Results 31 to 40 of about 55,509 (296)

A hybridoma-derived monoclonal antibody with high homology to the aberrant myeloma light chain.

open access: yesPLoS ONE, 2021
The identification of antibody variable regions in the heavy (VH) and light (VL) chains from hybridomas is necessary for the production of recombinant, sequence-defined monoclonal antibodies (mAbs) and antibody derivatives.
Ghasidit Pornnoppadol   +6 more
doaj   +1 more source

Polymeric Approach to Adjuvant System in Antibody Production against Leishmaniasis Based on Hybridoma Technology

open access: yesIranian Journal of Parasitology, 2022
Background: Leishmaniasis is a zoonotic disease, which is one of the serious public health problems in the world. Nowadays, antibody production using hybridoma technology may be a correct approach in terms of sensitivity in the diagnosis of diseases ...
Asli Pinar Zorba Yildiz   +6 more
doaj   +1 more source

Preparation and Biological Activity of the Monoclonal Antibody against the Second Extracellular Loop of the Angiotensin II Type 1 Receptor

open access: yesJournal of Immunology Research, 2016
The current study was to prepare a mouse-derived antibody against the angiotensin II type 1 receptor (AT1-mAb) based on monoclonal antibody technology, to provide a foundation for research on AT1-AA-positive diseases.
Mingming Wei   +5 more
doaj   +1 more source

Utilization of Immunoblotting in Studies of Epitope Targeting in Monoclonal Antibodies to Melioidosis Agent Antigen 200 kDa

open access: yesПроблемы особо опасных инфекций, 2014
Objective of the research was to use immunoblotting for studies of epitope targeting in monoclonal antibodies to 200 kDa Burkholderia pseudomallei antigen, which are synthesized by hybridomas-producers from the two collections in the laboratory of ...
I. I. Korsakova   +5 more
doaj   +1 more source

Hybridoma technology; advancements, clinical significance, and future aspects

open access: yesJournal of Genetic Engineering and Biotechnology, 2021
Background Hybridoma technology is one of the most common methods used to produce monoclonal antibodies. In this process, antibody-producing B lymphocytes are isolated from mice after immunizing the mice with specific antigen and are fused with immortal ...
Sanchita Mitra, Pushpa Chaudhary Tomar
doaj   +1 more source

Efficient Cross-Screening and Characterization of Monoclonal Antibodies against Marek’s Disease Specific Meq Oncoprotein Using CRISPR/Cas9-Gene-Edited Viruses

open access: yesViruses, 2023
Marek’s disease (MD) caused by pathogenic Marek’s disease virus type 1 (MDV−1) is one of the most important neoplastic diseases of poultry. MDV−1-encoded unique Meq protein is the major oncoprotein and the availability of Meq-specific monoclonal ...
Man Teng   +7 more
doaj   +1 more source

Fluorescent Microtiter Screening Assay for Immunocytochemically Reactive Antibodies

open access: yesBioTechniques, 1997
We have developed a rapid in situ screening procedure that enables prescreening of hundreds of hybridomas in a 96-well format. The procedure involves fluorescence immunostaining of cells cultured in 96-well plates and the use of a fluorescence plate ...
J.-L. Su   +5 more
doaj   +1 more source

Expression of a recombinant ASFV P30 protein and production of monoclonal antibodies

open access: yesOpen Veterinary Journal, 2023
Background: African Swine Fever (ASF) is an infectious disease that affects domestic pig and wild boar populations. The African Swine Fever Virus (ASFV) has a genome characterized by a very complex DNA (170-193 kb) that encodes for more than 200 ...
Rosrio Liberti   +10 more
doaj   +1 more source

Manufacturing of Hybridomas-Producers of Monoclonal Anti-Bodies to Tularemia Agent Antigens

open access: yesПроблемы особо опасных инфекций, 2015
Carried out have been two experimental studies on hybridization of mouse myeloma cells and lymphocytes of BALB/c mice immunized with inactivated microbe Francisella tularensis cultures.
O. O. Fomenkov   +9 more
doaj   +1 more source

Two better cell lines for making hybridomas expressing specific T cell receptors.

open access: yesJournal of Immunology, 1989
Two variants of the AKR thymoma BW5147 have been isolated which can no longer express functional TCR alpha- and beta-chains. By generating hybridomas with these variant fusion lines, TCR of any normal T lymphocyte, including TCR-gamma/delta, can be ...
Janicé White   +6 more
semanticscholar   +1 more source

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