Results 31 to 40 of about 45,417 (262)

Fluorescent Microtiter Screening Assay for Immunocytochemically Reactive Antibodies

BioTechniques, 1997
We have developed a rapid in situ screening procedure that enables prescreening of hundreds of hybridomas in a 96-well format. The procedure involves fluorescence immunostaining of cells cultured in 96-well plates and the use of a fluorescence plate ...
J.-L. Su, K.E. Kilpatrick, B.R. Champion, D.C. Morris, J.M. Lehmann, T.A. Kost   +5 more
doaj   +1 more source

High-throughput single-cell screening of viable hybridomas and patient-derived antibody-secreting cells using punchable microwells

Artificial Cells, Nanomedicine, and Biotechnology
Monoclonal antibodies (mAbs) hold significant potential as therapeutic agents and are invaluable tools in biomedical research. However, the lack of efficient high-throughput screening methods for single antibody-secreting cells (ASCs) has limited the ...
Kaat Rubben, Ann-Sophie Vander Plaetsen, Ruben Almey, Olivier Tytgat, Koen Deserranno, Jamie Debaere, Delphine Diana Acar, Philip Meuleman, Dieter Deforce, Filip Van Nieuwerburgh   +9 more
doaj   +1 more source

Manufacturing of Hybridomas-Producers of Monoclonal Anti-Bodies to Tularemia Agent Antigens

Проблемы особо опасных инфекций, 2015
Carried out have been two experimental studies on hybridization of mouse myeloma cells and lymphocytes of BALB/c mice immunized with inactivated microbe Francisella tularensis cultures.
O. O. Fomenkov, G. D. Elagin, G. V. Kuklina, D. V. Pechenkin, V. V. Krupin, N. V. Bogacheva, A. A. Kytmanov, A. V. Eremkin, O. V. Tikhvinskaya, E. Yu. Vakhnov   +9 more
doaj   +1 more source

Multiplexed Quantitation of Intraphagocyte Mycobacterium tuberculosis Secreted Protein Effectors

Cell Reports, 2018
Summary: The pathogenic potential of Mycobacterium tuberculosis largely depends on ESX secretion systems exporting members of the multigenic Esx, Esp, and PE/PPE protein families.
Fadel Sayes, Catherine Blanc, Louis S. Ates, Nathalie Deboosere, Mickael Orgeur, Fabien Le Chevalier, Matthias I. Gröschel, Wafa Frigui, Ok-Ryul Song, Richard Lo-Man, Florence Brossier, Wladimir Sougakoff, Daria Bottai, Priscille Brodin, Pierre Charneau, Roland Brosch, Laleh Majlessi   +16 more
doaj   +1 more source

Two better cell lines for making hybridomas expressing specific T cell receptors.

Journal of Immunology, 1989
Two variants of the AKR thymoma BW5147 have been isolated which can no longer express functional TCR alpha- and beta-chains. By generating hybridomas with these variant fusion lines, TCR of any normal T lymphocyte, including TCR-gamma/delta, can be ...
Janicé White, M. Blackman, J. Bill, J. Kappler, P. Marrack, D. Gold, W. Born   +6 more
semanticscholar   +1 more source

Naked cuticle is essential for Drosophila wing development beyond Wingless signaling

FEBS Open Bio, EarlyView.
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley   +1 more source

Fas and activation-induced Fas ligand mediate apoptosis of T cell hybridomas: inhibition of Fas ligand expression by retinoic acid and glucocorticoids

Journal of Experimental Medicine, 1995
Activation of T cell hybridomas induces a G1/S cell cycle block and apoptosis. We isolated a variant of the 2B4.11 T cell hybridoma that, when activated via the TCR, produced IL-2 and underwent growth inhibition but did not die.
Yili Yang, M. Merćep, C. Ware, J. Ashwell   +3 more
semanticscholar   +1 more source

Visual Recovery Reflects Cortical MeCP2 Sensitivity in Rett Syndrome

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Rett syndrome (RTT) is a devastating neurodevelopmental disorder with developmental regression affecting motor, sensory, and cognitive functions. Sensory disruptions contribute to the complex behavioral and cognitive difficulties and represent an important target for therapeutic interventions.
Alex Joseph Simon, Nathalie Picard, Valeria d'Andrea, Enchi Chang, Joseph Leffler, Eleonora Centofante, Matthew Taylor, Francesca Bardi, Francesca Cavicchiolo, Takao K. Hensch, Stefano Panzeri, Chinfei Chen, Michela Fagiolini   +12 more
wiley   +1 more source

Multivalent Protein Nanorings for Broad and Potent SARS‐CoV‐2 Neutralization

Advanced Healthcare Materials, EarlyView.
A protein‐only, modular multivalent nanoscaffold displaying 20 anchor points, decorated with two different binders (10 of each), targeting the SARS‐CoV‐2 receptor‐binding domain is presented. The construct self‐assembles into stable, biocompatible, homogeneous nanoparticles, exhibit synergistic binding with fM IC50 values. It also detects spike at 9 ng 
Molood Behbahanipour, Carla Goldin, Cecilia Arahi Prato, Ana Luz Paletta, Oriol Bárcenas, Susanna Navarro, Jordi Pujols, Daniel H. Murgida, María Virginia Tribulatti, Sebastián Esperante, Damian Alvarez‐Paggi, Salvador Ventura   +11 more
wiley   +1 more source

A New Strategy for Efficient Screening and Identification of Monoclonal Antibodies against Oncogenic Avian Herpesvirus Utilizing CRISPR/Cas9-Based Gene-Editing Technology

Viruses, 2022
Marek’s disease virus (MDV) is an important oncogenic α-herpesvirus that induces Marek’s disease (MD), characterized by severe immunosuppression and rapid-onset T-cell lymphomas in its natural chicken hosts.
Man Teng, Zi-Yu Zhou, Yongxiu Yao, Venugopal Nair, Gai-Ping Zhang, Jun Luo   +5 more
doaj   +1 more source