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Targeted fusion of antibody-secreting cells: Unlocking monoclonal antibody production with hybridoma technology [PDF]

mAbs
Hybridomas, the first method for creating monoclonal antibodies (mAbs), were reported 50 years ago. This approach, which transformed biomedical research and laid the foundation for many of the current therapeutic, diagnostic, and research reagent ...
Fanny Rousseau, Catherine Menier, Patricia Brochard, Stéphanie Simon, Karla Perez-Toralla, Anne Wijkhuisen   +5 more
doaj   +2 more sources

Production of a T cell hybridoma that expresses the T cell receptor gamma/delta heterodimer. [PDF]

, 1987
We have produced a T cell hybridoma line by fusion of an IL-2-dependent, long-term T cell receptor (TCR) gamma/delta+ Thy-1+, bone marrow-derived, dendritic epidermal cell line to the BW5147 tumor line.
Bluestone, JA, Coligan, JE, Koning, F, Shevach, EM, Stingl, G, Yokoyama, WM   +5 more
core   +3 more sources

Development of monoclonal antibodies against Rhodococcus equi virulence-associated protein N and their application to pathological diagnosis [PDF]

Microbiology Spectrum, 2023
Since 2015, virulence-associated protein N (vapN)-positive Rhodococcus equi has been isolated from pyogenic lesions in ruminants in multiple countries, including Japan, supporting the widespread distribution of this pathogen in ruminants, and suggesting ...
Yasunori Suzuki, Shinji Takai, Yuri Morizane, Kentaro Yasuda, Kei Takahashi, Toko Ishitsuka, Yukako Sasaki, Mikihiro Otsuka, Satoru Kato, Hiroo Madarame, Makoto Sugiyama, Hiroaki Kawaguchi, Tsutomu Kakuda   +12 more
doaj   +2 more sources

Development and Evaluation of Monoclonal Antibodies against CBPP Antigen with the End Goal of Developing an ELISA Kit [PDF]

Veterinary Medicine International
Contagious bovine pleuropneumonia (CBPP) is an infectious and contagious bacterial respiratory disease that affects cattle with significant economic losses to the African animal industry.
Lorato Ramathudi-Dunbar, Emmanuel Awosanya, Sanne Bodjo Charles, Ethel Chitsungo, Cisse Rahamatou Moustapha Boukary, Nick Nwankpa, Hassen Gelaw, Yebechaye Tessema, Gelagay Melesse A., Richard Rayson Sanga, Adorbley Bright, Jean de Dieu Baziki   +11 more
doaj   +2 more sources

Study on the Antibody Production Efficiency in Modified Big-BALB/c Mice

Shiyan dongwu yu bijiao yixue, 2023
Objective To compare the preparation efficiency of mouse pox and mouse hepatitis antibodies between two substrains of BALB/c and Big-BALB/c (B-BALB/c) mice, and to provide a theoretical basis and reference for the selection of laboratory animals in the ...
WANG Dan, ZHANG Xiaolu, WANG Yan, FU Bo, WANG Wendong, LIU Jing, ZHANG Suyin, WU Yihe, WU Deguo, DU Xiaoyan, ZHAN Dawei, ZHANG Xiulin, LI Changlong   +12 more
doaj   +1 more source

Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies against Coronavirus SARS-CоV-2

Проблемы особо опасных инфекций, 2023
The aim of the work was to obtain and characterize hybridomas producing monoclonal antibodies to antigens of coronavirus SARS‑CoV‑2, promising for the construction of diagnostic immunochemical tests. Materials and methods.
G. V. Kuklina, S. S. Ipatov, A. S. Gorshkov, D. V. Pechenkin, A. V. Eremkin, A. V. Kuznetsovsky, A. S. Tumanov, I. V. Darmov   +7 more
doaj   +1 more source

Identification of Immunoglobulin Gene Sequences from a Small Read Number of mRNA-Seq Using Hybridomas. [PDF]

PLoS ONE, 2016
Identification of immunoglobulin genes in hybridomas is essential for producing antibodies for research and clinical applications. A couple of methods such as RACE and degenerative PCR have been developed for determination of the Igh and Igl/Igk coding ...
Yuki Kuniyoshi, Kazumitsu Maehara, Takeshi Iwasaki, Masayasu Hayashi, Yuichiro Semba, Masatoshi Fujita, Yuko Sato, Hiroshi Kimura, Akihito Harada, Yasuyuki Ohkawa   +9 more
doaj   +1 more source

Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies to Shiga-Like Toxins of I and II Types

Проблемы особо опасных инфекций, 2021
Objective – obtaining and characterization of hybrid cell lines producing monoclonal antibodies against I and II types of shiga-like toxins.Materials and methods.
G. V. Kuklina, S. S. Ipatov, D. V. Pechenkin, A. V. Eremkin, A. A. Kytmanov, M. Yu. Dubrovin, A. S. Gorshkov   +6 more
doaj   +1 more source

PRODUÇÃO DE HIBRIDOMAS SECRETORES DE ANTICORPOS ANTI- Neospora caninum PARA USO EM IMUNODIAGNÓSTICO

Ciência Animal Brasileira, 2014
The Neospora caninum is a protozoan Apicomplexa with greater involvement in abortions worldwide. The economic losses determined by neosporosis also include abortions besides the early disposal of cows, costs for replacing animals in the herd, drop in ...
Bruna Alves Devens, Marlene Isabel Vargas Viloria, Carlos Henryque de Souza e Silva, Joaquin Hernán Patarroyo Salcedo   +3 more
doaj   +1 more source

Production and characterisation of monoclonal antibodies for the detection of AOZ, a tissue bound metabolite of furazolidone

Veterinární Medicína, 2005
3-amino-2-oxazolidinone (AOZ) is a tissue bound toxic metabolite derived from the nitrofuran antibiotic, furazolidone. AOZ is detected in the derivatised form of 3-{[(2-nitrophenyl) methylene] amino}-2-oxa-zolidinone (NP AOZ).
M. Vass, L. Kotkova, I. Diblikova, Z. Nevorankova, K.M. Cooper, D.G. Kennedy, M. Franek   +6 more
doaj   +1 more source