Results 61 to 70 of about 183,842 (201)

Same hydrolase knockouts, different outcomes: CRISPR/Cas9 editing workflow shapes resulting cellular phenotype and transcriptome in CHO

Frontiers in Bioengineering and Biotechnology
CRISPR/Cas9-based multi-gene knockout strategies are widely applied to engineer Chinese hamster ovary (CHO) cells, yet the impact of editing workflow on cellular outcomes remains poorly understood. Here, we systematically compare sequential and multiplex
Linus Weiß, Stefan Schneider, Kerstin Otte, Simon Fischer   +3 more
doaj   +1 more source

2-hydroxymuconic semialdehyde hydrolase original MUSCLE alignment.txt

, 2013
2-hydroxymuconic semialdehyde hydrolase original MUSCLE alignment ...
Leadbetter, Jared R., Lucey, Kaitlyn S., Kaitlyn S. Lucey (3315138), Jared R. Leadbetter (223388)   +3 more
core   +1 more source

TECHNOLOGY OF MISCANTHUS BIOMASS SACCHARIFICATION WITH COMMERCIALLY AVAILABLE ENZYMES

Вавиловский журнал генетики и селекции, 2015
We analyzed the possibility of using commercially available enzymes with cellulosolytic activity for saccharification of miscanthus biomass, Soranovsky variety, a new crop registered in Russia in 2013, in comparison to the saccharification of biomasses ...
T. N. Goryachkovskaya, K. V. Starostin, I. A. Meshcheryakova, N. M. Slynko, S. E. Peltek   +4 more
doaj  

Probing the Active Site of Class 3 L-Asparaginase by Mutagenesis: Mutations of the Ser-Lys Tandems of ReAV

Biomolecules
The ReAV enzyme from Rhizobium etli, a representative of Class 3 L-asparaginases, is sequentially and structurally different from other known L-asparaginases. This distinctiveness makes ReAV a candidate for novel antileukemic therapies.
Kinga Pokrywka, Marta Grzechowiak, Joanna Sliwiak, Paulina Worsztynowicz, Joanna I. Loch, Milosz Ruszkowski, Miroslaw Gilski, Mariusz Jaskolski   +7 more
doaj   +1 more source

2-hydroxymuconic semialdehyde hydrolase ARB output.nex

, 2013
2-hydroxymuconic semialdehyde hydrolase ARB output ...
Leadbetter, Jared R., Lucey, Kaitlyn S., Kaitlyn S. Lucey (3315138), Jared R. Leadbetter (223388)   +3 more
core   +1 more source

Illuminating a biologics development challenge: systematic characterization of CHO cell-derived hydrolases identified in monoclonal antibody formulations

mAbs
Monoclonal antibodies (mAb) and other biological drugs are affected by enzymatic polysorbate (PS) degradation that reduces product stability and jeopardizes the supply of innovative medicines.
Melanie Maier, Linus Weiß, Nikolas Zeh, Valerie Schmieder-Todtenhaupt, Alireza Dehghani, Marius Nicolaus Felix, Daniel Heinzelmann, Benjamin Lindner, Moritz Schmidt, Joey Studts, Patrick Schulz, Bernd Reisinger, Kerstin Otte, Matthias Franzreb, Daniel Lakatos, Simon Fischer   +15 more
doaj   +1 more source

Sequences of designed variants of Zn-Hydrolase.

, 2014
Sequences of designed variants of Zn-Hydrolase.
Kinshuk Raj Srivastava (562363), Susheel Durani (504167)   +1 more
core   +1 more source

Ce‐Doped Lignin‐Based Nanozyme with Heat‐Activated Hydrolase Activity

Advanced Science
Nanozymes are powerful alternatives to natural enzymes, yet the sustainable design of hydrolase nanozymes using renewable ligands remains underexplored.
Xin Liu, Lijun Li, Xue Zhang, Alireza Ashori, Fubao Sun, Feng Xu, Xueming Zhang, Yao Chen   +7 more
doaj   +1 more source

Phenolic supplements: testing an approach to limit Sphagnum decomposition in a Sphagnum farming system

Frontiers in Earth Science
Phenolic compounds have been proposed to influence decomposition by inhibiting extracellular enzyme activities, as described in the enzymic latch mechanism (ELM).
Talal Asif, Line Rochefort, Chris Freeman, Christian Dunn, Hojeong Kang, Mélina Guêné-Nanchen   +5 more
doaj   +1 more source

DWARF14, A Receptor Covalently Linked with the Active Form of Strigolactones, Undergoes Strigolactone-Dependent Degradation in Rice

Frontiers in Plant Science, 2017
Strigolactones (SLs) are the latest confirmed phytohormones that regulate shoot branching by inhibiting bud outgrowth in higher plants. Perception of SLs depends on a novel mechanism employing an enzyme-receptor DWARF14 (D14) that hydrolyzes SLs and ...
Qingliang Hu, Qingliang Hu, Yajun He, Yajun He, Lei Wang, Simiao Liu, Xiangbing Meng, Guifu Liu, Yanhui Jing, Mingjiang Chen, Xiaoguang Song, Liang Jiang, Hong Yu, Bing Wang, Jiayang Li, Jiayang Li   +15 more
doaj   +1 more source