Continuous Spectrophotometric Assay for Defluorinase and Dechlorinase Activities With α-Halocarboxylic Acids. [PDF]
Microb BiotechnolMicrobial hydrolytic defluorinases and dechlorinases assayed by coupling the reaction product to NAD‐linked dehydrogenases will facilitate screening for and characterising new and known enzymes. ABSTRACT Many environmental pollutants have a fluorine or chlorine atom on a carbon atom adjacent to a carboxylic acid.
Ronnander M +6 more
europepmc +2 more sources
Combination of a UPO-Based Epoxidation With a Subsequent Ring-Opening Reaction for the Synthesis of Amino Alcohols. [PDF]
ChembiochemThe synthesis of aromatic amino alcohols via a combination of an unspecific peroxygenase‐catalysed reaction, oxyfunctionalization, and addition of selected nucleophiles and electrophiles in a highly atom‐efficient manner was investigated. This study presents the design to aim for an atom‐efficient chemo‐enzymatic synthesis route towards aromatic amino ...
Last S +3 more
europepmc +2 more sources
EnzRetro: Enzymatic Retrosynthetic Planning With Site-Specific Reaction Edits Based on Sequence Generative Architecture. [PDF]
Exploration (Beijing)EnzRetro bridges retrosynthesis planning and enzymatic engineering through site‐specific reaction edits. This end‐to‐end framework integrates pathway synthesis and enzyme identification into a unified process, achieving superior accuracy and enabling the efficient reconstruction of diverse biosynthetic pathways.
Cao Y +5 more
europepmc +2 more sources
Engineering Enzymes for Environmental Sustainability
Angewandte Chemie, Volume 135, Issue 52, December 21, 2023., 2023 This review highlights how engineered enzymes have been developed and implemented to help address environmental challenges. Topics include the use of engineered enzymes for improving carbon capture and utilisation, bioremediation, plastic deconstruction, and renewable feedstock generation.
Emily Radley +5 more
wiley +2 more sources
Identification of genes coding for hydrolytic dehalogenation in the metagenome derived from a denitrifying 4-chlorobenzoate degrading consortium. [PDF]
FEMS microbiology letters, 2008 A metagenomic approach was taken to investigate the genetic basis for the ability of an anaerobic consortium to grow on either 4-chlorobenzoate or 4-bromobenzoate under denitrifying conditions. Degenerate PCR primers were designed for the family of 4-chlorobenzoyl-CoA dehalogenase genes.
Jong-Chan, Chae +2 more
openaire +2 more sources
Designing Enzymatic Reactivity with an Expanded Palette. [PDF]
ChembiochemInnovation in biocatalysis is rapidly increasingly the diversity of catalytic reactivity that can be mediated by enzymes, addressing a key bottleneck for their widespread adoption in industrial chemical synthesis. A key approach to this is building enzymes with unnatural catalytic components that provide an expanded palette with new possibilities for ...
Leveson-Gower RB.
europepmc +2 more sources
Hydrolytic dehalogenation of the chlorothalonil isomer o-tetrachlorophthalonitrile by Pseudochrobactrum sp. BSQ-1 [PDF]
International Biodeterioration & Biodegradation, 2016 Abstract Microbial dehalogenation plays key roles in the biodegradation and detoxification of halogenated aromatics. Although the hydrolytic dehalogenation of halogenated aliphatic hydrocarbons and carboxylic acids has been extensively studied, there are few reports on the hydrolytic dehalogenation of halogenated aromatics. In this study, the aerobic
Xiaomei Liu +7 more
openaire +1 more source
Biotransformation of halogenated compounds by lyophilized cells of Rhodococcus erythropolis in a continuous solid-gas biofilter [PDF]
, 2004 The irreversible hydrolysis of 1-chlorobutane to 1-butanol and HCl by lyophilized cells of Rhodococcus erythropolis NCIMB 13064, using a solid–gas biofilter, is described as a model reaction.
Erable, Benjamin +4 more
core +5 more sources
Bioremediation of halogenated compounds: comparison of dehalogenating bacteria and improvement of catalyst stability [PDF]
, 2006 Five bacterial strains were compared for halogenated compounds conversion in aqueous media. Depending on the strain, the optimal temperature for dehalogenase activity of resting cells varied from 30 to 45 degrees C, while optimal pH raised from 8.4 to 9 ...
Erable, Benjamin +4 more
core +4 more sources
Evolutionary obstacles and not C-F bond strength make PFAS persistent. [PDF]
Microb BiotechnolC–F bonds can be cleaved by microbes. However, evolution must overcome many obstacles to biodegrade PFAS. Abstract The fate of organic matter in the environment, including anthropogenic chemicals, is largely predicated on the enzymatic capabilities of microorganisms.
Wackett LP.
europepmc +2 more sources