Development of monoclonal antibodies against Rhodococcus equi virulence-associated protein N and their application to pathological diagnosis [PDF]
Microbiology Spectrum, 2023 Since 2015, virulence-associated protein N (vapN)-positive Rhodococcus equi has been isolated from pyogenic lesions in ruminants in multiple countries, including Japan, supporting the widespread distribution of this pathogen in ruminants, and suggesting ...
Yasunori Suzuki +12 more
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Efficient Cross-Screening and Characterization of Monoclonal Antibodies against Marek’s Disease Specific Meq Oncoprotein Using CRISPR/Cas9-Gene-Edited Viruses [PDF]
Viruses, 2023 Marek’s disease (MD) caused by pathogenic Marek’s disease virus type 1 (MDV−1) is one of the most important neoplastic diseases of poultry. MDV−1-encoded unique Meq protein is the major oncoprotein and the availability of Meq-specific monoclonal ...
Man Teng +7 more
doaj +2 more sources
Expression of a recombinant ASFV P30 protein and production of monoclonal antibodies [PDF]
Open Veterinary Journal, 2023 Background: African Swine Fever (ASF) is an infectious disease that affects domestic pig and wild boar populations. The African Swine Fever Virus (ASFV) has a genome characterized by a very complex DNA (170-193 kb) that encodes for more than 200 ...
Rosrio Liberti +10 more
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Development and Evaluation of Monoclonal Antibodies against CBPP Antigen with the End Goal of Developing an ELISA Kit [PDF]
Veterinary Medicine InternationalContagious bovine pleuropneumonia (CBPP) is an infectious and contagious bacterial respiratory disease that affects cattle with significant economic losses to the African animal industry.
Lorato Ramathudi-Dunbar +11 more
doaj +2 more sources
A monoclonal antibody collection for C. difficile typing ? [PDF]
Gut PathogensClostridioides difficile is the leading cause of antibiotic-associated diarrhea and pseudomembranous colitis in adults. Various C. difficile strains circulate currently, associated with different outcomes and antibiotic resistance profiles. However, most
Lise Hunault +12 more
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The CD4 transmembrane GGXXG and juxtamembrane (C/F)CV+C motifs mediate pMHCII-specific signaling independently of CD4-LCK interactions [PDF]
eLifeCD4+ T cell activation is driven by five-module receptor complexes. The T cell receptor (TCR) is the receptor module that binds composite surfaces of peptide antigens embedded within MHCII molecules (pMHCII).
Mark S Lee +7 more
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Проблемы особо опасных инфекций, 2023 The aim of the work was to obtain and characterize hybridomas producing monoclonal antibodies to antigens of coronavirus SARS‑CoV‑2, promising for the construction of diagnostic immunochemical tests. Materials and methods.
G. V. Kuklina +7 more
doaj +1 more source
Study on the Antibody Production Efficiency in Modified Big-BALB/c Mice
Shiyan dongwu yu bijiao yixue, 2023 Objective To compare the preparation efficiency of mouse pox and mouse hepatitis antibodies between two substrains of BALB/c and Big-BALB/c (B-BALB/c) mice, and to provide a theoretical basis and reference for the selection of laboratory animals in the ...
WANG Dan +12 more
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Identification of Immunoglobulin Gene Sequences from a Small Read Number of mRNA-Seq Using Hybridomas. [PDF]
PLoS ONE, 2016 Identification of immunoglobulin genes in hybridomas is essential for producing antibodies for research and clinical applications. A couple of methods such as RACE and degenerative PCR have been developed for determination of the Igh and Igl/Igk coding ...
Yuki Kuniyoshi +9 more
doaj +1 more source
Проблемы особо опасных инфекций, 2021 Objective – obtaining and characterization of hybrid cell lines producing monoclonal antibodies against I and II types of shiga-like toxins.Materials and methods.
G. V. Kuklina +6 more
doaj +1 more source