fujita, Yuko Sato, Hiroshi Kimura, Akihito Harada, Yasuyuki Ohkawa   +9 more
doaj   +1 more source

Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies to Shiga-Like Toxins of I and II Types

Проблемы особо опасных инфекций, 2021
Objective – obtaining and characterization of hybrid cell lines producing monoclonal antibodies against I and II types of shiga-like toxins.Materials and methods.
G. V. Kuklina, S. S. Ipatov, D. V. Pechenkin, A. V. Eremkin, A. A. Kytmanov, M. Yu. Dubrovin, A. S. Gorshkov   +6 more
doaj   +1 more source

When monoclonal antibodies are not monospecific: Hybridomas frequently express additional functional variable regions

mAbs, 2018
Monoclonal antibodies are commonly assumed to be monospecific, but anecdotal studies have reported genetic diversity in antibody heavy chain and light chain genes found within individual hybridomas.
A. Bradbury, N. Trinklein, H. Thie, Ian C. Wilkinson, A. Tandon, S. Anderson, C. L. Bladen, Brittany Jones, S. Aldred, M. Bestagno, O. Burrone, J. Maynard, F. Ferrara, J. Trimmer, Janina Görnemann, J. Glanville, P. Wolf, A. Frenzel, J. Wong, Xin Yu Koh, Hui-Yan Eng, David P. Lane, M. Lefranc, M. Clark, S. Dübel   +24 more
semanticscholar   +1 more source

Characterization of the T cell receptor repertoire causing collagen arthritis in mice [PDF]

, 1993
Collagen type II-induced arthritis (CIA) is generated in susceptible rodent strains by intradermal injections of homologous or heterologous native type II collagen in complete Freund's adjuvant.
Hood, Leroy E., Kanagawa, Osami, Osman, Gamal-E, Toda, Masaaki   +3 more
core   +2 more sources