Results 91 to 100 of about 19,999 (251)

Optimization of electrofusion parameters for producing hybridomas synthesizing human monoclonal antibodies

, 2021
Monoclonal antibodies are an established treatment for many diseases, including cancer, toxic conditions and infectious diseases. The goal of this work was to optimize the conditions for obtaining hybridomas secreting human monoclonal IgG antibodies.
Silkina M. V.
core  

Polymeric α‐Hairpinin Allergens Induce a Functional Response via a Single Antibody

Allergy, EarlyView.
Gage O. Leighton, Taylor M. Cosey, Emmanuel R. De Diavoukana, Isabelle R. Lytle, Alexander C. Y. Foo, Lars C. Pedersen, Scott A. Gabel, Robert M. Petrovich, Min Shi, Venu Aruva, Paige Creeks, Joyce J. W. Wong, Jian Zhang, R. Stokes Peebles Jr., Derek Croote, Scott A. Smith, Geoffrey A. Mueller   +16 more
wiley   +1 more source

Utility of local capillary supply indices: Insights from computational image‐based modelling

The Journal of Physiology, EarlyView.
Abstract figure legend Local capillary distribution and fibre geometry influence oxygen availability in skeletal muscle. Image‐based modelling of tissue PO2${{P}_{{{{\mathrm{O}}}_2}}}$ shows that area‐based measures of capillary supply – the local capillary density (LCDi) and local maximum diffusion distance (Dmax,i) – most accurately represent the ...
Abdullah A. Al‐Shammari, Roger W. P. Kissane, Ali Ashkanani, Ashraf Al Madhoun, Fahd Al‐Mulla, Eamonn A. Gaffney, Stuart Egginton   +6 more
wiley   +1 more source

Induction of long-term but reversible unresponsiveness after activation of murine T cell hybridomas.

, 1991
T cell receptor (TCR)-mediated stimulation of murine T cell hybridomas induces cell activation and lymphokine production. We have observed that following productive activation, T cell hybridomas become refractory to a subsequent stimulation ...
Kaufman, Marcelle, Leo, Oberdan, Andris, Fabienne, Van Mechelen, Marcelle, Legrand, N, Dubois, Patrice, Urbain, Jacques   +6 more
core   +1 more source

Antioxidant supplementation blunts the proteome response to 3 weeks of sprint interval training preferentially in human type 2 muscle fibres

The Journal of Physiology, EarlyView.
Abstract figure legend Sprint interval training (SIT) is a popular time‐efficient type of endurance training. Healthy young men performed nine SIT sessions (4–6 × 30 s all‐out cycling sprints) over 3 weeks while being supplemented with antioxidants (high doses of vitamins C and E) or placebo. Muscle biopsies taken before and after the first SIT session
Victoria L. Wyckelsma, Marta Murgia, Sigitas Kamandulis, Stefano Gastaldello, Marius Brazaitis, Audrius Snieckus, Nerijus Eimantas, Mati Pääsuke, Sebastian Edman, William Apro, Daniel C. Andersson, Håkan Westerblad, Tomas Venckunas   +12 more
wiley   +1 more source

Introduction of Genes for Drug Tolerance into Human-Human Hybridomas by Electroporation

, 1990
We examined the effect of gene transfer into human-human hybridomas by electroporation on their production of immunoglobulin. Drug-resistant hyblidomas were easily obtained after transfection of hybridomas with the pSV2-neo or Eco gpt-E1A plasmid DNA by ...
立花, 宏文, Shirahata, Sanetaka, 白畑, 実隆, Tachibana, Hirofumi, Yamada, Koji, 村上, 浩紀, Murakami, Hiroki, 山田, 耕路   +7 more
core  

Cell-autonomous Fas (CD95)/Fas-ligand interaction mediates activation-induced apoptosis in T-cell hybridomas

, 1995
A number of murine T-cell hybridomas undergo apoptosis within a few hours of activation by specific antigens, mitogens, antibodies against the T-cell antigen receptor, or a combination of phorbol ester and calcium ionophore.
Nam Jin Yoo, LaFace, Drake, Force, Walker, Lynch, David H., Brunner, Thomas, Artin Mahboubi, Douglas R. Green, Ware, Carl F., Carl F. Ware, Force, Walker R., Thomas Brunner, Green, Douglas R., Martin, Seamus J., Yoo, Nam Jin, Walker R. Force, Green, Douglas, Echeverri, Fernando, David H. Lynch, Mogil, Rona, Mogil, Rona J., Seamus J. Martin, Martin, Seamus, Lynch, David, Drake LaFace, Mahboubi, Artin, Rona J. Mogil, Fernando Echeverri, Ware, Carl   +27 more
core   +1 more source

Resistance training load does not determine resistance training‐induced hypertrophy across upper and lower limbs in healthy young males

The Journal of Physiology, EarlyView.
Abstract figure legend In healthy young males, we found that skeletal muscle hypertrophy following a period of resistance exercise training (RET) was consistent within and between subjects, as assessed using a variety of established methods. The skeletal muscle hypertrophic response was independent of external load.
Matthew J. Lees, Jonathan C. Mcleod, Robert W. Morton, Brad S. Currier, Matthew D. Fliss, Sean R. McKellar, Rajbir S. Sidhu, Ben N. Stansfield, Erin K. Webb, Chris McGlory, Jatin G. Burniston, Stuart M. Phillips   +11 more
wiley   +1 more source

Diving into the unknown: Evidence of enhanced skeletal muscle lactate efflux potential and blood–muscle exchange in competitive breath‐hold divers

The Journal of Physiology, EarlyView.
Abstract figure legend Competitive breath‐hold divers exhibit skeletal muscle characteristics indicative of enhanced blood‐muscle exchange capacity and increased lactate efflux potential. Top left: Participant characteristics, matched for age, height, body mass, and maximal oxygen uptake (V̇O2max${\dot{\mathrm{V}}}\rm{O}_{\rm{2max}}$).
Antonis Elia, Matthew Lees, John O'Hara, Nathan Hodson, Jordan Acheson, Oliver Wilson, Paul Parker, Matthew Barlow   +7 more
wiley   +1 more source

Production of Monoclonal Antibodies Against the Challenge Strain of Infectious Laryngotracheitis Virus of Chickens and Their Use in an Indirect Immunofluorescent Diagnostic Test [PDF]

Pakistan Veterinary Journal, 2010
The objective of the present research was to produce monoclonal antibodies (MCAs) against the USDA challenge strain of infectious laryngotracheitis virus and to perform an initial investigation of their use in an indirect immunofluorescence diagnostic ...
Ferhat Abbas*, James Andreasen1, Rockey Becker1, Masroor Ahmed, M Arif Awan, Abdul Wadood and Anita Sonn1
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