Results 81 to 90 of about 59,735 (325)

Development of Monoclonal Antibody-Based EIA for Tetranor-PGDM which Reflects PGD2 Production in the Body

Journal of Immunology Research, 2021
Tetranor-PGDM is a metabolite of PGD2. Urinary tetranor-PGDM levels were reported to be increased in some diseases, including food allergy, Duchenne muscular dystrophy, and aspirin-intolerant asthma. In this study, we developed a monoclonal antibody (MAb)
Nanae Nagata, Sakura Masuko, Rikako Inoue, Tatsuro Nakamura, Kosuke Aritake, Takahisa Murata   +5 more
doaj   +1 more source

New simplified molecular design for functional T cell receptor [PDF]

, 1993
We have produced a chimeric single-chain T cell receptor (TcR) that combines the specific antibody recognition function and TcR/CD3 signaling properties within the same polypeptide chain.
Clevers, Cosson, Eshhar, Frank, Gillis, Gunning, Hackett, Huston, Ihle, Irving, Lanzavecchia, Leo, Mosmann, Oi, Romeo, Sterz, Tada, Traunecker, Walker, Wegener, Weissman, Weissman, White, Zamoyska   +23 more
core   +1 more source

Recombinant Monoclonal Antibodies for Detecting the Tubulin Post‐Translational Modifications Glutamylation and Lysine‐40 Acetylation

Cytoskeleton, EarlyView.
ABSTRACT Post‐translational modifications (PTMs) to tubulin subunits in microtubule filaments are thought to comprise a component of the tubulin code that specifies microtubule functions in cell physiology and animal development. Acetylation of Lysine‐40 (K40) on α‐tubulin (αTub‐K40ac) and glutamylation of both α‐ and β‐tubulin are two tubulin PTMs of ...
Lynne Blasius, Ezekiel C. Thomas, Erik H. Collet, Erin Jenson, Chad G. Pearson, Ryoma Ohi, Kristen J. Verhey   +6 more
wiley   +1 more source

A Rapid Method to Characterize Mouse IgG Antibodies and Isolate Native Antigen Binding IgG B Cell Hybridomas.

PLoS ONE, 2015
B cell hybridomas are an important source of monoclonal antibodies. In this paper, we developed a high-throughput method to characterize mouse IgG antibodies using surface plasmon resonance technology.
Haolin Liu, Janice White, Frances Crawford, Niyun Jin, Xiangwu Ju, Kangtai Liu, Chengyu Jiang, Philippa Marrack, Gongyi Zhang, John W Kappler   +9 more
doaj   +1 more source

Interaction of Streptavidin-Based Peptide-MHC Oligomers (Tetramers) with Cell-Surface T Cell Receptors [PDF]

, 2011
he binding of oligomeric peptide–MHC (pMHC) complexes to cell surface TCR can be considered to approximate TCR–pMHC interactions at cell-cell interfaces.
Adam S. Chervin, Arup K. Chakraborty, David M. Kranz, Fahmy, Herman N. Eisen, Hornick, Jennifer D. Stone, Maxim N. Artyomov, Udaka   +8 more
core   +1 more source

Cell growth cycle block of T cell hybridomas upon activation with antigen

Journal of Experimental Medicine, 1987
Stimulation of antigen-specific T cell hybridomas with the appropriate antigen/MHC combination, at concentrations that resulted in the secretion of the lymphokine interleukin 2, resulted in a dose-dependent decrease in both [3H]thymidine incorporation ...
J. Ashwell, R. E. Cunningham, P. Noguchi, D. Hernandez   +3 more
semanticscholar   +1 more source

Squamate ventricular cardiomyocytes: Ploidy, proliferation, and heart muscle cell size in the leopard gecko (Eublepharis macularius)

Developmental Dynamics, EarlyView.
Abstract Background While heart function is broadly conserved across vertebrates, the cellular phenotype of muscle cells (cardiomyocytes) varies across taxa and throughout ontogeny. Emerging evidence suggests that some attributes may correlate with the capacity for spontaneous cardiomyocyte replacement following injury.
Kathy Jacyniak, Karemna Barrera Jaimes, Minh Hanh Doan, Jordyn M. Chartrand, Matthew K. Vickaryous   +4 more
wiley   +1 more source

α-Lactosylceramide Protects Against iNKT-Mediated Murine Airway Hyperreactivity and Liver Injury Through Competitive Inhibition of Cd1d Binding. [PDF]

, 2019
Invariant natural killer T (iNKT) cells, which are activated by T cell receptor (TCR)-dependent recognition of lipid-based antigens presented by the CD1d molecule, have been shown to participate in the pathogenesis of many diseases, including asthma and ...
Chang, Ya-Jen, Chi, Po-Yu, Gervay-Hague, Jacquelyn, Han, Yun-Chiann, Hsieh, Hsiao-Wu, Kao, Hsien-Neng, Lai, Alan Chuan-Ying, Thio, Christina Li-Ping   +7 more
core  

Sponge Hybridomas: Applications and Implications [PDF]

Integrative and Comparative Biology, 2013
Many sponge-derived natural products with applications to human health have been discovered over the past three decades. In vitro production has been proposed as one biological alternative to ensure adequate supply of marine natural products for preclinical and clinical development of drugs. Although primary cell cultures have been established for many
Pomponi, S.A., Jevitt, A., Patel, J., Diaz, M.C.   +3 more
openaire   +3 more sources

Activation-induced cell death in T cell hybridomas is due to apoptosis. Morphologic aspects and DNA fragmentation.

Journal of Immunology, 1990
Some T cell hybridomas, upon activation via the TCR, rapidly undergo cell death. In this paper, we demonstrate that this activation-induced cell death (AICD) is accompanied by morphologic changes seen at the electron and light microscopy levels. The most
Yufang Shi, M. Szalay, L. Paskar, B. Sahai, M. I. Boyer, Bhagirath Singh, D. Green   +6 more
semanticscholar   +1 more source