Results 1 to 10 of about 5,442 (155)

Study on the Antibody Production Efficiency in Modified Big-BALB/c Mice

Shiyan dongwu yu bijiao yixue, 2023
Objective To compare the preparation efficiency of mouse pox and mouse hepatitis antibodies between two substrains of BALB/c and Big-BALB/c (B-BALB/c) mice, and to provide a theoretical basis and reference for the selection of laboratory animals in the ...
WANG Dan, ZHANG Xiaolu, WANG Yan, FU Bo, WANG Wendong, LIU Jing, ZHANG Suyin, WU Yihe, WU Deguo, DU Xiaoyan, ZHAN Dawei, ZHANG Xiulin, LI Changlong   +12 more
doaj   +1 more source

Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies against Coronavirus SARS-CоV-2

Проблемы особо опасных инфекций, 2023
The aim of the work was to obtain and characterize hybridomas producing monoclonal antibodies to antigens of coronavirus SARS‑CoV‑2, promising for the construction of diagnostic immunochemical tests. Materials and methods.
G. V. Kuklina, S. S. Ipatov, A. S. Gorshkov, D. V. Pechenkin, A. V. Eremkin, A. V. Kuznetsovsky, A. S. Tumanov, I. V. Darmov   +7 more
doaj   +1 more source

Identification of Immunoglobulin Gene Sequences from a Small Read Number of mRNA-Seq Using Hybridomas. [PDF]

PLoS ONE, 2016
Identification of immunoglobulin genes in hybridomas is essential for producing antibodies for research and clinical applications. A couple of methods such as RACE and degenerative PCR have been developed for determination of the Igh and Igl/Igk coding ...
Yuki Kuniyoshi, Kazumitsu Maehara, Takeshi Iwasaki, Masayasu Hayashi, Yuichiro Semba, Masatoshi Fujita, Yuko Sato, Hiroshi Kimura, Akihito Harada, Yasuyuki Ohkawa   +9 more
doaj   +1 more source

Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies to Shiga-Like Toxins of I and II Types

Проблемы особо опасных инфекций, 2021
Objective – obtaining and characterization of hybrid cell lines producing monoclonal antibodies against I and II types of shiga-like toxins.Materials and methods.
G. V. Kuklina, S. S. Ipatov, D. V. Pechenkin, A. V. Eremkin, A. A. Kytmanov, M. Yu. Dubrovin, A. S. Gorshkov   +6 more
doaj   +1 more source

PRODUÇÃO DE HIBRIDOMAS SECRETORES DE ANTICORPOS ANTI- Neospora caninum PARA USO EM IMUNODIAGNÓSTICO

Ciência Animal Brasileira, 2014
The Neospora caninum is a protozoan Apicomplexa with greater involvement in abortions worldwide. The economic losses determined by neosporosis also include abortions besides the early disposal of cows, costs for replacing animals in the herd, drop in ...
Bruna Alves Devens, Marlene Isabel Vargas Viloria, Carlos Henryque de Souza e Silva, Joaquin Hernán Patarroyo Salcedo   +3 more
doaj   +1 more source

Emerging roles of BET proteins in transcription and co‐transcriptional RNA processing

WIREs RNA, Volume 14, Issue 1, January/February 2023., 2023
Roles of BET proteins in RNA polymerase II transcription and co‐transcriptional RNA processing. Abstract Transcription by RNA polymerase II (Pol II) gives rise to all nuclear protein‐coding and a large set of non‐coding RNAs, and is strictly regulated and coordinated with RNA processing.
Nicole Eischer, Mirjam Arnold, Andreas Mayer   +2 more
wiley   +1 more source

Production and characterisation of monoclonal antibodies for the detection of AOZ, a tissue bound metabolite of furazolidone

Veterinární Medicína, 2005
3-amino-2-oxazolidinone (AOZ) is a tissue bound toxic metabolite derived from the nitrofuran antibiotic, furazolidone. AOZ is detected in the derivatised form of 3-{[(2-nitrophenyl) methylene] amino}-2-oxa-zolidinone (NP AOZ).
M. Vass, L. Kotkova, I. Diblikova, Z. Nevorankova, K.M. Cooper, D.G. Kennedy, M. Franek   +6 more
doaj   +1 more source

Manufacturing of hybridomas, producing monoclonal antibodies against Burkholderia mallei and Burkholderia pseudomallei antigens

Журнал микробиологии, эпидемиологии и иммунобиологии, 2019
Aim. Obtaining hybridomas, stable producing specific monoclonal antibodies against Burkholderia mallei and Burkholderia pseudomallei antigens. Materials and methods.
G. V. Kuklina, G. D. Elagin, D. V. Pechenkin, O. O. Fomenkov, A. V. Eremkin, A. A. Kytmanov, S. A. Shurupov, S. S. Ipatov   +7 more
doaj   +1 more source

Obtaining and Characterization of the Monoclonal Antibodies Against G-Protein of the Respiratory Syncytial Virus

Журнал микробиологии, эпидемиологии и иммунобиологии, 2020
The aim of this study was to obtain hybridomas producing monoclonal antibodies (Mabs) to the G-protein of the respiratory syncytial virus (RSV), and to evaluate their immunological characteristics and virus-neutralizing activity.Material and methods ...
N. A. Demidova, R. R. Klimova, A. A. Kushch, E. I. Lesnova, O. V. Masalova, A. D. Dorofeeva, A. A. Nikonova, N. E. Fedorova, V. V. Zverev   +8 more
doaj   +1 more source

Diversity of the antibody response to tetanus toxoid: comparison of hybridoma library to phage display library. [PDF]

PLoS ONE, 2014
Monoclonal antibodies are important tools in research and since the 1990s have been an important therapeutic class targeting a wide variety of diseases. Earlier methods of mAb production relied exclusively on the lengthy process of making hybridomas. The
Mahsa Sorouri, Sean P Fitzsimmons, Antonina G Aydanian, Sonita Bennett, Marjorie A Shapiro   +4 more
doaj   +1 more source