A brief history and future directions of dielectrophoretic filtration: A review
ELECTROPHORESIS, EarlyView.Abstract Dielectrophoresis (DEP) is an electrokinetic effect first studied in the early 20th century. Since then, DEP has gained significant interest in research, owing to its ability to solve particle separation problems in various industries. Dielectrophoretic filtration (DEP filtration) is a separation method using DEP to filter a wide range of ...
Mary Clare O'Donnell +2 more
wiley +1 more source
Monoclonal Antibodies of a Diverse Isotype Induced by an O-Antigen Glycoconjugate Vaccine Mediate In Vitro and In Vivo Killing of African Invasive Nontyphoidal Salmonella. [PDF]
, 2015 Nontyphoidal Salmonella (NTS), particularly Salmonella enterica serovars Typhimurium and Enteritidis, is responsible for a major global burden of invasive disease with high associated case-fatality rates.
core +1 more source
Trinitrophenol Reactive T-Cell Hybridomas Recognize Antigens That Require Antigen Processing [PDF]
, 1994 Protein antigens must be taken up, processed, and displayed on the surface of antigen-presenting cells in association with major histocompatibility complex molecules before they can be recognized by T cells.
Bigby, Michael +5 more
core +1 more source
Imaging Extracellular Protein Concentration with Nanoplasmonic Sensors [PDF]
arXiv, 2015 Extracellular protein concentrations and gradients queue a wide range of cellular responses, such as cell motility and division. Spatio-temporal quantification of these concentrations as produced by cells has proven challenging. As a result, artificial gradients must be introduced to the cell culture to correlate signal and response.
arxiv
Multi‐cycle high‐throughput growth media optimization using batch Bayesian optimization
Journal of Chemical Technology &Biotechnology, EarlyView.Abstract BACKGROUND The optimization of growth media is a crucial step in enhancing microbial growth and product formation in industrial bioprocesses. While Design of Experiments (DoE) is widely used for this purpose, it has inherent limitations, particularly in capturing complex interactions between media components and managing the high number of ...
Frédéric M. Lapierre +5 more
wiley +1 more source
PLoS ONE, 2015 B cell hybridomas are an important source of monoclonal antibodies. In this paper, we developed a high-throughput method to characterize mouse IgG antibodies using surface plasmon resonance technology.
Haolin Liu +9 more
doaj +1 more source
Validation of an enzyme-linked immunosorbent assay for the quantification of human IgG directed against the repeat region of the circumsporozoite protein of the parasite Plasmodium falciparum. [PDF]
, 2012 BACKGROUND: Several pre-erythrocytic malaria vaccines based on the circumsporozoite protein (CSP) antigen of Plasmodium falciparum are in clinical development.
Cambron, Pierre +10 more
core +2 more sources
Cell growth cycle block of T cell hybridomas upon activation with antigen
Journal of Experimental Medicine, 1987 Stimulation of antigen-specific T cell hybridomas with the appropriate antigen/MHC combination, at concentrations that resulted in the secretion of the lymphokine interleukin 2, resulted in a dose-dependent decrease in both [3H]thymidine incorporation ...
J. Ashwell +3 more
semanticscholar +1 more source
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, EarlyView.Different insect species exhibit remarkable diversity in the developmental trajectories from fertilized eggs to hatching larvae. For three myrmicine ant species, we observe a novel combination of a developmental features: small embryonic primordia, a mode of embryogenesis termed short/intermediate germ‐band development, and progressive establishment of
Chi‐Chun Fang +5 more
wiley +1 more source
Visualization and phenotyping of proinflammatory antigen-specific T cells during collagen-induced arthritis in a mouse with a fixed collagen type II-specific transgenic T-cell receptor beta-chain [PDF]
, 2010 Introduction: The Vbeta12-transgenic mouse was previously generated to investigate the role of antigen-specific T cells in collagen-induced arthritis (CIA), an animal model for rheumatoid arthritis. This mouse expresses a transgenic collagen type II (CII)
Baecklund, Johan +7 more
core +2 more sources