Results 31 to 40 of about 59,735 (325)
Immunogenomic engineering of a plug-and-(dis)play hybridoma platform
Nature Communications, 2016 Hybridomas are widely used for antibody screening and production due to their genetic stability and rapid proliferation. Here the authors demonstrate the rapid reprogramming of antibody specificity in hybridomas using CRISPR-Cas9.
Mark Pogson +4 more
doaj +1 more source
Viruses, 2023 Marek’s disease (MD) caused by pathogenic Marek’s disease virus type 1 (MDV−1) is one of the most important neoplastic diseases of poultry. MDV−1-encoded unique Meq protein is the major oncoprotein and the availability of Meq-specific monoclonal ...
Man Teng +7 more
doaj +1 more source
Hybridoma technology; advancements, clinical significance, and future aspects
Journal of Genetic Engineering and Biotechnology, 2021 Background Hybridoma technology is one of the most common methods used to produce monoclonal antibodies. In this process, antibody-producing B lymphocytes are isolated from mice after immunizing the mice with specific antigen and are fused with immortal ...
Sanchita Mitra, Pushpa Chaudhary Tomar
doaj +1 more source
A New Workflow to Generate Monoclonal Antibodies against Microorganisms
Applied Sciences, 2021 Monoclonal antibodies are used worldwide as highly potent and efficient detection reagents for research and diagnostic applications. Nevertheless, the specific targeting of complex antigens such as whole microorganisms remains a challenge.
Markus Göthel +5 more
doaj +1 more source
Function of B cells expressing a human immunoglobulin M rheumatoid factor autoantibody in transgenic mice. [PDF]
, 1993 We have generated transgenic mice that express the immunoglobulin (Ig)M heavy chain and kappa light chain genes coding for a human IgM rheumatoid factor (RF), Les.
Carson, DA +6 more
core +2 more sources
Journal of Immunology Research, 2016 The current study was to prepare a mouse-derived antibody against the angiotensin II type 1 receptor (AT1-mAb) based on monoclonal antibody technology, to provide a foundation for research on AT1-AA-positive diseases.
Mingming Wei +5 more
doaj +1 more source
Проблемы особо опасных инфекций, 2014 Objective of the research was to use immunoblotting for studies of epitope targeting in monoclonal antibodies to 200 kDa Burkholderia pseudomallei antigen, which are synthesized by hybridomas-producers from the two collections in the laboratory of ...
I. I. Korsakova +5 more
doaj +1 more source
Expression of a recombinant ASFV P30 protein and production of monoclonal antibodies
Open Veterinary Journal, 2023 Background: African Swine Fever (ASF) is an infectious disease that affects domestic pig and wild boar populations. The African Swine Fever Virus (ASFV) has a genome characterized by a very complex DNA (170-193 kb) that encodes for more than 200 ...
Rosrio Liberti +10 more
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Fluorescent Microtiter Screening Assay for Immunocytochemically Reactive Antibodies
BioTechniques, 1997 We have developed a rapid in situ screening procedure that enables prescreening of hundreds of hybridomas in a 96-well format. The procedure involves fluorescence immunostaining of cells cultured in 96-well plates and the use of a fluorescence plate ...
J.-L. Su +5 more
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A hybridoma-derived monoclonal antibody with high homology to the aberrant myeloma light chain.
PLoS ONE, 2021 The identification of antibody variable regions in the heavy (VH) and light (VL) chains from hybridomas is necessary for the production of recombinant, sequence-defined monoclonal antibodies (mAbs) and antibody derivatives.
Ghasidit Pornnoppadol +6 more
doaj +1 more source