A fast, miniaturised in-vitro assay developed for quantification of lipase enzyme activity
Journal of Enzyme Inhibition and Medicinal Chemistry, 2019 The discovery of allosteric modulators is a multi-disciplinary approach, which is time- and cost-intensive. High-throughput screening combined with novel computational tools can reduce these factors. Thus, we developed an enzyme activity assay, which can
Ariane Menden +7 more
doaj +1 more source
Frontiers in Plant Science, 2016 Various perennial C4 grass species have tremendous potential for use as lignocellulosic biofuel feedstocks. Currently available grasses require costly pre-treatment and exogenous hydrolytic enzyme application to break down complex cell wall polymers ...
Jonathan eWillis +2 more
doaj +1 more source
Chemistry, 2023 The de novo design and synthesis of peptide-based biocatalysts that can mimic the activity of natural enzymes is an exciting field with unique opportunities and challenges.
Soumya Patra +4 more
doaj +1 more source
A yeast gene (BLH1) encodes a polypeptide with high homology to vertebrate bleomycin hydrolase, a family member of thiol proteinases [PDF]
, 1993 We have purified bleomycin hydrolase from yeast (molecular mass 55 000 Da). Using protein sequence-derived degenerate oligonucleotide primers and amplification by polymerase chain reaction, the yeast gene BLH1 was isolated and characterized.
Akiyama +18 more
core +1 more source
Enzymatic Modification of Pomace Olive Oil with Natural Antioxidants: Effect on Oxidative Stability
Biomolecules, 2023 Enzymatic lipophilization has been proposed as a cost-effective strategy to produce new liposoluble antioxidant compounds. In this study, modified oils rich in structured phenolipids were prepared via one-pot enzymatic acylation of hydroxytyrosol (HTYR),
Renia Fotiadou +5 more
doaj +1 more source
Strigolactone perception and deactivation by a hydrolase receptor DWARF14
Nature Communications, 2019 The perception mechanism for the strigolactone (SL) class of plant hormones has been a subject of debate because their receptor, DWARF14 (D14), is an α/β-hydrolase that can cleave SLs. Here we show via time-course analyses of SL binding and hydrolysis by
Yoshiya Seto +21 more
semanticscholar +1 more source
Crystallization and preliminary X-ray analysis of neoagarobiose hydrolase from Saccharophagus degradans 2-40 [PDF]
, 2009 Many agarolytic bacteria degrade agar polysaccharide into the disaccharide unit neoagarobiose [O-3,6-anhydro-α-L-galactopyranosyl-(1→3)-D-galactose] using various β-agarases.
Araki +18 more
core +2 more sources
Mammalian soluble epoxide hydrolase is identical to liver hepoxilin hydrolase [PDF]
Journal of Lipid Research, 2011 Hepoxilins are lipid signaling molecules derived from arachidonic acid through the 12-lipoxygenase pathway. These trans-epoxy hydroxy eicosanoids play a role in a variety of physiological processes, including inflammation, neurotransmission, and formation of skin barrier function.
Cronin, A, Decker, M, Arand, Michael
openaire +5 more sources
Structural insight into catalytic mechanism of PET hydrolase
Nature Communications, 2017 PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics.
Xu Han +9 more
semanticscholar +1 more source
Soluble Epoxide Hydrolase Inhibition for Ocular Diseases: Vision for the Future [PDF]
, 2019 Ocular diseases cause visual impairment and blindness, imposing a devastating impact on quality of life and a substantial societal economic burden. Many such diseases lack universally effective pharmacotherapies.
Corson, Timothy W., Park, Bomina
core +1 more source