Results 11 to 20 of about 59,735 (325)

Antigen-specific identification and cloning of hybridomas with a fluorescence-activated cell sorter.

Proceedings of the National Academy of Sciences of the United States of America, 1979
Myeloma-spleen cell hybrids (hybridomas) producing antibody to mouse immunoglobulin allotypes have been labeled with fluorescent microspheres coupled with myeloma protein antigens.
David R. Parks, V M Bryan, Vernon T. Oi, L A Herzenberg   +3 more
openalex   +2 more sources

Human endothelial culture supernatant (HECS): a growth factor for hybridomas. [PDF]

Journal of Immunology, 1980
Human endothelial culture supernatant (HECS) had strong growth-promotion activity for hybridoma cells: the yield of hybridomas after fusion was increased at least 2-fold over that in the presence of feeder cells, such as mouse macrophages and spleen ...
G Astaldi, M C Janssen, Peter M. Lansdorp, C Willems, W.P. Zeijlemaker, F. Oosterhof   +5 more
openalex   +2 more sources

When monoclonal antibodies are not monospecific: Hybridomas frequently express additional functional variable regions

mAbs, 2018
Monoclonal antibodies are commonly assumed to be monospecific, but anecdotal studies have reported genetic diversity in antibody heavy chain and light chain genes found within individual hybridomas.
A. Bradbury, N. Trinklein, H. Thie, Ian C. Wilkinson, A. Tandon, S. Anderson, C. Bladen, Brittany Jones, S. F. Aldred, M. Bestagno, O. Burrone, J. Maynard, F. Ferrara, J. Trimmer, Janina Görnemann, J. Glanville, P. Wolf, A. Frenzel, J. Wong, Xin Yu Koh, Hui-Yan Eng, David P. Lane, M. Lefranc, M. Clark, S. Dübel   +24 more
semanticscholar   +1 more source

Antigen-inducible, H-2-restricted, interleukin-2-producing T cell hybridomas. Lack of independent antigen and H-2 recognition

Journal of Experimental Medicine, 1981
We developed a method for production of antigen-specific, H-2-restricted T cell hybrids. The tumor cell partner in the fusions was itself a T cell hybrid, FS6-14.13.AG2 (or its derivatives), which could be induced to produce the growth factor ...
J. Kappler, B. Skidmore, J. White, P. Marrack   +3 more
semanticscholar   +1 more source

Obtaining Specific Hybridomas for Ki-67 Protein Immunodetection

Indonesian Biomedical Journal, 2021
BACKGROUND: Active proliferation is specific property of a tumor cells. However, the cost of the analysis is high due to commercial anti-Ki-67 mAbs used as the main immunoreagent for reliable identification of proliferating cells.
Aigerim Turgimbayeva, Sailau Abeldenov, Nurgul Sarina, Bekbolat Khassenov, Saule Eskendirova   +4 more
doaj   +1 more source

Hybrid cell lines producing monoclonal antibodies directed against Treponema [PDF]

, 1985
Continuous hybrid cell lines for producing monoclonal antibodies directed against antigens of Treponema pallidum have been developed. The hybrid cell lines were established by fusing differentiated lymphoid cells primed with antigens of Treponema ...
Kettman, John R., Norgard, Michael V.
core   +1 more source

Manufacturing of hybridomas, producing monoclonal antibodies against Burkholderia mallei and Burkholderia pseudomallei antigens

Журнал микробиологии, эпидемиологии и иммунобиологии, 2019
Aim. Obtaining hybridomas, stable producing specific monoclonal antibodies against Burkholderia mallei and Burkholderia pseudomallei antigens. Materials and methods.
G. V. Kuklina, G. D. Elagin, D. V. Pechenkin, O. O. Fomenkov, A. V. Eremkin, A. A. Kytmanov, S. A. Shurupov, S. S. Ipatov   +7 more
doaj   +1 more source

Diversity of the antibody response to tetanus toxoid: comparison of hybridoma library to phage display library. [PDF]

PLoS ONE, 2014
Monoclonal antibodies are important tools in research and since the 1990s have been an important therapeutic class targeting a wide variety of diseases. Earlier methods of mAb production relied exclusively on the lengthy process of making hybridomas. The
Mahsa Sorouri, Sean P Fitzsimmons, Antonina G Aydanian, Sonita Bennett, Marjorie A Shapiro   +4 more
doaj   +1 more source

Monoclonal antibodies against human astrocytomas and their reactivity pattern [PDF]

, 1987
The establishment of hybridomas after fusion of X63-Ag8.653 mouse myeloma cells and splenocytes from mice hyperimmunized against human astrocytomas is presented. The animals were primed with 5 × 106 chemically modified uncultured or cultured glioma cells.
B. Martin, Bourdon, Bullard, Bullard, Carrel, Coakham, Coakham, D. Stavrou, De Muralt, E. Keiditsch, Epenetos, F. Schmidberger, I. Funke, Jonak, K. Bise, Kearney, Kemshead, Krajewski, Köhler, Lanier, Posner, R. Kurrle, Roholl, Schnegg, Seeger, Sikora, Stavrou, Stavrou, Stavrou, Stavrou, Stavrou, Stavrou, Stavrou, Taub, U. Stocker, W. Eisenmenger, Wikstrand   +36 more
core   +1 more source

Production and characterisation of monoclonal antibodies for the detection of AOZ, a tissue bound metabolite of furazolidone

Veterinární Medicína, 2005
3-amino-2-oxazolidinone (AOZ) is a tissue bound toxic metabolite derived from the nitrofuran antibiotic, furazolidone. AOZ is detected in the derivatised form of 3-{[(2-nitrophenyl) methylene] amino}-2-oxa-zolidinone (NP AOZ).
M. Vass, L. Kotkova, I. Diblikova, Z. Nevorankova, K.M. Cooper, D.G. Kennedy, M. Franek   +6 more
doaj   +1 more source